Cryopreservation of goat spermatozoa in the Mekong Delta region of Vietnam: evaluating cryoprotectant effectiveness https://doi.org/10.12982/VIS.2024.011
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Abstract
Conserving and improving the quality of breeds is crucial for enhancing small ruminant production in Vietnam. To support this purpose, cryopreservation was offered as a promising method for semen preservation and maintaining genetic diversity. Moreover, many small-scale farmers in Mekong Delta rely on goats for both income and nutrition, making them an important part of farming activities. This study aimed to develop a sperm cryopreservation protocol and identify the optimal cryoprotectant for goat sperm in the Mekong Delta region of Vietnam. Semen samples were diluted with TCG-EggYolk freezing medium containing cryoprotectant agent (CPA) in a 1:1 ratio. The samples were then placed in 0.5 mL French straws, cooled to 15 °C and 5 °C, and subjected to nitrogen vapor before being immersed in liquid nitrogen at -196 °C. Experiment 1 utilized Glycerol as the CPA in concentrations of 0 %, 5 %, 8 %, and 11 %. Experiment 2 employed dimethyl sulfoxide (DMSO) as the CPA with the same concentrations. Thawed samples were evaluated for sperm quality. Results indicated that in Experiment 1, increasing the Glycerol concentration improved the overall motility, viability, and membrane integrity of sperm. The best outcomes were achieved with 8 % Glycerol, resulting in progressive motility of 57.5 % (P<0.05). In Experiment 2, the extender containing 8 % DMSO demonstrated significantly better performance in terms of overall motility, viability, and membrane integrity compared to 5 % or 11 % DMSO. Progressive motility was 53.5 % (P<0.05). In conclusion, the study determined that using 8 % Glycerol or 8 % DMSO is the optimal choice for freezing goat sperm in the Mekong Delta region of Vietnam.
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References
Aboagla, E.M., Terada, T., 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology. 62(6), 1160-1172.
Alvarez, J.G., Storey, B.T., 1992. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. J. Androl. 13(3), 232-241.
Bagchi, A., Woods, E.J., Critser, J.K., 2008. Cryopreservation and vitrification: recent advances in fertility preservation technologies. Expert Rev. Med. Devices. 5(3), 359‒ 370
Benson, J.D., Woods, E.J., Walters, E.M., Critser, J.K., 2012. The cryobiology of spermatozoa.Theriogenology. 78(8), 1682-1699.
Björndahl, L., Söderlund, I., Kvist, U., 2003. Evaluation of the one step eosin-nigrosin staining technique for human sperm vitality assessment. Hum. Reprod. 18(4), 813‒816.
Bryan Salinas, M., Chuammitri, P., Sringarm, K., Boonyayatra, S., Sathanawongs, A., 2021.Current perspectives on ruminant sperm freezability: Harnessing molecular changes related to semen quality through omics technologies. Vet. Integr. Sci. 19(3), 487-511.
Chemineau, P., Caignie, Y., Guerin, Y., Orgeur, P., Vallet, J.C., 1991. Training manual on artificial insemination in sheep and goats. FAO, Rome, pp. 1014-1197.
Chunrong, L., Wu, G., Hong, Q., Quan, G., 2019. Spermatozoa cryopreservation: state of art and future in small ruminants. Biopreserv. Biobank. 17(2), 171-182.
Farshad, A., Khalili, B., Fazeli, P., 2009. The effect of different concentration of Glycerol and DMSO on viability of Markhoz goat spermatozoa during different freezing temperatures steps. Pak. J. Biol. Sci. 12(3), 239‒245.
Fumuso, F.G., Giuliano, S.M., Chaves, M.G., Neild, D.M., Miragaya, M.H., Gambarotta, M.C., Carretero, M.I., 2018. Seminal plasma affects the survival rate and motility pattern of raw llama spermatozoa. Anim. Reprod. Sci. 192, 99‒106.
Gao, D., Liu, J., Liu, C., Mcgann, L.E., Watson, P.F., Kleinhans, F.W., Mazur, P., Critser, E.S.,Critser, J.K., 1995. Prevention of osmotic injury to human spermatozoa during addition and removal of glycerol. Hum. Reprod. 10(5), 1109‒1122.
Holt, W.V., 2000. Basic aspects of frozen storage of semen. Anim. Reprod. Sci. 62(1-3), 3‒22.
Jeyendran, R.S., Van der Ven, H.H., Perez-Pelaez, M., Crabo, B.G., Zaneveld, L.J.D., 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Infertil. 70,
‒228.
Khuong, T.T.T., Thanh, L.P., Khang, N.T.K., Ngu, N.T., Tuyen, D.N.D., 2022. Cryobank: rapid re-herding solutions for livestock after disease. Can Tho University J. Sci. 58,104‒114.
Küçük, N., Aksoy, M., Uçan, U., Ahmad, E., Naseer, Z., Ceylan, A., Serin, I., 2014. Comparison of two different cryopreservation protocols for freezing goat semen. Cyobiology.68(3), 327‒331.
Kulaksiz, R., Ari, U.C., Daskin, A., Uner, A.G., 2013. The effect of different glycerol concentrations on freezability of semen from Angora, Kilis and Saanen goats. Slovak J. Anim. Sci. 46(2), 39‒44.
Kundu, C.N., Chakraborty, J., Dutta, P., Bhattacharyya, D., Ghosh, A., Majumder, G.C., 2000.Development of a simple sperm cryopreservation model using a chemically defined medium and goat cauda epididymal spermatozoa. Cryobiology. 40(2), 117‒125.
Leboeuf, B., Delgadillo, J.A., Manfredi, E., Piacère, A., Clément, V., Martin, P., Pellicer, M.,Boué, P., Cremoux R.D., 2008. Management of goat reproduction and insemination for genetic improvement in France. Reprod. Domest. Anim. 43(2), 379‒385
Mocé, E., Vicente, J.S., 2003. Effect of freezing–thawing protocols on the performance of semen from three rabbit lines after artificial insemination. Theriogenology. 60(1),115‒123.
Nitira, A., Junpen S., Theerawat, T., Nawapen, P., Jinda, S., Mongkol, T., 2013. Glycerol concentration effects on quality and longevity of post-thaw goat semen. Thai J. Vet.Med. 43(2). 179‒186.
NRC, 2007. National research council: nutrient requirements of small ruminants. National Academy Press, Washington D.C. Pilar, J.R., García-Álvarez, O., Vidal, A., Maroto-Morales, A., Iniesta-Cuerda, M., Ramón, M.,del Olmo, E., Fernández-Santos, R., Garde, J.J., Soler, A.J., 2015. Effects of vitrification on ram spermatozoa using free-egg yolk extenders. Cryobiology. 71(1),85-90.
Purdy, P.H., 2006. A review on goat sperm cryopreservation. Small. Rumin. Res. 63(3), 215‒ 225.
Rini, D.C.P., Zaenab, S., Hadi, S. and Miharja, F.J., 2019. The quality of ettawa crossbreed sperm: the treatment with a combination of cryoprotectant in tris diluents. IOP Conf.Ser. Earth Environ. Sci. 276, 012047.
Salinas, M.B.S., Chuammitri, P., Sringarm, K., Boonyayatra, S., Sathanawongs, A., 2021.Current perspectives on ruminant sperm freezability: harnessing molecular changes related to semen quality through omics technologies. Vet. Integr. Sci. 19(3), 487‒511.
Sánchez, R., Risopatrón, J., Schulz, M., Villegas, J.V., Isachenko, V., Isachenko, E.,2012. Vitrified sperm banks: The new aseptic technique for human spermatozoa allows cryopreservation at −86 °c. Andrologia. 44(6), 433-435.
Taiwo, A.S., James, O.D., Okanlawon, M.O., Adebayo, V.J., Ezekiel, O.A., 2019. Soybean milk extender improves sperm functional and oxidative stress parameters of goat sperm during slow and rapid freezing. Agric. Trop. Subtrop. 52(3-4), 89‒96.
Tamburrino, L., Traini, G., Marcellini, A., Vignozzi, L., Baldi, E., Marchiani, S., 2023.Cryopreservation of human spermatozoa: functional, molecular and clinical aspects. Int. J. Mol. Sci. 24(5), 4656.
Valentina, L., Gianluigi, Z., Alessio, C., Angela, S., Giuseppe, A., Luigi, N., Domenico, R.,Salvatore, C., Gianluca, N., 2020. crocin improves the quality of cryopreserved goat semen in different breeds. Anim. Reprod. 10(6), 1101.
Watson, P., 1995. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev. 7(4), 871-891.
Watson, J.P., Bennett, M.K., Griffin, S.M., Matthewson, K., 2000. The tissue effect of argon plasma coagulation on esophageal and gastric mucosa. Gastrointest. Endosc. 52(3),342‒345.
World Health Organization, 2021. WHO laboratory manual for the examination and processing of human semen, 5th edition. WHO, Geneva.