Antioxidant and in vitro antidiabetic activities of Peperomia pellucida (L.) Kunth extract

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Tran Thanh Men
La Thi Kim Tu
Nguyen Thi Kim Anh
Huynh Hong Phien
Nguyen Thi Bich Nhu
Nguyen Thi To Uyen
Ngo Thi Anh Thu
Tran Ngoc Quy
Do Tan Khang


Peperomia pellucida (L.) is commonly used as a herbal plant. Its effectiveness in treating inflammatory diseases, digestive disorders, and cancer in tropical and subtropical countries was introduced, especially in field of folk medicine. However, this plant species has not been studied widely in Vietnam, especially for its biological activities. This study was done to determine the antioxidant capacity of P. pellucida by using in vitro and in vivo methods, as well as its inhibitory ability to α-amylase enzyme activity. The total polyphenolic and flavonoid contents of P. pellucida extract were reported to be 359.91±0.77 mg GAE/g and 200.28±1.23 mg QE/g extract, respectively. The results showed the in vitro antioxidant activity of P. pellucida extract in four methods, including DPPH, and ABTS.+, RP and TAC, had EC50 values of 730.34 μg/mL, 84.33 μg/mL, 95.28 μg/mL, respectively, and Abs0.5 value of 114.73 μg/mL. Under H2O2-induced oxidative stress, fruit flies that were raised in the feed medium supplemented with a concentration of 1 mg/mL of P. pellucida extract showed their average survival time, 50% survival time, and 10% survival time at 1.6 times, 1.8 times, and 1.62 times, respectively, higher than those of the control treatment. The ability to inhibit the α-amylase activity in P. pellucida extract was determined with an EC50 value of 115.32±2.65 μg/mL compared with the commercial drug of 18.67±0, 01 μg/mL. The research results showed that P. pellucida is a potential species in the study of natural compounds with antioxidant and antidiabetic activities.


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Thanh Men, T. ., Thi Kim Tu, L. ., Thi Kim Anh, N. ., Hong Phien, H. ., Thi Bich Nhu, N. ., Thi To Uyen, N. ., Thi Anh Thu, N. ., Ngoc Quy, T. ., & Tan Khang, D. . (2022). Antioxidant and in vitro antidiabetic activities of Peperomia pellucida (L.) Kunth extract: Veterinary Integrative Sciences, 20(3), 683–693. Retrieved from
Research Articles


Aba, P.E., Asuzu, I.U., 2018. Mechanisms of actions of some bioactive antidiabetic principles from phytochemicals of medicinal plants: A review. Indian J. Nat. Prod. Resour.9(2), 85-96.

Adhitia, A.M., Octaviani, A.N., Basah, K., Mun’im, A., 2017. Effect of gamma irradiation on angiotensin converting enzyme inhibition, antioxidant activity, total phenoli ccompound and total flavonoid of Peperomia pellucida herbs extract. Phcog. J. 9(2),244-248.

Ahmad, I., Yanuar, A., Mulia, K., Mun'im, A., 2018. Extraction of polyphenolic content from Peperomia pellucida (L) Kunth herb with 1-ethyl-3-methylimidazolium bromide. Indian J. Pharm. Sci. 79(6), 1013-1017.

Arrigoni-Blank Mde, F., Oliveira, R.L., Mendes, S.S., Silva Pde, A., Antoniolli, A.R., Vilar,J.C., Cavalcanti, S.C., Blank, A.F., 2002. Seed germination, phenology, and antiedematogenic activity of Peperomia pellucida (L.) H.B.K. BMC Pharmacol. 2(12), 1-8.

Buhian, S.P.C., Oyong, G.G., Cabrera, E.C., 2019. Cytotoxicity of Peperomia pellucida (L.) HBK extracts on cancer cell lines and their effects on cfos and cjun genes. Philipp.Agric. Sci. 102(1), 24-32.

Dewanto, V., Wu, X., Adom, K.K., Liu, R.H., 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem. 50(10), 3010-3014.

DiSilvestro, R.A., 2000. Flavonoids as antioxidants. In: Wildman, R.E. (Eds.), Handbook of nutraceuticals and functional foods. Boca Raton, FL, CRC Press, pp. 127-142.

Fatiha, M., Fatma, B., Awatif, B., Nesrine, A., Noureddine, D., 2018. Antidiabetic bioactive compounds from plants. Med. Technol. J. 2(2), 199-214.

Florence, N.T., Huguette, S.T.S., Hubert, D.J., Raceline, G.K., Desire, D.D.P., Pierre, K.,Theophile, D., 2017. Aqueous extract of Peperomia pellucida (L.) HBK accelerates fracture healing in Wistar rats. BMC Complement Altern. Med. 17(1), 1-9.

Fraga, C.G., Croft, K.D., Kennedy, D.O., Tomás-Barberán, F.A., 2019. The effects of polyphenols and other bioactives on human health. Food Funct. 10(2), 514-528.

Gomes, P.W.P., Barretto, H., Reis, J.D.E., Muribeca, A., Veloso, A., Albuquerque, C., Teixeira,A., Braamcamp, W., Pamplona, S., Silva, C., Silva, M., 2022. Chemical composition of leaves, stem, and roots of Peperomia pellucida (L.) Kunth. Molecules. 27(6),1847.

Gothai, S., Ganesan, P., Park, S.Y., Fakurazi, S., Choi, D.-K., Arulselvan, P., 2016. Natural phyto-bioactive compounds for the treatment of type 2 diabetes: inflammation as a target. Nutrients. 8(8), 461.

Hidayati, S., 2021. Antidiabetic activity of Peperomia pellucida in streptozotocin-induced diabetic mice. Gale. J. of Pharm. 7(2), 120-130.

Ho, K.L., Tan, C.G., Yong, P.H., Wang, C.W., Lim, S.H., Kuppusamy, U.R., Ngo, C.T.,Massawe, F., Ng, Z.X., 2022a. Extraction of phytochemicals with health benefit from Peperomia pellucida (L.) Kunth through liquid-liquid partitioning. J. Appl. Res. Med.Aromat. Plants. 30, 100392.

Ho, K.L., Yong, P.H., Wang, C.W., Kuppusamy, U.R., Ngo, C.T., Massawe, F., Ng, Z.X.,2022b. Peperomia pellucida (L.) Kunth and eye diseases: A review on phytochemistry, pharmacology and toxicology. J Integr Med. 20(4) 292-304.

Kanedi, M., Mumtazah, D.F., 2021. Ameliorative effect of plant Extract of Suruhan (Peperomia pellucida) on Renal Histological abnormality in Alloxan-Induced Hyperglycemic Mice. South Asian res. j. pharm sci. 3(03), 40-43.

Kartika, I., Insanu, M., Safitri, D., Putri, C., Adnyana, I., 2016. New update: Traditional uses, phytochemical, pharmacological and toxicity review of Peperomia pellucida (L.) Kunth. Pharmacologyonline. 2, 30-43.

Khan, A., Rahman, M., Islam, S., 2008. Antipyretic activity of Peperomia pellucida leaves in rabbit. Turk. J. Biol. 32(1), 37-41.

Lee, S., Sim, K., Wendy, W., Zulhisyam, A., 2016. Peperomia pellucida leaf extract as immunostimulator in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis spp. farming. Vet. World. 9(3), 231.

Nenadis, N., Wang, L.-F., Tsimidou, M., Zhang, H.-Y., 2004. Estimation of scavenging activity of phenolic compounds using the ABTS•+ assay. J. Agric. Food Chem.52(15), 4669-4674.

Ng, Z.X., Than, M.J.Y., Yong, P.H., 2021. Peperomia pellucida (L.) Kunth herbal tea: Effect of fermentation and drying methods on the consumer acceptance, antioxidant and anti-inflammatory activities. Food chem. 344, 128738.

Oyaizu, M., 1986. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. Diet. 44(6),307-315.

Prieto, P., Pineda, M., Aguilar, M., 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 269(2), 337-341.

Riaz, B., Zahoor, M.K., Zahoor, M.A., Majeed, H.N., Javed, I., Ahmad, A., Jabeen, F.,Zulhussnain, M., Sultana, K., 2018. Toxicity, phytochemical composition, and enzyme inhibitory activities of some indigenous weed plant extracts in fruit fly, Drosophila melanogaster. Evid. Based Complementary Altern. Med. 2018, 1-11.

Rogers, S.O., Bendich, A.J., 1988. Recombination in Escherichia coli between cloned ribosomal RNA intergenic spacersfrom Vicia faba: A model for the generation of ribosomal RNA gene heterogeneity in plants. Plant Sci. J. 55(1), 27-31.

Roslida, A., Aini, N., 2009. Evaluation of gastroprotective effects of the ethanolic extract of Peperomia pellucida (L.) Kunth. Pharmacologyonline. 2, 678-686.

Sharma, O.P., Bhat, T.K., 2009. DPPH antioxidant assay revisited. Food Chem. 113(4),1202-1205.

Sheikh, H., Sikder, S., Paul, S.K., Hasan, A.R., Rahaman, M., Kundu, S.P., 2013. Hypoglycemic, anti-inflammatory and analgesic activity of Peperomia pellucida (L.)HBK (piperaceae). Int J Pharm Sci Res. 4(1), 458-463.

Teruna, H.Y., Hendra, R., Almurdani, M., 2022. IAI special edition: α-Glucosidase inhibitory activities of Loranthus ferrugineus and Peperomia pellucida extracts. Pharm. Educ.22(2), 5-8.

Trang, D.T.X., Diem, V.T.N., Hue, Q.T., Phuong, N.T.M., 2012. Investigate the hypoglycemic and antioxidant Efficacy of Noni extract (Morinda citrifolia L.) in diabetes mice. J.of Sci. Can Tho Uni. 23b, 115-124.

Tundis, R., Loizzo, M., Statti, G., Menichini, F., 2007. Inhibitory effects on the digestive enzyme α-amylase of three Salsola species (Chenopodiaceae) in vitro. Pharmazie. 62(6), 473-475.

Tungmunnithum, D., Thongboonyou, A., Pholboon, A., Yangsabai, A., 2018. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines. 5(3), 93.

Usta, A., Güney, İ., Öztürk, M., Selvi, E.K., Mustafa, M., 2020. Toxicological and behavioural potency of different plant extracts on Aedes albopictus (Diptera: Culicidae) and their qualitative phytochemical analysis. Int. J. Mosq. 7(5 Part A), 12-18.

Wei, L.S., Wee, W., Siong, J.Y., Syamsumir, D.F., 2011. Characterization of anticancer,antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract. Acta. Med. Iran. 49(10), 670-674.

Zhang, L., Ravipati, A.S., Koyyalamudi, S.R., Jeong, S.C., Reddy, N., Smith, P.T., Bartlett, J.,Shanmugam, K., Münch, G., Wu, M.J., 2011. Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds.J. Agric. Food Chem. 59(23), 12361-12367.

Zhishen, J., Mengcheng, T., Jianming, W., 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chem. 64(4),555-559.

Zubair, K., Samiya, J., Jalal, U., Mostafizur, R., 2015. In vitro investigation of antdiarrhoeal,antimicrobial and thrombolytic activities of aerial parts of Peperomia pellucida.Pharmacology Online. 3, 5-13.