Supplementation of different sources of selenium on laying performance, egg quality traits, and shell calcification expressions in 50 weeks Lohmann Brown layer hen reproductive tract

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Nur Izzah Mohd Hemly
Nurafiqah Najwa Zainuddin
Aliyu Ibrahim Muhammad
Loh Teck Chwen
Anjas Asmara Samsudin


Massive consumption and exportation of eggs in Malaysia are possible by maintaining and improving layer productive performances and egg quality through antioxidant supplementation such as selenium. The bioavailability of organic selenium in tissues resulted in a preference for organic selenium in poultry diets. The present study assesses the effects of feeding 50 weeks layer hens with sodium selenite, selenised yeast, and Stenotrophomonas maltophilia, ADS18 as a source of selenium and vitamin E on laying integrity, egg qualities, and reproductive gene expressions. The four different treatments (diets) used in the experiment were tagged as follows: Control: a basal diet containing 100 mg/kg vitamin E, SS: basal diet plus 0.3 mg/kg sodium selenite, Se-yeast: basal diet plus 0.3 mg/kg selenised yeast, and VADS18: basal diet plus 0.3 mg/kg ADS18. Productive performances were analysed throughout a 90-days of production. Eggs were collected biweekly, and egg qualities were measured. The uterine and magnum tissues were examined for the genes ovocleidin and ovocalyxin; OCX32, OCX36, OC17 and OC116. The results showed that Se-yeast significantly (P < 0.05) increased laying integrity, while VADS18 supplementation significantly (P < 0.05) increased egg weight. Supplementation of different selenium sources significantly (P < 0.05) improved egg production and quality. Additionally, uterine gene expression was significantly (P < 0.05) increased by VADS18 supplementation. Therefore, adding organic selenium to the diet maintained various laying performance indicators, egg qualities, and upregulated gene expression.  Further evaluations are required to determine the optimum inclusion level of ADS18 poultry.

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Hemly, N. I. M., Zainuddin , N. N., Muhammad , A. I. ., Loh Teck Chwen, & Samsudin, A. A. (2023). Supplementation of different sources of selenium on laying performance, egg quality traits, and shell calcification expressions in 50 weeks Lohmann Brown layer hen reproductive tract: Veterinary Integrative Sciences, 21(3), 677–692. Retrieved from
Research Articles
Author Biography

Nur Izzah Mohd Hemly, Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia


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