Clinical haematology, biochemistry profiles and erythrocytic morphometry of confiscated Sunda pangolins (Manis javanica) in the Centre for Rehabilitation and Quarantine of Confiscated Animals, Wildlife and Alien Species, Thailand https://doi.org/10.12982/VIS.2023.043

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Published: May 9, 2023
Keywords:
blood electron microscopy morphology reference interval Sunda pangolin

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Marnoch Yindee AVC, WU
Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand One Health Research Centre, Walailak University, Nakhon Si Thammarat 80160, Thailand
Warisara Thomas
Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand
Worawidh Wajjawalku
Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand
Witsanu Wongsawang
Faculty of Veterinary Science, Mahidol University, Kanchanaburi 71150, Thailand
Nicharee Income
Faculty of Veterinary Science, Mahidol University, Kanchanaburi 71150, Thailand
Wantida Horpiencharoen
School of Veterinary Science, Massey University, Private Bag, 11 222, Palmerston North 4442, New Zealand
Nuttapon Bangkaew
Elephant Kingdom Surin Project by The Zoological Park Organization of Thailand
Worapong Kosaruk
Graduate School of Faculty of Veterinary Medicine, Chiangmai University 50200, Chiangmai, Thailand
Wasinee Thepapichaikul
Graduate School of Faculty of Environment and Resource Studies, Mahidol University, Nakhorn-Pathom 73170, Thailand
Wallaya Manatchaiworakul
National Laboratory Animal Center, Mahidol University, Nakhorn-Pathom 73170, Thailand
Rattapan Pattanarangsan
Panthera Southeast and South Asia, Kanchanaburi 71000, Thailand
Dettachai Ketpun
Veterinary Pathology and Diagnosis Centre, Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand

Abstract

Every year, ten thousand Sunda Pangolins (Manis javanica) are rescued and confiscated from the illegal trade around Southeast Asia, including Thailand. Most of them are usually moribund and need intensive veterinary interventions to recover their health before being released to the wild. However, a lack of reliable haematology and clinical biochemistry references for Thai confiscated Sunda pangolins is a major difficulty for reaching this goal. The aim of this study was to establish a standard panel of haematology and serum biochemistry profiles used for confiscated pangolins in Thailand. Coccygeal venepuncture of 52 confiscated pangolins was performed. Haematology and serum biochemistry data acquisitions were made using automated blood analysers. The lower and upper limits at 90% confidence interval were calculated. The means of all parameters were calculated and then compared with those in two previous studies (p < 0.005). Blood corpuscle morphometry was done manually. All blood corpuscles in females were bigger than males. There were differences of haematologic and biochemical parameters among this study and previous studies in some aspects. Wide scientific discussion was made to explain these variations. A panel of haematology and serum biochemistry profiles for Thai confiscated Sunda pangolin has been established. Veterinarians can refer to all parameters to evaluate the animals’ health and diseases.

Article Details

How to Cite
AVC, WU, M. Y., Thomas, W., Wajjawalku, W., Wongsawang, W., Income, N., Horpiencharoen, W., Bangkaew, N., Kosaruk, W., Thepapichaikul, W., Manatchaiworakul, W., Pattanarangsan, R., & Ketpun, D. (2023). Clinical haematology, biochemistry profiles and erythrocytic morphometry of confiscated Sunda pangolins (Manis javanica) in the Centre for Rehabilitation and Quarantine of Confiscated Animals, Wildlife and Alien Species, Thailand: https://doi.org/10.12982/VIS.2023.043. Veterinary Integrative Sciences, 21(2), 607–622. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/260849
Issue
Vol. 21 No. 2 (2023): May-August
Section
Research Articles
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References

Ahmad, A.A., Samsuddin, S., Oh, P.Y., Martinez-Perez, P., Rasedee, A., 2018. Hematological and serum biochemical parameters of rescued Sunda pangolins (Manis javanica) in Singapore. J. Vet. Med. Sci. 80(12), 1867-1874.

Ahmad, A.A., Sekar, S., Oh, P.Y. Samsuddin, S., 2021. Hematology and serum biochemistry of captive Sunda pangolin (Manis javanica) in Wildlife Reserves Singapore. J. Vet.Med. Sci. 83(2), 309-314.

Carbo, C., Yuki, K., Demers, M., Wagner, D.D., Shimaoka, M., 2013. Isoflurane inhibits neutrophil recruitment in the cutaneous Arthus reaction model. J. Anesth. 27,261-268.

Challender, D.W.S., Thai, N., Van Jones, M., May, L., 2011. Time-budgets and activity patterns of captive Sunda pangolins (Manis javanica). Zoo. Biol. 31, 206–18.

Challender, D., Willcox, D.H.A., Panjang, E., Lim, N., Nash, H., Heinrich, S., Chong, J.,2019. Manis javanica. The IUCN Red List of Threatened Species 2019. Available online: https://dx.doi.org/10.2305/IUCN.UK.2019-3.RLTS.T12763A123584856.en (Accessed on December 5, 2022).

Clark, L., Thai, N.V., Phuong, T.Q., 2008. A long way from home: the health status of Asian Pangolins confiscated from the illegal wildlife trade in Viet Nam. In Proceedings of the Workshop on the Trade and Conservation of Pangolin Native to South and Southeast Asia, Singapore Zoo, Singapore, 30 June - 2 July 2008, pp. 111-131.

Collins, J.D., 1971. The detection of microfilariae using the capillary haemotocrit tube method. Trop. Anim. Health. Prod. 3(1), 23-25.

de Rossi, L.W., Horn, N.A., Buhre, W., Gass, F., Hutschenreuter, G., Rossaint, R., 2002. The effect of isoflurane on neutrophil selectin and beta (2)-integrin activation in vitro.Anesth. Analg. 95(3), 583-587.

Fañanas-Baquero, S., Orman, I., Aparicio, F.B., Bermudez de Miguel, S., Merino, J.G.,Yañez, R., Sainz, Y.F., Sánchez, R., Dessy-Rodríguez, M., Alberquilla, O., Alfaro,D., Zapata, A., Bueren, J.A., Segovia, J.C., Quintana-Bustamante, O., 2021. Natural estrogens enhance the engraftment of human hematopoietic stem and progenitor cells in immunodeficient mice. Haematologica. 106(6), 6591670.

Friedrichs, K.R., Harr, K.E., Freeman, K.P., Szladovits, B., Walton, R.M., Barnhart, K.F.,Blanco-Chavez, J., 2012. ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics. Vet. Clin.Pathol. 41(4), 441-453.

Geffré, A., Concordet, D., Braun, J.P., Trumel, C., 2011. Reference value advisor: a new freeware set of macroinstructions to calculate reference intervals with Microsoft Excel. Vet. Clin. Pathol. 40(1), 107-112.

Heath, M.E., Vanderlip, S.L., 1988. Biology, husbandry, and veterinary care of captive Chinese pangolins (Manis pentadactyla). Zoo. Biol. 7, 293-312.

Hon, M., Thomovsky, E.J., Brooks, A.C., Johnson, P.A., 2020. Cryopreservation of feline red blood cells in liquid nitrogen using glycerol and hydroxyethyl starch. J. Feline.Med. Surg. 22(4), 366-375.

Hussain, A.M., Daniel, R.C.W., 1991. Studies on some aspects of neutrophil functions and uterine defences in Cows during the estrous cycle. Reprod. Domest. Anim. 26(6),290-296.

Ketpun, D., Sailasuta, A., Suwannaphan, T., Bhanpattanakul, S., Pimpin, A., Srituravanich,W., Sripumkhai, W., Jeamsaksiri, W., Piyaviriyakul, P., 2017. The Viability of single cancer cells after exposure to hydrodynamic shear stresses in a spiral microchannel:

a canine cutaneous mast cell tumor model. Micromachines. 9(1), 9.

Kim, M.H., Gorouhi, F., Ramirez, S., Granick, J.L., Byrne, B.A., Soulika, A.M., Simon, S.I.,Isseroff, R.R., 2014. Catecholamine stress alters neutrophil trafficking and impairs wound healing by β2 adrenergic receptor-mediated upregulation of IL-6. J. Invest.134(3), 809-817.

Kim, W.H., Jin, H.S., Ko, J.S., Hahm, T.S., Lee, S.M., Cho, H.S., Kim, M.H., 2011. The effect of anesthetic techniques on neutrophil-to-lymphocyte ratio after laparoscopy-assisted vaginal hysterectomy. Acta. Anaesthesiol. Taiwan. 49(3),83-87.

Kropf, J., Marx, A.M., Hildebrandt, J., Gressner, A.M., 1991. Practical implications of coexistent different technologies in clinical chemical laboratories. Solid phase chemistry and conventional analysis. Eur. J. Clin. Chem. Clin. Biochem. 29(10),675-683.

Lopes-Pereira, C.M., Harun, M., Schmidtova, D., Timane, I., Mabasso, M., Otto, F., Bogin,E., 1996. Use of the dry chemistry "Reflotron" blood analyzer under outdoor-field conditions in veterinary medicine. Eur. J. Clin. Chem. Clin. Biochem. 34(3),231-235.

Moore, G.E., Mahaffey, E.A., Hoenig, M., 1992. Hematologic and serum biochemical effects of long-term administration of anti-inflammatory doses of prednisone in dogs. Am. J.Vet. Res. 53(6), 1033-1037.

Nakada, D., Oguro, H., Levi, B.P., Ryan, N., Kitano, A., Saitoh, Y., Takeichi, M., Wendt, G.R.,Morrison, S.J., 2014. Oestrogen increases haematopoietic stem-cell self-renewal in females and during pregnancy. Nature. 505(7484), 555-558.

Ochi, T., Nishiura, I., Tatsumi, M., Hirano, Y., Yahagi, K., Sakurai, Y., Sudo, Y., Koyama, H.,Hagita, Y., Fujimoto, Y., Kitamura, S., Hashimoto, H., Nakamura, T., Yamada, A.,Tanimoto, M., Nishina, N., 2013. Effects of transport stress on serum alkaline phosphatase activity in beagle dogs. Exp Anim. 62(4), 329-332.

Perera, P., Algewatta, H.R. Karawita, H., 2020. Protocols for recording morphometric measurement of Indian Pangolin (Manis crassicaudata). MethodsX. 7, 101020.

Phillips, S., Wyndham, L., Shaw, J., Walker, S.F., 1988. How accurately does the Reflotron drychemistry system measure plasma total cholesterol levels when used as a community-screening device. Med. J. Aust. 149(3), 122-125.

Pohlin, F., Hooijberg, E.H., Buss, P., Huber, N., Viljoen, F.P., Blackhurst, D., Meyer, L.C.R.,2020. A Comparison of hematological, immunological, and stress responses to capture and transport in wild white rhinoceros bulls (Ceratotherium simum simum)

supplemented with azaperone or midazolam. Front. Vet. Sci. 20(7), 569576.

Prihirunkit, K., Salakij, C., Apibal, S., Narkkong, N.A., 2007. Hematology, cytochemistry and ultrastructure of blood cells in fishing cat (Felis viverrina). J. Vet. Sci. 8(2), 163-168.

Quartuccio, M., Cristarella, S., Medica, P., Fazio, E., Mazzullo, G., Rifici, C., Liotta, L.,Satué, K., 2020. Endometrial cytology during the different phases of the Estrous Cycle in Jennies: new evidences. Animals (Basel). 10(6), 1062.

Rothenberg, M.E., Hogan, S.P., 2006. The eosinophil. Annu. Rev. Immunol. 24, 147-174.

Salaki,j C., Prihirunkit, K., Salakij, J., Narkkong, N.A., Thongthainun, D., 2010a.Characterisation of blood cells in jungle cat, Felis chaus (Carnivora, Felidae). Comp.Clin. Pathol. 20, 319-326.

Salakij, C., Salakij, J., Prihirunkit, K., Apibal, S., Narkkong, N.A., Thongthainun, D., 2008.Hematology, cytochemistry and ultrastructure of blood cells in Clouded leopard (Neofelis nebulosa). J. Anim. Vet. Adv. 7(7), 847-853.

Salakij, C., Salakij, J., Prihirunkit, K., Narkkong, N.A., Pitakkingthong, D., 2010b.Characterization of blood cells in the Leopard Cat (Prionailurus bengalensis). Vet.Clin. Pathol. 39(2), 193-198.

Shepherd, C.R., 2008. Overview of pangolin trade in Southeast Asia. Pangolin capture and trade in Malaysia. In Proceedings of the Workshop on the trade and conservation of pangolins native to South and Southeast Asia, Singapore Zoo, Singapore, 30 June-

July 2008, pp. 6-9.

Szrama, J., Kusza, K., Sobczyński, P., Molnar, Z., Siemionow, M., 2021. The effect of volatile anesthetics on cellular responses in the microcirculation of free tissue transfers. Adv.Interv. Cardiol. 4 (66), 1-6.

Thrall, M.A., Weiser, G., Allison, R.W., Campbell, T.W., 2012. Veterinary hematology and clinical chemistry, 2nd edition. John Wiley & Sons, New Jersey.

Weiser, M.G., Vap, L.M., Thrall, M.A., 2007. Perspectives and advances in in-clinic laboratory diagnostic capabilities: hematology and clinical chemistry. Vet. Clin.North. Am. Small. Anim. Pract. 37(2), 221-36.

Williams, E.S., Thorne, E.T., Kwiatkowski, D.R., Lutz, K., Anderson, S.L., 1992. Comparative vaginal cytology of the estrous cycle of black-footed ferrets (Mustela nigripes), Siberian polecats (M. eversmanni), and domestic ferrets (M. putorius furo).Small. Anim. Pract. 37(2), 221-236.

Yu, J.H., Huong Nguyen, N.D., Lam, H.K., Jimerson, J., Hayek, L.C., Raphael, B.L., Nguyen, V.T., Valitutto, M.T., 2021. Serum biochemistry and select mineral parameters of pre-release Sunda pangolins (Manis Javanica) following rehabilitation in Vietnam. J.Zoo. Wildl. Med. 52(1), 241252.