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Fluoxetine is an antidepressant that acts as a selective serotonin reuptake inhibitor. Fluoxetine has exhibited different mechanisms of action on many different cell lines. In this study, the effect of fluoxetine on cAMP production in human embryonic kidney (HEK) cells under the stimulation of bovine follicle-stimulating hormone were examined. HEK cells were incubated for 60 minutes with fluoxetine at different concentrations (0, 6.25, 12.5, 25 and 50 μM), and then cAMP accumulation, ATP level, and AMPK phosphorylation and cell viability were tested. Our results showed that fluoxetine at 25 and 50 μM attenuated cAMP levels in cells after bFSH stimulation. The depletion of cAMP at this concentration is accompanied by a decrease in ATP energy and an increased capacity to phosphorylate AMPK. As such, this study showed that fluoxetine induces a reduction in cAMP accumulation and that it is associated with the AMPK pathway in HEK cells.
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