Genotypic Characterization of Marek’s Disease Virus from Clinically Suspected Layer in Basrah, South of Iraq https://doi.org/10.12982/VIS.2024.025
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Abstract
A reemerging viral disease known as Marek's disease affects chickens and has a devastating financial impact on the global poultry sector. The emergence of pathogenic strains must be continuously monitored using molecular approaches of Marek’s disease and to adevise to use appropriate vaccination program with implement bio-security. This study was continued from May 2021 to August2021. Clinical symptoms and postmortem lesions were used to identify MD, and PCR testing for the Meq gene provided further confirmation. The meq gene's phylogenetic analysis was also performed, and such test revealed clustering of the our Iraqi field strains with Euroasian cluster, where the genetic characteristics of the present study MD strains were similar to those of Chinese and European strains
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References
Abd-Ellatieff, H.A., Abou Rawash, A.A., Ellakany, H.F., Goda, W.M., Suzuki, T., Yanai, T.,2018. Molecular characterization and phylogenetic analysis of a virulent marek’s disease virus field strain in broiler chickens in Japan. Avian. Pathol. 47(1), 47-57.
Abdallah, F., Hassanin, O., Attar, E., Ali, H., Megahed, M., Nair, V., 2018. Marek’s disease virus in Egypt: historical overview and current research based on the major MDVEncoded Oncogene Meq. Hosts and Viruses. 5(3), 35-43.
Adedeji, A.J., Akanbi, O.B., Luka, P.D., Abdu, P., 2019. Natural outbreak of Marek’s disease in indigenous chicken and Japanese quail (Coturnix coturnix japonica) in Jos,Plateau State, Nigeria. Open. Vet. J. 9(2), 151-156.
Arulmozhi, A., Saravanan, S., Mohan, B., Balasubramaniam, G.A., 2011. Marek’s disease in vaccinated poultry flocks in and around Namakkal region of Tamil Nadu. Indian J. Vet. Pathol. 35, 45-47.
Boodhoo, N., Gurung, A., Sharif, S., Behboudi, S., 2016. Marek’s disease in chickens: a review with focus on immunology. Vet. Res. 47(1), 1-19.
Demeke, B., Jenberie, S., Tesfaye, B., Ayelet, G., Yami, M., Lamien, C.E., Gelaye, E., 2017. Investigation of Marek’s disease virus from chickens in central Ethiopia. Trop.Anim. Health. Prod. 49(2), 403-408.
Dunn, J.R., Gimeno, I.M., 2013. Current status of Marek’s disease in the United States and worldwide based on a questionnaire survey. Avian. Dis. 57(2s1), 483-490.
Fenner, F.J., Stephen, W., Richard, A., Ronald, P., Donald, P., Michael, D., Colin, R., Linda,J., David, E., 2011. Veterinary virology, 4th edition. Academic Press, London, pp.180-193.
Lounas, A., Besbaci, M., Akkou, M., Tali, O., 2021. Occurrence of Marek’s disease in vaccinated Algerian broiler breeder flocks: a histopathological survey. Vet. World. 14(11), 3021.
Gall, S., Kőrösi, L., Cortes, A.L., Delvecchio, A., Prandini, F., Mitsch, P., Gimeno, I.M.,2018. Use of real-time PCR to rule out Marek’s disease in the diagnosis of peripheral neuropathy. Avian. Pathol. 47(4), 427-433.
Jarosinski, K.W., Margulis, N.G., Kamil, J.P., Spatz, S.J., Nair, V.K., Osterrieder, N., 2007. Horizontal transmission of Marek’s disease virus requires US2, the UL13 protein kinase, and gC. Virol. J. 81(19), 10575-10587.
Lee, L.F., Kreager, K., Heidari, M., Zhang, H., Lupiani, B., Reddy, S.M., Fadly, A., 2013. Properties of a meq-deleted rMd5 Marek’s disease vaccine: protection against virulent MDV challenge and induction of lymphoid organ atrophy are simultaneously attenuated by serial passage in vitro. Avian. Dis. 57(2s1), 491-497.
Nair, V.,2018. Spotlight on avian pathology: Marek’s disease. Avian. Pathol. 47(5), 440–442.
OIE, 2010. Marek’s Disease. OIE manual of diagnostic tests and vaccines of terrestrial animals. Office International des Epizootics (OIE), Paris, France, pp. pp. 1-11.
Padhi, A., Parcells, M.S., 2016. Positive selection drives rapid evolution of the meq oncogene of Marek’s disease virus. PLoS One. 11(9), e0162180.
Shamblin, C.E., Greene, N., Arumugaswami, V., Dienglewicz, R.L., Parcells, M.S., 2004. Comparative analysis of Marek’s disease virus (MDV) glycoprotein-, lytic antigen pp38-and transformation antigen Meq-encoding genes: association of meq mutations
with MDVs of high virulence. Vet. Microbiol. 102(3-4), 147-167.
Sullivan, C.S., Grundhoff, A.T., Tevethia, S., Pipas, J.M., Ganem, D., 2005. SV40-encoded microRNAs regulate viral gene expression and reduce susceptibility to cytotoxic T cells. Nature. 435(7042), 682.
Trimpert, J., Groenke, N., Jenckel, M., He, S., Kunec, D., Szpara, M.L., McMahon, D.P.,2017. A phylogenomic analysis of Marek’s disease virus reveals independent paths to virulence in Eurasia and North America. Evol. Appl. 10(10),1091-1101.
Trang, H.N., Chien, N.T.P., Thu, H.T.V., 2022. Prevalence of Marek’s disease virus in unvaccinated healthy backyard chickens in Cantho city, Vietnam and genetic characterization of meq gene. Vet. Integr. Sci. 20(3), 709-718.
Wajid, J., Margaret, E., Katrin, G., Stephen, W., 2013. Prevalence of Marek’s Disease Virus in different chicken populations in Iraq and indicative virulence based on sequence variation in the EcoRI-Q (meq) Gene. Avian. Dis. 57(2s1), 562–568.
Witter, R.L., Gimeno, I.M., Pandiri, A.R., Fadly, A.M., 2010. Tumor diagnosis manual: the differential diagnosis of lymphoid and myeloid tumors in the chicken. American Association of Avian Pathologists, Florida, pp. 7-84.