The Use of Essential Oil-based Pharmaceutical Products to Control Cattle Ticks

Main Article Content

Apichart Manwicha
Wiwat Pattanawong
Nattha Vigad
Sunee Chansakaow
Pramote Tipduangta
Kridda Chukiatsiri


The purpose of this study was to compare the efficacy of six essential oils obtained from sour orange (Citrus aurantium L.), ginger (Zingiber officinale Roscoe), lemongrass (Cymbopogon citratus (DC.) Stapf.), litsea (Litsea cubeba (Lour.) Pers.), clove (Eugenia caryophyllata Thunb.) and curcumin (Curcuma longa Linn.) against cattle ticks. Two essential oils demonstrated the greatest significant efficiency and were chosen for further testing on the cattle skin. The results showed that the 8% lemongrass essential oil had the most significant inhibiting effect on the oviposition of engorged female cattle ticks, whereas ginger oil, curcumin oil, sour orange, litsea oil and clove oil, showed lesser effect respectively (p<0.05). There was a significant inverse relationship between lemongrass essential oil concentration and the tick’s oviposition index. It was observed that the higher the essential oil concentration (16%), the higher the mortality rate of the larva-stage ticks. Nevertheless, the 4, 8 and 16% of lemongrass essential oil concentrations were also effective against adult ticks, causing a 100% mortality rate. Lemongrass oil can reduce tick number from day 1 after spraying on cattle skin and the tick number was under control for at least 14 days, which is significantly different, comparing to the control group.


Article Details

How to Cite
Apichart Manwicha, Wiwat Pattanawong, Nattha Vigad, Sunee Chansakaow, Pramote Tipduangta, & Chukiatsiri, K. . (2023). The Use of Essential Oil-based Pharmaceutical Products to Control Cattle Ticks: Veterinary Integrative Sciences, 22(1), 55–63. Retrieved from
Research Articles


Chungsamarnyart, N., Jansawan, W., 1996. Acaricidal activity of peel oil of Citrus spp. on Boophilus microplus. Kasetsart J. (Nat. Sci.). 30, 112-117.

Chaparro-Gutiérrez, J.J., Villar, D., Schaeffer, D.J., 2020. Interpretation of the larval immersion test with ivermectin in populations of the cattle tick Rhipicephalus (Boophilus) microplus from Colombian farms. Ticks. Tick. Borne. Dis. 11(2),101323.

Jailanga, C., Chankaew, T., Nitnoisueb, P., 2009. Tobacco and plaques for the removal of cattle ticks, academic registration no. 52(1)-0511-033. Department of livestockdevelopment, Bangkok, pp. 11.

Laohakunjit, N., Kerdchoechuen, O., Klymuk, K., Tripetch, P., 2007. Effectiveness of limonoid extracts from mandarin seeds for controlling beet army worm. Agric. Sci.J. 38(6), 66-69.

Gonçalves, K., Toigo, E., Ascoli, B., Poser, G., Ribeiro, V., 2007. Effects of solvents and surfactant agents on the female and larvae of cattle tick Boophilus microplus.Parasitol. Res. 100, 1267-1270.

Madreseh-Ghahfarokhi, S., Pirali, Y., Dehghani-Samani, A., Dehghani-Samani, A., 2018. The insecticidal and repellent activity of ginger (Zingiber officinale) and eucalyptus (Eucalyptus globulus) essential oils against Culex theileri Theobald, 1903 (Diptera:

Culicidae). Ann Parasitol. 64(4), 351-360.

Madreseh-Ghahfarokhi, S., Dehghani-Samani, A., Pirali, Y., Dehghani-Samani, A.,2019. Zingiber officinalis and Eucalyptus globulus, potent lethal/repellent agents against Rhipicephalus bursa, probable carrier for zoonosis. J. Arthropod. Borne. Dis.

(2), 214-223.

Nogueira, J., Vinturelle, R., Mattos, C., Tietbohl, L.A.C., Santos, M.G., Da Silva Vaz, I.,Mourão, S.C., Rocha, L., Folly, E., 2014. Acaricidal properties of the essential oil from Zanthoxylum caribaeum against Rhipicephalus microplus. J. Med. Entomol.

(5), 971-975.

Pavela, R., 2005. Insecticidal activity of some essential oils against larvae of Spodoptera littoralis. Fitoterapia. 76, 691-696.

Pazinato, R., Volpato, A., Baldissera, M.D., Santos, R.C., Baretta, D., Vaucher, R.A., Giongo, J.L., Boligon, A.A., Stefani, L.M., Da Silva, A., 2016. In vitro effect of seven essential oils on the reproduction of the cattle tick Rhipicephalus microplus. J. Adv.

Res. 7(6), 1029-1034.

Pérez de León, A.A., Mitchell, R.D., Watson, D.W., 2020. Ectoparasites of cattle. Vet. Clin. N. Am. Food. Anim. Pract. 36(1), 173-185.

Prajapati, V., Tripathi, A., Aggarwal, K., Khanuja, S., 2005. Insecticidal, repellent and oviposition-deterrent activity of selected essential oils against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Bioresour. Technol. 96, 1749-1757.

Ribeiro, V., Avancini, C., Gonçalves, K., Toigo, E., Poser, G., 2008. Acaricidal activity of Calea serrata (Asteraceae) on Boophilus microplus and Rhipicephalus sanguineus.Vet. parasitol. 151, 351-354.

Ribeiro, V., Santos, J., Bordignon, S., Apel, M., Henriques, A., Poser, G., 2010. Acaricidal properties of the essential oil from Hesperozygis ringens (Lamiaceae) on the cattle tick Riphicephalus (Boophilus) microplus. Bioresour. technol. 101, 2506-2509.

Ribeiro, V., Santos, J., Martin, J.R., Schripsema, J., Siqueira, I.R., Poser G., Apel, M.,2011. Acaricidal properties of the essential oil and precocene II obtained from Calea serrata (Asteraceae) on the cattle tick Rhipicephalus (Boophilus) microplus (Acari:

Ixodidae). Vet. parasitol. 179, 195-198.

Singh, G.R., Upadhyay, K., 1993. Essential oils: A potent source of natural pesticides. J. Sci.Ind. Res. 52, 676-683.

Steel, R.G.D., Torrie, J.H., 1984. Principle and procedures of statistics, 2nd edition. McGraw Hill, New York, pp. 633.

Torrents, J., Sarli, M., Rossner, M.V., Toffaletti, J.R., Morel, N., Martínez, N.C., Webster, A., Mangold, A.J., Guglielmone, A.A., Nava, S., 2020. Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina. Res. Vet. Sci. 132, 332-337.

Yang, Y.C., Lee, H.S., Clark, J.M., Ahn, Y.J., 2004. Insecticidal activity of plant essential oils against Pediculus humanus capitis (Anoplura: Pediculidae). J. Med. Entomol. 41(4),699-704.