Effects of organic acid and garlic powder supplementations in the diet on growth and intestinal microflora of the exotic Tam Hoang chickens https://doi.org/10.12982/VIS.2023.039

Main Article Content

Nguyen Thi Thuy
Le Thanh Phuong
Nguyen Cong Ha


A study was carried out to evaluate the effect of diet’s organic acid (OG) and garlic powder (GP) on the growth, carcass quality, and intestinal microflora of Tam Hoang chickens. The experiment consisted of three treatments and four replications in a completely randomized design; each replication was a pen (20 chickens/pen), and males and females were raised separately. The treatmentswere:1/Cont: Basal diet (BD) no supplementation; 2/OG: BD + 1g Poulacid /kg feed; 3/GP: BD + 1 g garlic powder/kg feed. Results showed that chickens fed Cont (1451 g /head) had lower final live weight than those having OG (1568 g/head) and GP (1521 g/head/day). So, the average daily gain (ADG) of chickens in Cont (16.8 g/head/day) and GP (17.7 g/head/day) were lower than in OG (18.2 g/head/day). Average daily feed intake (ADFI) was similar in all treatments. Therefore, the feed conversion ratio (FCR) of chickens fed OG (3.04 kg feed/kg gain) was better than that in Cont (3.32 kg feed/kg gain). The density of Salmonella bacteria was not detectable in chicken feces at the 4th week age but became positive in Cont and GP treatments at the 10th week age. Supplementation of OG and GP reduced E. coli and Clostridium perfringens in the feces compared to control chickens at the 4th and 10th week age. In conclusion, adding OG or GP at the same dose (1g/kg feed) in Tam Hoang chicken diets improved ADG, FCR, and reduced E. coli and Clostridium perfringens densities in the feces compared to control chickens

Article Details

How to Cite
Nguyen Thi Thuy, Le Thanh Phuong, & Nguyen Cong Ha. (2023). Effects of organic acid and garlic powder supplementations in the diet on growth and intestinal microflora of the exotic Tam Hoang chickens: https://doi.org/10.12982/VIS.2023.039. Veterinary Integrative Sciences, 21(2), 557–565. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/262821
Research Articles


Adil, S., Banday, M., Bhat, G., Qureshi, S., Wani, S., 2011a. Effect of supplemental organic acids on growth performance and gut microbial population of broiler chicken. Livest. Res. Rur. Dev. 23(1), 1-8.

Adil, S., Banday, T., Ahad Bhat, G., Salahuddin, M., Raquib, M., Shanaz, S., 2011b. Response of broiler chicken to dietary supplementation of organic acids. J. Cent. Eur. Agric.12(3), 498-508.

Ali, F., Zahran, D., 2010. Effect of growth enhancers on quality of chicken meat during cold storage. Adv. J. Food. Sci. Technol. 2(4), 219-226.

Amagase, H., Petesch, B.L., Matsuura, H., Kasuga, S., Itakura, Y., 2001. Intake of garlic and its bioactive components. J. Nutr. 131(3), 955S-962S.

Association of Official Analytical Chemists, 1990. Official methods of analysis, 5th edition.AOAC, Arlington, Virginia.

Bedford, M., 2000. Removal of antibiotic growth promoters from poultry diets: Implications and strategies to minimise subsequent problems. Worlds Poult Sci J. 56(4), 347-365.

Diaz-Sanchez, S., D’Souza, D., Biswas, D., Hanning I., 2015. Botanical alternatives to antibiotics for use in organic poultry production. Poult. Sci. 94(6), 1419-1430.

Fatufe, A., Matanmi, I., 2011. Effect of probiotics, organic acids or their mix on the growth performance of starting cockerels. Arch. Zootec. 60(229), 149-152.

Goodarzi, M., Nanekarani, S., Landy, N., 2014. Effect of dietary supplementation with onion (Allium cepa L.) on performance, carcass traits and intestinal microflora composition in broiler chickens. Asian. Pac. J. Trop. Dis. 4, S297-S301.

General Statistics Office of Vietnam, 2021. Statistic of livestock heads and production of Viet Nam Animal husbandry. GSO, Vietnam.

Gull, I., Saeed, M., Shaukat, H., Aslam, S.M., Samra, Z.Q., Athar, A.M., 2012. Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Ann. Clin. Microbiol. Antimicrob. 11(1), 1-6.

Jamroz, D., Orda, J., Kamel, C., Wiliczkiewicz, A., Wertelecki, T., Skorupińska, J., 2003. The influence of phytogenic extracts on performance, nutrient digestibility, carcass characteristics, and gut microbial status in broiler chickens. J. Anim. Feed Sci. 12(3),583-596.

Moutzouris, J.P., Che, W., Ramsay, E.E., Manetsch, M., Alkhouri, H., Bjorkman, A.M.,Schuster, F., Ge, Q., Ammit, A.J., 2010. Proteasomal inhibition upregulates the endogenous MAPK deactivator MKP-1 in human airway smooth muscle: mechanism of action and effect on cytokine secretion. Biochim. Biophys. Acta. Mol. Cell Res. 1803(3), 416-423.

Muzaffer, D., Ferda, O., Kemal, C., 2003. Effect of dietary probiotic, organic acid and antibiotic supplementation to diets on broiler performance and carcass yield. Pak. J. Nutr. 2(2),89-91.

Olobatoke, R., Mulugeta, S., 2011. Effect of dietary garlic powder on layer performance, fecal bacterial load, and egg quality. Poult. Sci. 90(3), 665-670.

Panpatil, V.V., Tattari, S., Kota, N., Nimgulkar, C., Polasa, K., 2013. In vitro evaluation on antioxidant and antimicrobial activity of spice extracts of ginger, turmeric and garlic. J. Pharmacogn. Phytochem. 2(3), 143-148.

TCVN 7924, 2008. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli - Part 2: Colony-count technique at 44 oC using 5-bromo-4-chloro-3-indolyl β-D-glucuronide (ISO 16649-2 : 2001). Vietnam Standard and Quality Institute, Vietnam.

TCVN 4991, 2005. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony count technique (ISO 7937 : 2004). Vietnam Standard and Quality Institute, Vietnam.

TCVN 10780, 2017. Microbiology of the food chain - Horizontal method for the detection,enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp (ISO 6579-1:2017). Vietnam Standard and Quality Institute, Vietnam.

Willey, J.M., Sherwood, L., Woolverton, C.J., 2009. Prescott’s principles of microbiology.McGraw-Hill, New York.