Effect of By-Product from C-Phycocyanin Extraction as a Prebiotic Properties and Probiotic Microbial Population https://doi.org/10.12982/VIS.2025.003
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Abstract
This study was designed to investigate the efficacy of alternative prebiotic supplements derived from phycocyanin extraction by-product in enhancing the growth of probiotic. These alternative prebiotic supplements included Spirulina platensis, the by-product of phycocyanin extraction, and inulin. The efficacy of alternative prebiotics was assessed using beneficial bacteria (Lactobacillus johnsonii ck-3 and ck-8), pathogenic bacteria (Staphylococcus aureus and Escherichia coli), and yeast (Saccharomyces cerevisiae). The alternative prebiotics source was used to analyse the chemical composition, prebiotic activity, and bacteria growth. It was demonstrated that the phycocyanin extraction by-product included a high concentration of crude protein (67.74% DM), carbohydrates (31.29% DM). The phycocyanin extraction by-product has a high content of glucose, fructose, and sucrose (63.55, 68.00, and 0.24 µg/ml, respectively). The phycocyanin extraction by-product has presented hydrolysis ability (in vitro) at 2.51% while S. platensis showed at 1.81 %. The composition of the phycocyanin extraction by-product showed similar activation of probiotic bacteria to inulin. Especially, Lactobacillus strain showed the best growth was achieved with the phycocyanin extraction by-product with decrease the final pH and high microbial colony number at 48 hours in 7.5–9.5 log10 colony-forming unit per milliliter (CFU/ml) when compared with the glucose group. The study results revealed that the phycocyanin extraction by-product with the prebiotic potential are effective at increasing numbers of Lactobacillus strain. Consequently, the study reveals that phycocyanin extraction by-product are effective as alternative prebiotic supplement, significantly enhancing beneficial probiotic bacteria. These findings suggest the potential use of phycocyanin extraction by-products in creating new prebiotic formulations for gut microbiota modulation through dietary
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