Human rapid influenza diagnostic test: efficacy and significance for using in animal

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Duanghathai Saipinta
Phongsakorn Chuammitri
Satoshi Ohkura
Banlang Luangwaranan
Pakpoom Tadee


Rapid influenza diagnostic tests (RIDTs) could considerably assist in clinical management resolving outbreaks of diseases in humans and animals. The RIDT for animals is rarely available and considerably expensive, especially in Thailand. In this study, therefore, the objective of this study was to determine the capacity of a commercial human RIDT “QuickNavi™Flu2”, an RIDT for influenza viruses A and B, to detect influenza viruses A antigen including 2 inactivated swine influenza viruses: H1N1 and H3N2, canine parainfluenza virus, and feline calicivirus. In addition, the efficacy of QuickNavi™Flu2 was compared with the real-time polymerase chain reaction assay (real-time rt-PCR). Both of SIV were used as stock solutions, and were serially diluted 6 concentrations before testing. The stock and diluted solutions of both SIV, specimens of canine parainfluenza virus, feline calicivirus and 96 unknown samples were tested by QuickNavi™Flu2 according to the guideline. Then all of samples were repeated testing with real-time rt-PCR assay. Results showed that Quick Navi-Flu could not give positive results for dilutions lower than 1:10 and 1:100, respectively. The sensitivity and specificity values of QuickNavi™Flu2 test in comparison to real-time rt-PCR assay for influenza A virus were 36.1% and 100%, respectively. In conclusion, a human RIDT can detect swine influenza H1N1 and H3N2 in high viral concentration, and appeared to be a useful tool for screening in animals when the viral load is sufficient.

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Saipinta, D. ., Chuammitri, P., Ohkura, S. ., Luangwaranan, B. ., & Tadee, P. . (2023). Human rapid influenza diagnostic test: efficacy and significance for using in animal: Veterinary Integrative Sciences, 21(2), 265–271. Retrieved from
Research Articles


Andresen, D.N., Kesson, A.M., 2010. High sensitivity of a rapid immunochromatographic test for detection of influenza a virus 2009 H1N1 in nasopharyngeal aspirates from young children. J. Clin. Microbiol. 48(7), 2658-2659.

Centers for Disease Control and Prevention. Rapid diagnostic testing for influenza information for health care professionals Available online: diagnosis/rapidclin.htm. (Accessed on March 1, 2010).

Charoenvisal, N., Keawcharoen, J., Sreta, D., Tantawet, S., Jittimanee, S., Arunorat, J.,Amonsin. A., Thanawongnuwech, R., 2013. Experimental infection with a Thai reassortant swine influenza virus of pandemic H1N1 origin induced disease. Virol. J.10(1), 88.

Chauhan, N., Narang, J., Pundir, S., Singh, S., Pundir, C.S., 2013. Laboratory diagnosis of swine flu: a review. Artif. Cells. Nanomed. Biotechnol. 41(3), 189–195.

Chidlow, G., Harnett, G., Williams, S., Levy, A., Speers, D., Smith, D.W., 2010. Duplex real-time reverse transcriptase PCR assays for rapid detection and identification of pandemic (H1N1) 2009 and seasonal influenza A/H1, A/H3, and B viruses. J. Clin.Microbiol. 48(3), 862-866.

Chua, T-H., Ellis, T.M., Wong, C.W., Guan, Y., Ge, S.X., Peng, G., Lamichhane, C., Maliadis, C., Tan, S., Selleck, P., 2007. Performance evaluation of five detection tests for avian influenza antigen with various avian samples. Avian. Dis. 51(1), 96–105.

Crum-Cianflone, N.F., Blair, P.J., Faix, D., Arnold, J., Echols, S., Sherman, S.S., Tueller, J.E.,Warkentien, T., Sanguineti, G., Bavaro, M., 2009. Clinical and epidemiologic characteristics of an outbreak of novel H1N1 (swine origin) influenza A virus among United States military beneficiaries. Clin. Infect. Dis. 49(12), 1801–1810.

Decorte, I., Steensels, M., Lambrecht, B., Cay, A.B., De Regge, N., 2015. Detection and isolation of swine influenza a virus in spiked oral fluid and samples from individually housed, experimentally infected pigs: Potential role of porcine oral fluid in active influenza a virus surveillance in swine. PLoS One. 10(10), e0139586.

Drexler, J.F., Helmer, A., Kirberg, H., Reber, U., Panning, M., Müller, M., Höfling, K., Matz,B., Drosten, C., Eis-Hübinger, A.M., 2009. Poor clinical sensitivity of rapid antigen test for influenza a pandemic (H1N1) 2009 virus. Emerg. Infect. Dis. 15(10), 1662-1664.

Green, D.A., StGeorge, K., 2018. Rapid antigen tests for influenza: Rationale and significance of the FDA reclassification. J. Clin. Microbiol. 56(10), e00711-18.

Jernigan, D.B., Lindstrom, S.L., Johnson, J.R., Miller, J.D., Hoelscher, M., Humes, R.,Shively, R., Brammer, L., Burke, S.A., Villanueva, J.M., Balish, A., Uyeki, T.,Mustaquim, D., Bishop, A., Handsfield, J.H., Astles, R., Xu, X., Klimov, A.I.,Cox, N.J., Shaw, M.W., 2011. Detecting 2009 pandemic influenza a (H1N1) virus infection: availability of diagnostic testing led to rapid pandemic response. Clin.Infect. Dis. 52(Suppl 1), S36-43.

Kang, X., Jiang, T., Li, Y., Lin, F., Liu, H., Chang, G., Zhu, Q., Qin, E., Qin, C., Yang,Y., 2010. A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus. Virol. J. 7(1), 113.

Kaplan, B.S., Webby, R.J., 2013. The avian and mammalian host range of highly pathogenic avian H5N1 influenza. Virus. Res. 178(1), 3–11.

Louie, J.K., Guevara, H., Boston, E., Dahlke, M., Nevarez, M., Kong, T., Schechter, R.,Glaser, C.A., Schnurr, D.P., 2010. Rapid influenza antigen test for diagnosis of pandemic (H1N1) 2009. Emerg. Infect. Dis. 16(5), 824–826.

Pabbaraju, K., Wong, S., Wong, A.A., Appleyard, G.D., Chui, L., Pang, X.L., Yanow, S.K.,Fonseca, K., Lee, B.E., Fox, J.D., Preiksaitis, J.K., 2009. Design and validation of real-time reverse transcription-PCR assays for detection of pandemic (H1N1) 2009 virus. J. Clin. Microbiol. 47(11), 3454-3460.

Peaper, D.R., Landry, M.L., 2014. Rapid diagnosis of influenza: state of the art. Clin. Lab.Med. 34(2), 365–385.

Piralla, A., Daleno, C., Pariani, E., Conaldi, P., Esposito, S., Zanetti, A., Baldanti, F., 2013.Virtual quantification of influenza A virus load by real-time RT-PCR. J. Clin. Virol.56(1), 65–68.

Richard, M., Fouchier, R.A., 2016. Influenza A virus transmission via respiratory aerosols or droplets as it relates to pandemic potential. FEMS Microbiol. Rev. 40(1), 68–85.

Spackman, E., Senne, D.A., Myers, T.J., Bulaga, L.L., Garber, L.P., Perdue, M.L., Lohman,K., Daum, L.T., Suarez, D.L., 2002. Development of a real-time reverse transcriptase pcr assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J. Clin. Microbiol. 40(9), 3256-3260.

Sreta, D., Kedkovid, R., Tuamsang, S., Kitikoon, P., Thanawongnuwech, R., 2009.Pathogenesis of swine influenza virus (Thai isolates) in weanling pigs: an experimental trial. Virol. J. 6(1), 34.

Takemae, N., Parchariyanon, S., Damrongwatanapokin, S., Uchida, Y., Ruttanapumma, R.,Watanabe, C., Yamaguchi, S., Saito, T., 2008. Genetic diversity of swine influenza viruses isolated from pigs during 2000 to 2005 in Thailand. Influenza. Other. Respir.Viruses. 2(5), 181-189.