The effects of sappanwood (Caesalpinia sappan) addition on in vitro methane mitigation, gas production, and ruminal fermentation parameters https://doi.org/10.12982/VIS.2024.074
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Abstract
This research was designed to examine the methane-mitigating properties of sappanwood and its effects on ruminal fermentation parameters using the in vitro gas production method from Menke and Steingass (1988). The sappanwood was dried and pulverised to obtain the sappanwood powder, which was added to 300 mg of forage:concentrate substrate (70:30) at three different levels (2, 4, and 6% DM basis). The in vitro incubation followed a randomised block design and was incubated for 48 hours. The continuous data was then analysed using mixed models of the mixed procedure in SAS on Demand (SAS Institute, Inc. Carry). The results showed that all treatment levels did not affect total gas production, the gas fractions, NH3 levels, microbial proteins, and protozoal counts (P>0.05). The total CH4 and CO2 productions, CH4 and CO2 productions per mg dry matter (DM) and organic matter (OM) degraded, digested dry matter (DDM), digested organic matter (DOM), pH values, total VFAs, acetic, propionic, and butyric acid productions differed significantly (P<0.05). The results emphasizing the 6% addition of sappanwood showed the best methane mitigating properties without hindering ruminal fermentation processes and ruminal microbes.
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References
AOAC, 2005. Official method of analysis of The Association of Official Analytical Chemist,18th edition. AOAC, Maryland.
Ayemele, A.G., Ma, L., Li, X., Yang, P., Xu, J., Yu, Z., Bu, D., 2021. Identification of bioactive phytochemicals from six plants: mechanistic insights into the inhibition of rumen protozoa, ammoniagenesis, and α-glucosidase. Biology. 10(10), 1055.
Chaney, A.L., Marbach, E.P., 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8(2), 130-132.
Chanwitheesuk, A., Teerawutgulrag, A., Rakariyatham, N., 2005. Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food. Chem.92(3), 491-497.
Cherdthong, A., Khonkhaeng, B., Foiklang, S., Wanapat, M., Gunun, N., Gunun, P., Chanjula,P., Polyorach, S., 2019. Effects of supplementation of Piper sarmentosum leaf powder on feed efficiency, rumen ecology and rumen protozoal conncentration in Thai native beef cattle. Animals. 9(4), 130.
Diaz, A., Avendano, M., Escobar, A., 1993. Evaluation of Sapindus Saponaria as a defaunating agent and its effects on different ruminal digestion parameters. Livest. Res. Rural.Dev. 5(2), 1-6.
Filipek, J., Dvorak, R., 2009. Determination of the volatile fatty acid content in the rumen liquid: Comparison of gas chromatography and capillary isotachophoresis. Acta Vet.Brno. 78, 627-633.
Grandón, A.S., Espinosa, B.M., Ríos, D.L., Sánchez, O.M., Sáez, C.K., Hernández, S.V.,Becerra, A.J., 2013. Variation of saponin contents and physiological status in Quillaja saponaria under different environmental conditions. Nat. Prod. Commun. 8(12),
-1700.
Gunun, P., Gunun, N., Cherdthong, A., Wanapat, M., Polyorach, S., Sirilaophaisan,S.,Wachirapakorn, C., Kang, S., 2018. In vitro rumen fermentation and methane production as affected by rambutan peel powder. J. Appl. Anim. Res. 46(1), 626-631.
Hess, H.D., Kreuzer, M., Diaz, T.E., Lascano, C.E., Carulla, J.E., Soliva, C.R., Machmüller,A., 2003. Saponin rich tropical fruits affect fermentation and methanogenesis in faunated and defaunated rumen fluid. Anim. Feed. Sci. and Technol. 109, 79-94.
Jayanegara, A., Wina, E., Takahashi, J., 2014. Meta-analysis on methane mitigating properties of saponin-rich sources in the rumen: influence of addition levels and plant sources.Asian- australas. J. Anim. Sci. 27(10), 1426-1435.
Ku-Vera, J.C., Jiménez-Ocampo, R., Valencia-Salazar, S.S., Montoya-Flores, M.D., MolinaBotero, I.C., Arango, J., Goméz-Bravo, C.A., Aguilar-Pérez, C.F., Solorio-Sánchez, F.J., 2020. Role of secondary plant metabolites on enteric methane mitigation in
ruminants. Front. Vet. Sci. 7, 584.
McDonald, P., Edwards, R.A., Greenhalgh, J.F.D., Morgan, C.A., Sinclair, L.A., Wilkinson,R.G., 2010. Animal nutrition, 7th edition. Prentice Hall, England, pp. 177.
Menke, K.H., Steingass, H., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 7-55.
Nirmal, N.P., Rajput, M.S., Prasad, R.G.S.V., Ahmad, M., 2015. Brazilin from Caesalpinia sappan heartwood and its pharmalogical activities: a review. Asian Pac. J. Trop. Med.8(6), 421-430.
Ørskov, E.R., McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92,499-503.
Oskoueian, E., Abdullah, N., Oskoueian, A., 2013. Effects of flavonoids on rumen fermentation activity, methane production, and microbial population. Biomed. Res. Int. 2013,349129.
Pérez-Barbería, F.J., 2020. The ruminant: Life history and digestive physiology of a symbiotic animal. In: García-Yuste, S. (Ed.), Sustainable and environmentally friendly dairy farms. Springer, Cham, pp. 19-45.
Phesatcha, B., Phesatcha, K., Viennaxay, B., Matra, M., Totakul, P., Wanapat, M., 2022.Cricket meal (Gryllus bimaculatus) as a protein supplement on in vitro fermentation characteristics and methane mitigation. Insects. 13(2), 129.
Phuyal, N., Jha, P.K., Raturi, P.P., Rajbhandary, S., 2020. Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC. Sci. World. J. 2020, 8780704.
Plummer, D.T, 1987. An introduction to practical biochemistry, 3rd edition. McGraw-Hill Book, New Delhi.
Sommai, S., Cherdthong, A., Suntara, C., So, S., Wanapat, M., Polyorach, S., 2021. In vitro fermentation characteristics and methane mitigation responded to flavonoid extract levels from Alternanthera sissoo and dietary ratios. Fermentation. 7(3), 109.
Strobel, H.J., Russell, J.B., 1986. Effect of pH and energy spilling on bacterial protein synthesis by carbohydrate-limited cultures of mixed rumen bacteria. J. Dairy. Sci. 69, 2941-2947.
Vij, T., Anil, P.P., Shams, R., Dash, K.K., Kalsi, R., Pandey, V.K., Harsányi, E., Kovács, B.,Shaikh, A.M., 2023. A comprehensive review on bioactive compounds found in Caesalpinia sappan. Molecules. 28(17), 6247.
Yan, H.X., Sun, L.S., Zhao, G.Q., 2007. Effect of β-carotene on selected indices of in vitro rumen fermentation in goats. J. Anim. Feed. Sci. 16(2), 581–585.