Effect of fluoxetine on cyclic adenosine monophosphate response to follicle-stimulating hormone in HEK cells https://doi.org/10.12982/VIS.2022.056

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Published: Oct 17, 2022
Keywords:
Adenyl cyclase Adenosine triphosphate AMPK Cyclic AMP Fluoxetine

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Thi Tho Nguyen
Department of Primary Education and Preschool, Quy Nhon University, Binh Dinh 550000, Vietnam
Thi Mong Diep Nguyen
Faculty of Natural Sciences, Quy Nhon University, Binh Dinh 550000, Vietnam

Abstract

Fluoxetine is an antidepressant that acts as a selective serotonin reuptake inhibitor. Fluoxetine has exhibited different mechanisms of action on many different cell lines. In this study, the effect of fluoxetine on cAMP production in human embryonic kidney (HEK) cells under the stimulation of bovine follicle-stimulating hormone were examined. HEK cells were incubated for 60 minutes with fluoxetine at different concentrations (0, 6.25, 12.5, 25 and 50 μM), and then cAMP accumulation, ATP level, and AMPK phosphorylation and cell viability were tested. Our results showed that fluoxetine at 25 and 50 μM attenuated cAMP levels in cells after bFSH stimulation. The depletion of cAMP at this concentration is accompanied by a decrease in ATP energy and an increased capacity to phosphorylate AMPK. As such, this study showed that fluoxetine induces a reduction in cAMP accumulation and that it is associated with the AMPK pathway in HEK cells.

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How to Cite
Tho Nguyen, T. ., & Mong Diep Nguyen, T. . (2022). Effect of fluoxetine on cyclic adenosine monophosphate response to follicle-stimulating hormone in HEK cells: https://doi.org/10.12982/VIS.2022.056. Veterinary Integrative Sciences, 20(3), 731–738. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/259737
Issue
Vol. 20 No. 3 (2022): September-December
Section
Research Articles
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References

Almahariq, M., Mei, F.C., Cheng, X., 2014. Cyclic AMP sensor EPAC proteins and energy homeostasis. Trends Endocrinol. Metab. 25(2), 60-71.

Bariagaber, A.K., Whalen, M.M., 2003. Decreased adenylyl cyclase and cAMP-dependent protein kinase activities inhibit the cytotoxic function of human natural killer cells. Hum. Immunol. 64(9), 866-873.

Boularan, C., Gales, C., 2015. Cardiac cAMP: production, hydrolysis, modulation and detection. Front. Pharmacol. 6, 203.

Carling, D., Zammit, V.A., Hardie, D.G., 1987. A common bicyclic protein kinase cascade inactivates the regulatory enzymes of fatty acid and cholesterol biosynthesis. FEBS.Lett. 223(2), 217-222.

Charles, E., Hammadi, M., Kischel, P., Delcroix, V., Demaurex, N., Castelbou, C., Vacher,A.M., Devin, A., Ducret, T., Nunes, P., 2017. The antidepressant fluoxetine induces necrosis by energy depletion and mitochondrial calcium overload. Oncotarget. 8(2),3181.

Curti, C., Mingattao, F.E., Polizello, A., Galastri, L.O., Uyemura, S.A., Santos, A.C., 1999.Fluoxetine interacts with the lipid bilayer of the inner membrane in isolated rat brain mitochondria, inhibiting electron transport and F1F0-ATPase activity. Mol. Cell Biochem. 199(1), 103-109.

Deák, F., Lasztóczi, B., Pacher, P., Petheö, G.L., Kecskeméti, V., Spät, A., 2000. Inhibition of voltage-gated calcium channels by fluoxetine in rat hippocampal pyramidal cells. Neuropharmacology. 39(6), 1029-1036.

Dong, Y.Y., Pike, A.C., Mackenzie, A., McClenaghan, C., Aryal, P., Dong, L., Quigley, A.,Grieben, M., Goubin, S., Mukhopadhyay, S., 2015. K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac. Science. 347(6227), 1256-1259.

Huang, M., Liang, Y., Chen, H., Xu, B., Chai, C., Xing, P., 2018. The role of fluoxetine in activating Wnt/beta-Catenin signaling and Repressing beta-Amyloid production in an alzheimer mouse model. Front. Aging Neurosci. 10(1), 164.

Hroudová, J., Fišar, Z., 2012. In vitro inhibition of mitochondrial respiratory rate by antidepressants. Toxicol. Lett. 213(3), 345-352.

Kaupp, U.B., Seifert, R., 2002. Cyclic nucleotide-gated ion channels. Physiol. Rev. 82(3),769-824.

Kim, J., Yang, G., Kim, Y., Kim, J., Ha, J., 2016. AMPK activators: mechanisms of action and physiological activities. Exp. Mol. Med. 48(4), e224-e224.

Li, X., Zhu, W., Roh, M.S., Friedman, A.B., Rosborough, K., Jope, R.S., 2004. In vivo regulation of glycogen synthase kinase-3β (GSK3β) by serotonergic activity in mouse brain. Neuropsychopharmacology. 29(8), 1426-1431.

Ma, J., Gao, Y., Jiang, L., Chao, F.-l., Huang, W., Zhou, C.N., Tang, W., Zhang, L., Huang, C.X., Zhang, Y., 2017. Fluoxetine attenuates the impairment of spatial learning ability and prevents neuron loss in middle-aged APPswe/PSEN1dE9 double transgenic Alzheimer's disease mice. Oncotarget. 8(17), 27676.

Muñoz-Llancao, P., Henríquez, D.R., Wilson, C., Bodaleo, F., Boddeke, E.W., Lezoualc'h, F.,Schmidt, M., González-Billault, C., 2015. Exchange protein directly activated by cAMP (EPAC) regulates neuronal polarization through Rap1B. J. Neurosci. 35(32),11315-11329.

Nahon, E., Israelson, A., Abu-Hamad, S., Shoshan-Barmatz, V., 2005. Fluoxetine (Prozac) interaction with the mitochondrial voltage-dependent anion channel and protection against apoptotic cell death. FEBS. Lett. 579(22), 5105-5110.

Nguyen, T.M.D., 2019a. Role of AMPK in mammals reproduction: Specific controls and whole-body energy sensing. C. R. Biol. 342(1-2), 1-6.

Nguyen, T.M.D., Klett, D., Filliatreau, L., Combarnous, Y., 2019b. Inhibition by fluoxetine of LH-stimulated cyclic AMP synthesis in tumor Leydig cells partly involves AMPK activation. PLoS One. 14(6), e0217519.

Nguyen, T.M.D., Klett, D., Combarnous, Y., 2021. Fluoxetine affects cytosolic cAMP, ATP,Ca2+ responses to forskolin, and survival of human ovarian granulosa tumor COV434 cells. Korean J. Physiol. Pharmacol. 25(3), 189-195.

Pancrazio, J.J., Kamatchi, G.L., Roscoe, A.K., Lynch, C., 1998. Inhibition of neuronal Na+ channels by antidepressant drugs. J. Pharmacol. Exp. Ther. 284(1), 208-214.

Pilar‐Cuéllar, F., Vidal, R., Pazos, A., 2012. Subchronic treatment with fluoxetine and ketanserin increases hippocampal brain‐derived neurotrophic factor, β‐catenin and antidepressant‐like effects. Br. J. Pharmacol. 165(4b), 1046-1057.

Sutherland, E.W., Rall, T.W., 1958. Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles. J. Biol. Chem. 232(2), 1077-1091.

Thériault, O., Poulin, H., Beaulieu, J.-M., Chahine, M., 2015. Differential modulation of Nav1. 7 and Nav1. 8 channels by antidepressant drugs. Eur. J. Pharmacol. 764,395-403.

Viollet, B., Horman, S., Leclerc, J., Lantier, L., Foretz, M., Billaud, M., Giri, S., Andreelli, F.,2010. AMPK inhibition in health and disease. Crit. Rev. Biochem. Mol. Biol. 45(4), 276-295.

Wall, E.A., Zavzavadjian, J.R., Chang, M.S., Randhawa, B., Zhu, X., Hsueh, R.C., Liu, J.,Driver, A., Bao, X.R., Sternweis, P.C., 2009. Suppression of LPS-induced TNF-α production in macrophages by cAMP is mediated by PKA-AKAP95-p105. Sci.Signal. 2(75), ra28-ra28.

Wong, D.T., Bymaster, F.P., Engleman, E.A., 1995. Prozac (fluoxetine, Lilly 110140), the first selective serotonin uptake inhibitor and an antidepressant drug: twenty years since its first publication. Life Sci. 57(5), 411-441.