Evaluation of quality and sex ratio of sperm in post-thaw sexed Brahman semen using magnetic-activated cell sorting conjugated with Y-scFv antibodies (M-Zlex) https://doi.org/10.12982/VIS.2024.073
Article Sidebar
Main Article Content
Abstract
Brahman cattle (Bos indicus) are the breed suitable for beef production in tropical climate rearing conditions, where the use of sexed semen helps to pass on the good genetics of the superior sire and expand the herd rapidly and efficiently. This research evaluated the motility, kinematic variables, and sex ratio of sperm in post-thaw sexed Brahman semen using magnetic-activated cell sorting conjugated with Y-scFv antibodies (M-Zlex) by computer-assisted sperm analysis (CASA) and imaging flow cytometry. Fresh semen samples from a total of 26 ejaculates were collected from eight Brahman bulls and divided into two parts to produce conventional (CONV) semen and M-Zlex semen. Then, CASA was used to evaluate sperm motility and kinematic variables, and imaging flow cytometry was used to assess the X-/Y-chromosome bearing sperm ratio. The results found that sperm motility and all kinematic variables of M-Zlex semen did not differ from those of CONV semen. A significant difference was observed between M-Zlex and CONV in the proportion of X-sperm (p < 0.05) in eight bulls. 76.40%–80.90% X-sperm was present in M-Zlex, while the range of CONV was between 48.07% and 51.90%. Therefore, M-Zlex sexing method is another alternative for producing sexed Brahman bull sperm, which has the same sperm quality as CONV but increases the number of X-chromosome-bearing sperm that tend to produce female calves.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publishing an article with open access in Veterinary Integrative Sciences leaves the copyright with the author. The article is published under the Creative Commons Attribution License 4.0 (CC-BY 4.0), which allows users to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited.
References
Akyol, N., Varisli, O., Kızıl, S., 2018. Effects of long-term storage on some spermatological parameters in cryopreserved bull semen. CryoLetters. 36(6), 354-358.
Bahmid, N.A., Karja, N.W.K., Arifiantini, R.I., 2023. The quality of frozen friesian holstein semen after long-term storage. Trop. Anim. Sci. J. 46(1), 13–19.
Bessa, A.F., de O., Duarte, I.N.H., Rola, L.D., Bernardes, P.A., Gonzaga Neto, S., Lôbo, R.B., Munari, D.P., Buzanskas, M.E., 2021. Genetic evaluation for reproductive and productive traits in Brahman cattle. Theriogenology. 173, 261–268.
Chaokaur, A., Nishida, T., Phaowphaisal, I., Sommart, K., 2015. Effects of feeding level on methane emissions and energy utilization of Brahman cattle in the tropics. Agric.Ecosyst. Environ. 199, 225–230.
Chowdhury, M.M.R., Lianguang, X., Kong, R., Park, B.Y., Mesalam, A., Joo, M.D., Afrin,F., Jin, J.I., Lim, H.T., Kong, I.K., 2019. In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes. Anim.Reprod. Sci. 205, 156–164.
Crites, B.R., Vishwanath, R., Arnett, A.M., Bridges, P.J., Burris, W.R., McLeod, K.R.,Anderson, L.H., 2018. Conception risk of beef cattle after fixed-time artificial insemination using either SexedUltraTM 4M sex-sorted semen or conventional semen. Theriogenology. 118, 126–129.
DeJarnette, J.M., Nebel, R.L., Marshall, C.E., 2009. Evaluating the success of sex-sorted semen in US dairy herds from on farm records. Theriogenology. 71(1), 49–58.
Habermann, F.A., Winter, A., Olsaker, I., Reichert, P., Fries, R., 2005. Validation of sperm sexing in the cattle (Bos taurus) by dual colour fluorescence in situ hybridization. Anim. Breed. Genet. 122, 22–27.
Jo, S.Y., Hwangbo, Y., Lee, S.H., Cheong, H.T., Kim, D.K., Park, C.K., 2021. Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics. J. anim. reprod. biotechnol. 36(4), 239–246.
Kamprasert, N., Duijvesteijn, N., Van Der Werf, J.H.J., 2019. Estimation of genetic parameters for BW and body measurements in Brahman cattle. Anim. 13(8), 1576–1582.
Martínez-Pastor, F., Mata-Campuzano, M., Álvarez-Rodríguez, M., Álvarez, M., Anel, L.,de Paz, P., 2010. Probes and techniques for sperm evaluation by flow cytometry.Reprod. Domest. Anim. 45(SUPPL. 2), 67–78.
Mostek, A., Janta, A., Ciereszko, A., 2020. Proteomic comparison of non-sexed and sexed (X-bearing) cryopreserved bull semen. Anim. Reprod. Sci. 221, 106552.
Oosthuizen, N., Fontes, P.L.P., Oliveira Filho, R.V., Dahlen, C.R., Grieger, D.M., Hall, J.B., Lake, S.L., Looney, C.R., Mercadante, V.R.G., Neville, B.W., Perry, G.A.,Powell, J.G., Prezotto, L.D., Seidel, G.E., Walker, R.S., Cardoso, R.C., Pohler, K.G.,Lamb, G. C., 2021. Pre-synchronization of ovulation timing and delayed fixedtime artificial insemination increases pregnancy rates when sex-sorted semen is used for insemination of heifers. Anim. Reprod. Sci. 226, 106699.
Paitoon, P., Sartsook, A., Thongkham, M., Sathanawongs, A., Lumsangkul, C., Pattanawong,W., Hongsibsong, S., Sringarm, K., 2024. Sperm quality variables of sex-sorted bull semen produced by magnetic-activated cell sorting coupled with recombinant
antibodies targeting Y-chromosome-bearing sperm. Theriogenology. 219, 11-21.
Pfeifer, L.F.M., Júnior, J.S.O., Potiens, J.R., 2019. Effect of sperm kinematics and size of follicle at ovulation on pregnancy rate after timed AI of beef cows. Anim. Reprod.Sci. 201, 55–62.
Purdy, P.H., Graham, J.K., Azevedo, H.C., 2022. Evaluation of boar and bull sperm capacitation and the acrosome reaction using flow cytometry. Anim. Reprod. Sci. 246.
Qi, X., Zhang, Y., Xue, L., Chen, W., Zhang, G., Hou, L., 2024. An objective quality indicator for evaluating filtering of microscopic video in CASA system. Biomed.Signal. Process. Control. 88, 105604.
Quan, G.B., Ma, Y., Li, J., Wu, G.Q., Li, D.J., Ni, Y.N., Lv, C.R., Zhu, L., Hong, Q.H., 2015. Effects of Hoechst33342 staining on the viability and flow cytometric sex-sorting of frozen-thawed ram sperm. Cryobiology. 70(1), 23–31.
Quelhas, J., Santiago, J., Matos, B., Rocha, A., Lopes, G., Fardilha, M., 2021. Bovine semen sexing: Sperm membrane proteomics as candidates for immunological selection of X- and Y-chromosome-bearing sperm. Vet. Med. Sci. 7(5), 1633–1641.
Sang, L., Yang, W.C., Han, L., Liang, A.X., Hua, G.H., Xiong, J.J., Huo, L.J., Yang, L.G.,2011. An immunological method to screen sex-specific proteins of bovine sperm. J.Dairy. Sci. 94(4), 2060–2070.
Santarosa, B.P., Guerra, S.T., Ferreira, D.O.L., Polizel, D.M., Padilha, L.M., Pereira, P.F. V.,Dal Más, F.E., Pimenta-Oliveira, A., Oliveira-Filho, J.P., Gonçalves, R.C., 2023.Blood gas parameters of Brahman cattle. Animal Open Space. 2, 100047.
Soleymani, B., Mansouri, K., Rastegari-Pouyani, M., Parvaneh, S., Khademi, F., Sharifi Tabar, M., Mostafaie, A., 2020. Production of monoclonal antibody against recombinant bovine sex-determining region Y (SRY) and their preferential binding to Y chromosome-bearing sperm. Reprod. Domest. Anim. 56, 270–277.
Sringarm, K., Thongkham, M., Mekchay, S., Lumsangkul, C., Thaworn, W., Pattanawong, W., Rangabpit, E., Rachtanapun, P., Jantanasakulwong, K., Sathanawongs, A., Hongsibsong, S., 2022. High-efficiency bovine sperm sexing used magneticactivated cell sorting by coupling scFv antibodies specific to Y-chromosome-bearing sperm on magnetic microbeads. Biol. 11(5), 715.
Thaworn, W., Hongsibsong, S., Thongkham, M., Mekchay, S., Pattanawong, W., Sringarm,K., 2022. Production of single-chain fragment variable (scFv) antibodies specific to plasma membrane epitopes on bull Y-bearing sperm. Anim. Biotechnol. 33(3), 508-518.
Thomas, J.M., Locke, J.W.C., Bonacker, R.C., Knickmeyer, E.R., Wilson, D.J., Vishwanath,R., Arnett, A.M., Smith, M.F., Patterson, D.J., 2019. Evaluation of SexedULTRA 4MTM sex-sorted semen in timed artificial insemination programs for mature beef
cows. Theriogenology. 123, 100–107.
Thomas, J.M., Locke, J.W.C., Vishwanath, R., Hall, J.B., Ellersieck, M.R., Smith, M.F.,Patterson, D.J., 2017. Effective use of SexedULTRATM sex-sorted semen for timed artificial insemination of beef heifers. Theriogenology. 98, 88–93.
Thongkham, M., Thaworn, W., Pattanawong, W., Teepatimakorn, S., Mekchay, S., Sringarm,K., 2021. Spermatological parameters of immunologically sexed bull semen assessed by imaging flow cytometry, and dairy farm trial. Reprod. Biol. 21(2),
Uhm, S.J., Heo, Y.T., Yu, D.M., Kim, D.K., Gupta, M.K., 2023. Pre-implantation development of cattle embryos produced from fresh bull semen enriched for Xchromosome-bearing spermatozoa using a monoclonal antibody. Vet. Res. Commun.
, 2101-2109.
Waritthitham, A., Lambertz, C., Langholz, H.J., Wicke, M., Gauly, M., 2010. Assessment of beef production from Brahman × Thai native and Charolais × Thai native crossbred bulls slaughtered at different weights. I: Growth performance and carcass
quality. Meat. Sci. 85(1), 191–195