Live yeasts as a non-hormonal alternative to improve the performance of dairy cows

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Jefferson R. Gandra
Juliane Damiani
Tiago A. Del Valle
Caio S. Takiya
Euclides R. Oliveira
Rafael H. T. B. Goes
Cibeli A. Pedrini
Erika R. S. Gandra


The present study aimed to evaluate live yeast dietary supply instead of rbST application in lactating dairy cows. Thirty-five Holstein cows (37.1 ± 7.8 kg/d of milk yield and 524 ± 27 kg of body weight; mean ± SD) were used in a completely random trial to evaluate: 1) rbST: animals treated with 500 mg of rbST every 14 d; and 2) Yeast: animals fed diets containing 40 g/d of live yeast. The trial lasted for five subsequent 14-d periods. Treatments showed no effects on cows’ feed intake. Yeast reduced large and small particles selection indexes compared, feed residue and starch in feces compared to rbST. Cows treated with rbST had increased glucose, triglycerides, and AST, and decreased cholesterol and urea serum concentration. In addition, yeast reduced somatic cells count and increased milk yield and cows’ production efficiency. Although treatments showed no effects on milk chemical composition, yeast increased saturated to unsaturated fatty acids ratio. Yeast increased saturated and short-chain fatty acids, whereas reduced unsaturated and cis-9 trans-11 C18:2 FA content in milk fat. Thus, yeast could be used instead of rbST to improve milk yield and the productive efficiency of cows in a short-term evaluation

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R. Gandra, J. ., Damiani, J. ., A. Del Valle, T. ., S. Takiya, C. ., R. Oliveira, E. ., H. T. B. Goes, R. ., A. Pedrini, C. ., & R. S. Gandra, E. . (2023). Live yeasts as a non-hormonal alternative to improve the performance of dairy cows: Veterinary Integrative Sciences, 21(2), 397–409. Retrieved from
Research Articles


Bauman, D.E., Harvatine, K.J., Lock, A.L., 2011. Nutrigenomics, rumen-derived bioactive fatty acids, and the regulation of milk fat synthesis. Annu. Rev. Nutr. 31, 299-319.

Dell'Orto, V., Savoini, G., Salimei, E., Cattaneo, D., Secchi, C., Rosi, F., 1993. Effects of recombinant bovine somatotropin (rbst) on productive and physiological parameters related to dairy cow welfare. Livest. Prod. Sci. 36(1), 71-75.

Dennis, T.S., Hu, W., Suarez-Mena, F.X., Hill, T.M., Quigley, J.D., Schlotterbeck, R.L., 2017. Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves. J. Dairy Sci. 100(8), 6266-6271.

Dias, A.L.G., Freitas, J.A., Micai, B., Azevedo, R.A., Greco, L.F., Santos, J.E.P., 2018a. Effect of supplemental yeast culture and dietary starch content on rumen fermentation and digestion in dairy cows. J. Dairy Sci. 101(1), 201-221.

Dias, J.D.L., Silva, R.B., Fernandes, T., Barbosa, E.F., Graças, L.E.C., Araujo, R.C., Pereira, R.A.N., Pereira, M.N., 2018b. Yeast culture increased plasma niacin concentration, evaporative heat loss, and feed efficiency of dairy cows in a hot environment. J. Dairy Sci. 101(7), 5924-5936.

European Food Safety Authority, 2015. EFSA's assistance for the 2015 codex committee on residues of veterinary drugs in food (CCRVDF) in relation to rbST. EFSA. 12(6),828E.

Food and Agriculture Organization of the United Nations, 2014. Residue evaluation of certain veterinary drugs. In 78th meeting 2013 of the Joint FAO/WHO Expert Committee on Food Additives, Geneva, 5-14 November 2013. Available online: /i3745e.pdf (Accessed on January 18, 2017).

Gregorini, P., Waghorn, G.C., Kuhn-Sherlock, B., Romera, A.J., Macdonald, K.A., 2015. Short communication: Grazing pattern of dairy cows that were selected for divergent residual feed intake as calves. J. Dairy Sci. 98(9), 6486-6491.

Moallem, U., Lehrer, H., Livshitz, L., Zachut, M., Yakoby, S., 2009. The effects of live yeast supplementation to dairy cows during the hot season on production, feed efficiency, and digestibility. J. Dairy Sci. 92(1), 343-351.

Moya-Camarena, S.Y., Vanden Heuvel, J.P., Blanchard, S.G., Leesnitzer, L.A., Belury, M.A., 1999. Conjugated linoleic acid is a potent naturally occurring ligand and activator of pparalpha. J. Lipid Res. 40(8), 1426-1433.

Newbold, C.J., Wallace, R.J., McIntosh, F.M., 1996. Mode of action of the yeast saccharomyces cerevisiae as a feed additive for ruminants. Br. J. Nutr. 76(2),249-261.

Perdomo, M.C., Marsola, R.S., Favoreto, M.G., Adesogan, A., Staples, C.R., Santos, J.E.P.,2020. Effects of feeding live yeast at 2 dosages on performance and feeding behavior of dairy cows under heat stress. J. Dairy Sci. 103(1), 325-339.

Singer, R.S., Ruegg, P.L., Bauman, D.E., 2017. Quantitative risk assessment of antimicrobial-resistant foodborne infections in humans due to recombinant bovine somatotropin usage in dairy cows. J. Food. Prot. 80(7), 1099-1116.

St-Pierre, N.R., Milliken, G.A., Bauman, D.E., Collier, R.J., Hogan, J.S., Shearer, J.K., Smith,K.L., Thatcher, W.W., 2014. Meta-analysis of the effects of sometribove zinc suspension on the production and health of lactating dairy cows. J. Am. Vet. Med.Assoc. 245(5), 550-564.

Westerveld, A., 2017. Innovation is critical. Dairy. Mail. 24(5), 87-91. Available online: