Effect of bacteriophage on histopathology and disease resistance of Whiteleg shrimp (Litopenaeus vannamei) infected by Vibrio parahaemolyticus https://doi.org/10.12982/VIS.2024.036
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Abstract
Vibrio parahaemolyticus is the causative agent of shrimp diseases, the most serious of which is acute hepatopancreatic necrosis disease (AHPND). Bacteriophage is a virus of bacteria that can parasitize and destroy bacteria, so it is considered a potential alternative to antibiotics. The study was carried out to evaluate the treatment ability of bacteriophages on Litopenaeus vannamei after being infected by V. parahaemolyticus B4XOT2.2 isolated from the bottom mud of shrimp ponds infected with AHPND. The study used the histopathological survey method on shrimp at all three ages: postlarvae, adult shrimp at the ages of 30–45 days, and 55–60 days old, which were arranged into three treatments: healthy shrimp, diseased shrimp, and bacteriophage-treated shrimp. The results showed that: the concentration of Vibrio spp. in bacteriophage-treated treatment was decreased in all three groups (postlarvae, shrimp at the ages of 30–45 days, and 55–60 days old) from 3,7x103 CFU/mL to 2,2x102 CFU/mL after two days, from 4,6x104 CFU/mL to 3,3x102 CFU/mL after two days and from 4,6x104 CFU/mL to 5,6x103 CFU/mL after three days, respectively. The pathological signs and histological features of the infected shrimp samples were similar to those typical of acute hepatopancreatic necrosis disease and recovered in shrimp tissue after being treated with bacteriophages such as the hepatopancreas was also darker, the intestines gradually filled, no hematoma around the tubules were found, in the lumen of the tube reduced sloughing cells and blood cells, no melanization was observed.
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References
Aguilar-Rendón, K.G., Lozano-Olvera, R., Yáñez-Rivera, B., Soto-Rodriguez, S.A., 2020.Bacteriological and histopathological analysis of Penaeus vannamei experimentally infected with Vibrio parahaemolyticus-AHPND strains. Dis. Aquat. Organ. 140,167-177.
Baticados, M.C.L., Paclibare, J.O., 1992. The use of chemotherapeutic agents in aquaculture in the Philippines. In: Shariff, M., subasinghe, R.P., Arthur, J.R., (Eds.), Diseases in asian aquaculture I. Fish Health Section. Asian Fisheries Society, Manila,Philippines.
Culot, A., Grosset, N., Gautier, M. 2019. Overcoming the challenges of phage therapy for industrial aquaculture: a review. Aquaculture. 513, 734423.
Harada, L.K., Silva, E.C., Campos, W.F., Del Fiol, F.S., Vila, M., Dąbrowska, K., Krylov,V.N., Balcão, V.M., 2018. Biotechnological applications of bacteriophages: state of the art. Microbiol. Res. 212–213, 38–58.
Holmstrom, K., Graslund, S., Wahlstrom, A., Poungshompoo, S., Bentsson, B.E., Kautsky, N., 2003. Antibiotic use in shrimp farming and implications for environmental impacts and human health. Int. J. Food Sci. Technol. 38(3), 255–266.
Holt, C.C., Bass, D., Stentiford, G.D., Giezen, M.V.D., 2021. Understanding the role of the shrimp gut microbiome in health and disease. J. Invertebr. Pathol. 186, 107387.
Joshi, J., Srisala, J., Sakaew, W., Prachumwat, A., Sritunyalucksana, K., Flegel, T.W.,Thitamadee, S., 2014. Identification of bacterial agent (s) for acute hepatopancreatic necrosis syndrome, a new emerging shrimp disease. Suranaree J. Sci. Technol. 21,315–320.
Jun, J.W., Han, J.E., Giri, S.S., Tang, K.F.J., Zhou, X., Aranguren, L.F., Kim, H.J., Yun,S., Chi, C., Kim, S.G., Park, S.C., 2018. Phage application for the protection from acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei. Indian J.Microbiol. 58(1), 114–117.
Kim, Y.B., Okuda, J., Matsumoto, C., Takahashi, N., Hashimoto, S., Kum Nishibuchi, M.,1999. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J. Clin. Microbiol. 37, 1173–1177.
Kowalska, J.D., Kazimierczak, J., Sowinska, P.M., Wojcik, E.A., Siwicki, A.K., Dastych, J.,2020. Growing trend of fighting infections in aquaculture environment –opportunities and challenges of phage therapy. Antibiotics. 9, 301.
Kropinski, A.M., Mazzocco, A., Waddell, T.E., Lingohr, E., Johnson, R.P., 2009.Enumeration of bacteriophage by double agar overlay plaque assay. Methods Mol.Bio. 501, 69–76.
Kutter, E., Sulakvelidze, A., 2004. Bacteriophages: biology and applications. CRC press,Boca Raton.
Lightner, D.V., Redman, R.M., Pantoja, C.R., Noble, B.L., Tran, L., 2012. Early mortality syndrome affects shrimp in Asia. Glob. Aquac. Advocate. 40.
Tran, L., Nunan, L., Redman, R.M., Mohney, L.L., Pantoja, C.R., Fitzsimmons, K., Lightner,D.V., 2013. Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting Penaeid shrimp. Dis. Aquat. Organ.105(1), 45–55.
Phuong, L.N.N., Anh, L.H., Huan, P.K.N., Loc, H.T., Trang, C.T.H., Mo, T.T.H., Xuan, N.H.,Ngu, N.T., 2022. The effect of different media and temperature conditions for Salmonella bacteriophage preservation. Vet. Integr. Sci. 20(2), 489–496.
Sanders, E.R., 2012. Aseptic laboratory techniques: plating methods. J. Vis. Exp. 63, e3064.
Muthukrishnan, S., Defoirdt, T., Ina-Salwany, M. Y., Yusoff, F. M., Shariff, M., Ismail, S. I.,Natrah, I., 2019. Vibrio parahaemolyticus and Vibrio harveyi causing Acute Hepatopancreatic Necrosis Disease (AHPND) in Penaeus vannamei (Boone, 1931) isolated from Malaysian shrimp ponds. Aquaculture. 511, 734227.
Tan, C.W., Rukayadi, Y., Hasan, H., Abdul-Mutalib, N.-A., Jambari, N.N., Hara, H., Thung,T.Y., Lee, E., Radu, S., 2021. Isolation and characterization of six Vibrio parahaemolyticus lytic bacteriophages from seafood samples. Front. Microbiol. 12,616548.
Thammatinna, K., Egan, M.E., Htoo, H.H., Khanna, K., Sugie, J., Nideffer, J.F., Villa, E.,Tassanacajon, A., Pogliano, J., Nonejuie, P. Chaikeeratisak, V., 2020. A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery. Sci Rep. 10, 2347.
Treiber, F.M., Beranek-Knauer, H., 2021. Antimicrobial residues in food from animal origin—a review of the literature focusing on products collected in stores and markets worldwide. Antibiotics. 10, 534.
Wong, H.C., Wang, T.Y., Yang, C.W., Tang, C.T., Ying, C., Wang, C.H., Chang, W.H., 2019. Characterization of a lytic vibriophage VP06 of vibrio parahaemolyticus. Res.Micro. 170(1), 13-23.
Yang, M., Liang, Y., Huang, S., Zhang, J., Wang, J., Chen, H., Ye, Y., Gao, X., Wu, Q., Tan,Z., 2020. Isolation and characterization of the novel phages vB_VpS_BA3 and vB_VpS_CA8 for lysing Vibrio parahaemolyticus. Front. Microbiol. 11, 259.