Molecular characterization and identification of African Swine Fever virus isolates from emerging cases of infection in Central Luzon, Philippines https://doi.org/10.12982/VIS.2024.009

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Published: Sep 12, 2023
Keywords:
African Swine Fever Central Luzon Philippines Emerging ASF P72 Molecular characterization

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Dr. Gemerlyn G. Garcia
Veterinary Microbiology Laboratory, Department of Pathobiology, College of Veterinary Science and Medicine Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
Dr. Madison Munar
Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
Dr. Marvin Bryan Salinas
Department of Morphophysiology and Pharmacology, College of Veterinary Science and Medicine Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
Mr. Gener D. Gregorio
Veterinary Microbiology Laboratory, Department of Pathobiology, College of Veterinary Science and Medicine Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
Dr. Milagros R. Mananggit
Regional Animal Disease Diagnostic Laboratory, Region 3, Department of Agriculture, Tarlac City, Tarlac, Philippines

Abstract

A study that evaluated the molecular characteristics to confirm the identity of the African Swine Fever (ASF) pathogen from emerging cases of infection in Central Luzon, Philippines was initiated. The study involved the collection of 125 blood samples from pigs during the four-month duration of the study when clinical signs of ASF was prevalent in the four out of seven provinces of Central Luzon, Philippines. This work included the amplification of the p72 gene in DNA samples through PCR and confirmation of the identity of the ASF pathogen through DNA sequencing. The DNA sequences of the p72 gene of the ASF pathogen are made up of 412 nucleotides and used as a basis in identifying the Philippine ASF pathogens as ASF virus PH/CL 4/2022, ASF virus PH/CL 3/2022, ASF virus PH/CL 2/2022 and ASF virus PH/CL 1/2022. Philippines ASF virus isolates showed 100% sequence similarity with sequences reported from Malaysia ASF isolate MVZT218/2021, Vietnam ASF isolate D/ASFV/VN/DN-VC/2019, and India ASF isolate ASF/MZ/IND/3 based on BLASTN homology search in the NCBI database. The results of the study revealed close phylogenetic relationships among ASF isolates from Vietnam, Malaysia, India and the Philippines.

Article Details

How to Cite
Garcia, G., Munar, M. ., Salinas, M. B. ., Gregorio, G., & Mananggit, M. . (2023). Molecular characterization and identification of African Swine Fever virus isolates from emerging cases of infection in Central Luzon, Philippines: https://doi.org/10.12982/VIS.2024.009. Veterinary Integrative Sciences, 22(1), 111–119. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/262864
Issue
Vol. 22 No. 1 (2024): January-April
Section
Research Articles
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Author Biographies

Dr. Madison Munar, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

Associate Professor, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

Dr. Marvin Bryan Salinas, Department of Morphophysiology and Pharmacology, College of Veterinary Science and Medicine Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

Instructor, Department of Morphophysiology and Pharmacology, College of Veterinary Science and Medicine Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

Mr. Gener D. Gregorio, Veterinary Microbiology Laboratory, Department of Pathobiology, College of Veterinary Science and Medicine Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

Research Assistant, Veterinary Microbiology Laboratory, Department of Pathobiology, College of Veterinary Science and Medicine Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

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