Comparative morphology of blood cells of Ambassis nalua, A. vachelli, and Inegocia japonica https://doi.org/10.12982/VIS.2024.002
Article Sidebar
Main Article Content
Abstract
Hematological investigation is an essential tool for monitoring fish health. This research compared morphometric traits and blood cell characteristics between two representative pelagic fish species (Ambassis nalua and A. vachelli) and one benthic species (Inegocia japonica) collected from seagrass meadows off Libong Island in Thailand, where they coexist with human activity. Blood samples were collected and prepared using the smear technique. The erythrocytes of all observed specimens had elliptical or oval shapes. The benthic fish I. japonica significantly outperformed both pelagic fish in terms of the mean size of many erythrocyte features. However, more erythrocyte nuclear abnormalities were found in I. japonica, along with A. nalua. Lymphocytes made up the highest proportion of leukocytes in I. japonica, followed by neutrophils. The morphometric erythrocyte data of I. japonica possibly indicated the greater oxygen requirement of fish living in demersal habitats. The baseline parameters from the hematological data of the sampled fish will be used to monitor environmental quality.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publishing an article with open access in Veterinary Integrative Sciences leaves the copyright with the author. The article is published under the Creative Commons Attribution License 4.0 (CC-BY 4.0), which allows users to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited.
References
Andreyeva, A., Mukhanov, V., 2012. Method of intravital morphometry of the nucleated erythrocytes of fishes. Hydrobiol. J. 48, 107-112.
Auró de Ocampo, A., Ocampo, L., 1999. Diagnóstico del estrés en peces. Vet. Mex. 30,337–344.
Beeby, A., 2001. What do sentinels stand for?. Environ. Pollut. 112, 285–298.
Carpenter, K.E., 1925. On the biological factor involved in the destruction of river fisheries by pollution due to lead mining. Ann. Appl. Biol. 12, 1–13.
Cossins, A.R., Gibson, J.S., 1997. Volume sensitive transport system and volume homeostasis invertebrate red blood cells. J. Exp. Biol. 200, 343-352.
Da Rocha, C.A.M., Dos Santos, R.A., Bahia, M.D.O., Da Cunha, L.A., Ribeiro, H.F.,Burbano, R.M.R., 2009. The micronucleus assay in fish species as an important tool for xenobiotic exposure risk assessment—a brief review and an example using neotropical fish exposed to methylmercury. Rev. Fish Sci. 17(4), 478-484.
da Silva Souza, T., Fontanetti, C.S., 2006. Micronucleus test and observation of nuclear alterations in erythrocytes of nile tilapia exposed to waters affected by refinery effluent. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 605(1), 87-93.
Dessauer, H.C., 1970. Blood chemistry of reptiles: physiological and evolutionary aspects,Biology of the Reptilia. Academic Press, New York, pp. l-72.
Ergene, S., Cavas, T., Celik, A., Koleli, N., Aymak, C., 2007. Evaluation of river water genotoxicity using the piscine micronucleus test. Environ. Mol. Mutagen. 48,421-429.
Fazio, F., 2019. Fish hematology analysis as an important tool of aquaculture: a review.Aquaculture. 500(1), 237-242.
Fazio, F., Ferrantelli, V., Saoca, C., Giangrosso, G., Piccione, G., 2017. Stability of haematological parameters in stored blood samples of rainbow trout Oncorrhynchus mykiss (Walbaum, 1792). Vet. Med. 62(7), 401-405.
Gordeev, I.I., Mikryakov, D.V., Balabanova, L.V., Mikryakov, V.R., 2017. Composition of leucocytes in peripheral blood of Patagonian toothfish (Dissostichus eleginoides,Smitt, 1898) (Nototheniidae). Pol. Res. 36(1), 1374126.
Guilherme, S., Válega, M., Pereira, M.E., Santos, M.A., Pacheco, M., 2008. Erythrocytic nuclear abnormalities in wild and caged fish (Liza aurata) along an environmental mercury contamination gradient. Ecotoxicol. Environ. Saf. 70, 411–421
Hrubec, T.C., Smith, S.A., Robertson, J.L., 2001. Age related in haematology and chemistry values of hybrid striped bass chrysops Morone saxatilis. Vet. Clin. Pathol. 30, 8–15.
Hutchison, V.H., Szarski, H., 1965. Number of erythrocytes in some amphibians and reptiles.Copeia. 3, 371–375.
IBM, 2017. IBM SPSS Statistics for Windows, Version 25.0. IBM, Armonk, NY.Kendall, R.J., Anderson, T.A., Baker, R.J., Bens, C.M., Carr, J.A., Chiodo, L.A., Cobb III,G.P., Dickerson, R.L., Dixon, K.R., Frame, L.T., Hooper, M.J., Martin, C.F.,McMurry, S.T., Patino, R., Smith, E.E., Theodorakis, C.W., 2001. Ecotoxicology. In: Klaassen, C.D. (Ed.), Casarett and doull’s toxicology. McGraw-Hill, New York, NY.
Keshavarzi, B., Mokhtarzadeh, Z., Moore, F., Mehr, M.R., Lahijanzadeh, A., Rostmi, S.,Kaabi, H., 2015. Heavy metals and polycyclic aromatic hydrocarbons in surface sediments of Karoon River, Khuzestan Province, Iran. Environ. Sci. Pollut. Res. 22,
–19092.
Kumar, R., Banerjee, T.K., 2016. Arsenic induced hematological and biochemical responses in nutritionally important catfish Clarias batrachus (L.). Toxicol. Rep. 3, 148–152.
Li, F., Lin, J.Q., Liang, Y.Y., Gan, H.Y., Zeng, X.Y. Duan, Z.P., Liang, K., Liu, X., Huo, Z.H.,Wu, C.H. 2014. Coastal surface sediment quality assessment in Leizhou Peninsula (South China Sea) based on SEM-AVS analysis. Mar. Pollut. Bull. 84, 424–436.
Nussey, G., Vuren, J.H.J., Preez, du. H.H., 1995. Effect of copper on the differential white blood cell counts of the Mozambique tilapia (Oreochromis mossambicus).Endocrinol. 111, 381-388.
Parrino, V., Cappello, T., Costa, G., Cannavà, C., Sanfilippo, M., Fazio, F., Fasulo, S., 2018. Comparative study of haematology of two teleost fish (Mugil cephalus and Carassius auratus) from different environments and feeding habits. Eur. Zool. J.
(1), 193–199.
Pradit, S., Towatana, P., Nitiratsuwan, T., Jualong, S., Jirajarus, M., Sornpkang, K.,Noppradit, P., Darakai, Y., Weerawong, C., 2020. Occurrence of microplastics on beach sediment at Libong, a pristine island in Andaman Sea, Thailand. Sci. asia. 46,336–434.
Rocha, P.S., Luvizotto, G.L., Kosmehl, T., Bottcher, M., Storch, V., Braunbeck, T., Hollert, H. 2009. Sediment genotoxicity in the Tiete River (Sao Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies. Ecotoxicol. Environ. Saf. 72,
–1848.
Rodriguez-Cea, A., Ayllon, F., Garcia-Vazquez, E., 2003. Micronucleus test in freshwater fish species: an evaluation of its sensitivity for application in field surveys.Ecotoxicol. Environ. Saf. 56, 442–448.
Salkova, E., Gela, D., Pecherkova, P., Flajshans, M. 2022. Examination of white blood cell indicators for three different ploidy level sturgeon species reared in an indoor recirculation aquaculture system for one year. Vet. Med. 67(3), 138–149.
Singkhanan, N., Kettratad, J., Senarat, S., Theerakamol, P., Para, C., Kaneko, G., 2019. Morphological characterization of blood cells in five important estuarine fish species in Thailand during juvenile stages. EnvironmentAsia. 12(2), 79–86.
Strunjak erovic, I., Topic Popovic, N., Coz Rakovac, R., Jadan, M., 2009. Nuclear abnormalities of marine fish erythrocytes. J. Fish. Biol. 74(10), 2239–2249.
Van Ngan, P., Gomes, V., Passos, M., Ussami, K., Campos, D., Rocha, A., Pereira, B.,2007. Biomonitoring of the genotoxic potential (micronucleus and erythrocyte nuclear abnormalities assay) of the Admiralty Bay water surrounding the Brazilian
Antarctic Research Station “Comandante Ferraz”, King George Island. Polar. Biol.30, 209–221.
Wattanayakorn, G., Rungsupa, S., 2012. Petroleum hydrocarbon residues in the marine environment of Koh Sichang-Sriracha, Thailand. Coastal. Mar. Sci. 35, 122-128.
Wilson, J.M., Bunte, R.M., Carty, A.J., 2009. Evaluation of rapid cooling and tricaine methanesulfonate (MS222) as methods of euthanasia in zebrafish (Danio rerio). J. Am. Assoc. Lab. Anim Sci. 48, 785–787.
Witeska, M., 2013. Erythrocytes in teleost fishes: a review. Zool. Ecol. 23(4), 275–281.
Witeska, M., Kondera, E., Szczygielska, K., 2011. The effects of cadmium on common carp erythrocyte morphology. Pol. J. Environ. Stud. 20(3), 783–788.
Witeska, M., Kondera, E., Szymanska, M., Ostrysz, M., 2010. Hematological changes in Common Carp (Cyprinus carpio L.) after short-term lead (Pb) exposure. Pol. J.Environ. Stud. 19(4), 825–831.