A histological method for marine invertebrates https://doi.org/10.12982/VIS.2023.020

Article Sidebar

pdf
Published: Feb 20, 2023
Keywords:
Aquatic animal health Histological slides Histopathology Decalcified process Thailand

Main Article Content

Kitiya Kongthong
Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
Natthawut Charoenphon
Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
Narit Thaochan
Agricultural Innovation and Management Division (Pest Management), Faculty of Natural Resources, Prince of Songkla University, 90110 Thailand
Piyakorn Boonyoung
Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla, 90110 Thailand
Atsuo lida
Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
Archig Jeamah
Department of Marine Science and Environment, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang Campus, Sikao, Trang 92150, Thailand
Supapong Imsonpang
Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla, 90110 Thailand
Koraon Wongkamhaeng
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
Piyamat Kongtueng
Central Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
Sinlapachai Senarat Senarat
Department of Marine Science and Environment, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang Campus, Sikao, Trang 92150, Thailand

Abstract

This study aimed to increase knowledge about the structure and health assessment for evaluating histopathology as a biomarker of marine invertebrates, which must have high-quality histologic slides. Unfortunately, the accuracy of this method has been specifically modified for the marine invertebrates in Thailand; we demonstrated a modified method for permanent histological slides, including Xestospongia sp., Halobates hayanus, Amphibalanus amphitrite, and Alpheus sp. (n = 30) individual specimens for each species) from Libong island, Thailand, which was compared to the standard method. Optimization of tissue adapts and troubleshoots typical histological procedures, including sample collection, fixation with 10% Neutral Buffered Formalin at 2-4 °C for 24 hrs., decalcification, dehydration, clearing, embedding sectioning, and examining after staining under the modified procedures each time, which can be accomplished easily in six days. Compared to the standard method, this modified protocol showed the first report. It warranted high-quality slide scans, which are particularly ideal for the tissue, cell, and nuclei-specific features of marine invertebrates.

Article Details

How to Cite
Kongthong, K. ., Charoenphon, N. ., Thaochan, N. ., Boonyoung, P. ., lida, A. ., Jeamah, A. ., Imsonpang, S., Wongkamhaeng, K. ., Kongtueng, P. ., & Senarat, S. S. (2023). A histological method for marine invertebrates: https://doi.org/10.12982/VIS.2023.020. Veterinary Integrative Sciences, 21(1), 251–263. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/261438
Issue
Vol. 21 No. 1 (2023): January-April
Section
Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publishing an article with open access in Veterinary Integrative Sciences leaves the copyright with the author. The article is published under the Creative Commons Attribution License 4.0 (CC-BY 4.0), which allows users to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited.

References

Ahmed, I.E.M., 2015. Lecture notes on : routine histological techniques. University of EI-Imam EI-Mahdi, Kosti, pp. 47.

Alfaro-Montoya, J., 2010. The reproductive conditions of male shrimps, genus Penaeus, sub-genus litopenaeus (open thelyca penaeoid shrimps): a review. Aquaculture.300(1), 1-9.

Al-Sabaawy, H.B., Rahawi, A.M., Al-Mahmood, S.S., 2021. Standard techniques for formalin-fixed paraffin-embedded tissue: a pathologist’s perspective. Iraqi J. Vet. Sci. 35(Supplement I-III), 127–135.

Barbosa, P., Berry, D.L., Kary, C.S., 2014. Dehydrating, clearing, and embedding. In: Barbosa, P., Berry, D.L., Kary, C.S. (Eds.), Insect Histology. Available online: https://onlinelibrary .wiley.com/doi/10.1002/9781118876114.ch3.

Berzins, I.K., Smolowitz, R., Lewbart, G.A., 2021. Diagnostic techniques and sample handling. In: Lewbart, G.A. (Ed), Invertebrate Medicine. Available online: https://doi.org/10.1002 /9781119569831.ch25.

Clarke, G.M., Eidt, S., Sun, L., Mawdsley, G., Zubovits, J.T., Yaffe, M.J., 2007. Wholespecimen histopathology: a method to produce whole-mount breast serial sections for 3-D digital histopathology imaging. Histopathology, 50(2), 232–242.

Copper, J.E., Budgeon, L.R., Foutz, C.A., van Rossum, D.B., Vanselow, D.J., Hubley, M.J.,Cheng, K.C., Mandrell, D.T., Cheng, K.C., 2018. Comparative analysis of fixation and embedding techniques for optimized histological preparation of zebrafish.

Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol. 208, 38–46.

Frank, D., Gruenbaum, B.F., Shelef, I., Zvenigorodsky, V., Benjamin, Y., Shapoval, O., Gal, R., Zlotnik, A., Melamed, I., Boyko, M., 2021. A novel histological technique to assess severity of traumatic brain injury in rodents: comparisons to neuroimaging and neurological outcomes. Front. Neurosci. 15, 733115.

Garcia, T.M., Silva, J.R.F., 2006. Testis and vas deferens morphology of the red-clawed mangrove tree crab (Goniopsis cruentata) (Latreille, 1803). Braz. Arch. Biol.Technol. 49(2), 339–345.

Ghanaati, S., Schlee, M., Webber, M.J., Willershausen, I., Barbeck, M., Balic, E., Görlach,C., Stupp, S.I., Sader, R.A., Kirkpatrick, C.J., 2011. Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic. Biomed.Mater. 6(1), 015010.

Howard, D.W., Lewis, E.J., Keller, B.J., Smith, C.S., 2004. Histological techniques for marine bivalve mollusks and crustaceans. NOAA Technical Memorandum NOS NCCOS 5,Oxford, pp. 218.

Humason, G.L., 1979. Animal tissue technique, 2nd edition. W.H. Freeman and Comp, San francisko.

LaDouceur, E.E.B., 2021. Invertebrate histology. Wiley, Hoboken, NJ.Matthews, M., Varga, Z.M., 2012. Anesthesia and euthanasia in Zebrafish. ILAR J. 53(2),192–204.

Mitchell, D.G., Edgar, A., Martindale, M.Q., 2021. Improved histological fixation of gelatinous marine invertebrates. Front. Zool. 18(1), 29.

Muddana, K., Muppala, J.K., Dorankula, S.R., Maloth, A., Kulkarni, P., Thadudari, D.,2017. Honey and olive oil as bio-friendly substitutes for formalin and xylene in routine histopathology. Indian. J. Dent. Res. 28(3), 286.

Ngu, M.S., Vanselow, D.J., Zaino, C.R., Lin, A.Y., Copper, J.E., Beaton, M.J., Colbourne,J.K., Cheng, K.C., Ang, K.C., 2022. Web-based histology reference atlas for the freshwater crustacean daphnia magna. Available online: https://doi.org/10.1101/2022.03.09.483544

Nuckels, R.J., Gross, J.M., 2007. Histological preparation of embryonic and adult zebrafish eyes. CSH Protoc. 2007, pdb.prot4846.

Senarat, S., Kettratad, J., Plumley, F.G., Wangkulangkul, S., Jiraungkoorskul, W., Boonyoung,P., Poolprasert, P., 2019. Pathological microscopy in liver parenchyma of gray-eel catfish, Plotosus canius, from Ang-Sila area, Chonburi Prov-ince, Thailand. Vet.Integr. Sci., 17, 255-261.

Senarat, S., Kettratad, J., Tipdomrongpong, S., Pengsakul, T., Jiraungkoorskul, W., Boonyoung, P., Huang, S., 2018. Histopathology of kidney and liver in the captive broodstock (Rastrelliger brachysoma) during its juvenile stage. Vet. Integr. Sci., 16,87-93.

Snyder, J.M., Radaelli, E., Goeken, A., Businga, T., Boyden, A.W., Karandikar, N.J.,Gibson-Corley, K.N., 2022. Perfusion with 10% neutral-buffered formalin is equivalent to 4% paraformaldehyde for histopathology and immunohistochemistry in a mouse model of experimental autoimmune encephalomyelitis. Vet. Pathol. 59(3),498–505.

Stewart, A.M., Braubach, O., Spitsbergen, J., Gerlai, R., Kalueff, A.V., 2014. Zebrafish models for translational neuroscience research: from tank to bedside. Trends.Neurosci. 37(5), 264–278.

Stewart, M.J., Stewart, P., Soonklang, N., Linthong, V., Hanna, P.J., Duan, W., Sobhon, P.,2010. Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei. Cell. Tissue. Res. 42(3), 137–150.

Sun, L., Wang, D., Zubovits, J.T., Yaffe, M.J., Clarke, G.M., 2009. An improved processing method for breast whole-mount serial sections for three-dimensional histopathology imaging. Am. J. Clin. Pathol. 131(3), 383-392.

Surgipath Decalcifier Solution - Reagents & Solutions, 2023. Available online: https://shop.leicabiosystems.com/us/histology-consumables/reagents-solutions/pid-decalcifiersystem Swamy, S.R.G., 2015. Bio-friendly alternatives for xylene – carrot oil, olive oil, pine oil, rose oil. J. Clin. Diagn. Res. 9(11), 1-18.

Wilson, J.M., Bunte, R.M., Carty, A., 2009. Evaluation of rapid cooling and tricaine methanesulfonate (MS222) as methods of euthanasia in zebrafish (Danio rerio). J.Am. Assoc. Lab. Anim. Sci. 48(6), 785–789.

Zarella, M.D., Bowman, D., Aeffner, F., Farahani, N., Xthona, A., Absar, S.F., Parwani, A.,Bui, M., Hartman, D.J., 2019. A practical guide to whole slide imaging: A white paper from the digital pathology association. Arch. Pathol. Lab. Med. 143(2),222-234.

Zhang, X., Zhou, X., Yue, Z., Li, Z., 2010. Enhancing teaching effectiveness in animal histology by taking reproductive system as an example. Biol. Reprod. 83(1),237–237.