Toxoplasma gondii contamination in fresh vegetables destined for human and livestock consumption in Thua Thien Hue, Vietnam https://doi.org/10.12982/VIS.2024.057
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Abstract
Toxoplasmosis caused by Toxoplasma gondii is an important zoonosis that shows severe symptoms in immunocompromised patients. High seroprevalences of toxoplasmosis were found in cats, humans, and pigs in traditional farms in Thua Thien Hue province. The main sources of infections remain unknown, thus making toxoplasmosis neglected and uncontrollable in this region. This study aimed to determine T. gondii contamination in vegetables used for human and livestock consumption and its spatial distribution in Thua Thien Hue rural areas. A cross-sectional study was conducted to investigate T. gondii contamination in vegetables grown in households in three different geographic regions. The pathogen DNA was detected from vegetable samples using a primer pair that is highly specific for the 529-bp repetitive element found in T. gondii genome. In this study, 55 out of 221 (24.9%) vegetable samples were positive for T. gondii using PCR. Factors, including location, presence of fence in the garden, owning cats, and treatment of feed for cats, were not significantly associated with T. gondii contamination in vegetables according to the logistic regression. However, vegetables collected in households that have no fence for vegetable gardens, own cats, and feed cats raw or undercooked foods tend to be more heavily contaminated than others. Furthermore, a wide distribution of infected vegetables was observed in all investigated districts, showing a high infection pressure in these residential areas. This is the first report of vegetables contaminated with T. gondii in Vietnam, which reveals an overlooked health risk for humans and animals.
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References
Adeela, A., Azhar, M., Muhammad, F.Q., Kamran, A., Aftab, A.A., 2013. Detection of Toxoplasma gondii in environmental matrices (water, soil, fruits, and vegetables).Afr. J. Microbiol. Res. 7(16), 1505–1511.
Al-Megrm, W.A.I., 2010. Prevalence intestinal parasites in leafy vegetables in Riyadh, Saudi Arabia. Int. J. Trop. Med. 5(2), 20–23.
Bawm, S., Phyu, A.Z., Chel, H.M., Htun, L.L., Nakao, R., Katakura, K., 2020. Seroprevalence of Toxoplasma gondii in household cats in Myanmar and molecular identification of parasites using feline faecal oocysts. Food. Waterborne. Parasitol. 20, e00094.
Bourdin, C., Busse, A., Kouamou, E., Touafek, F., Bodaghi, B., Le Hoang, P., Mazier, D.,Paris, L., Fekkar, A., 2014. PCR-based detection of Toxoplasma gondii DNA in blood and ocular samples for diagnosis of ocular toxoplasmosis. J. Clin. Microbiol.52(11), 3987–3991.
de Souza, C.Z., R afael, K., Sanders, A.P., Tiyo, B.T., Marchioro, A.A., Colli, C.M., Gomes,M.L., Falavigna-Guilherme, A.L., 2016. An alternative method to recover Toxoplasma gondii from greenery and fruits. Int. J. Environ. Health Res. 26(5–6),600–605.
Dinh, T.B.L., Huynh, V.C., 2009. Detection of antibodies to Toxoplasma gondii in humans and livestock in Thua Thien Hue province. Hue Univ. J. Sci. 55, 149155.
Djurković-Djaković, O., Dupouy-Camet, J., Van der Giessen, J., Dubey, J.P., 2019. Toxoplasmosis: overview from a One Health perspective. Food. Waterborne.Parasitol, 15, e00054.
Dubey, J.P., Lindsay, D.S., Speer, C.A., 1998. Structures of Toxoplasma gondii Tachyzoites, Bradyzoites, and sporozoites and biology and development of tissue cysts. Clin.Microbiol. Rev. 11(2), 267.
Escotte-Binet, S., Da Silva, A.M., Cancès, B., Aubert, D., Dubey, J., La Carbona, S., Villena, I., Poulle, M.L., 2019. A rapid and sensitive method to detect Toxoplasma gondii oocysts in soil samples. Vet. Parasitol. 274, 108904.
Homan, W.L., Vercammen, M., De Braekeleer, J., Verschueren, H., 2000. Identification ofa 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int. J. Parasitol. 30(1), 69–75.
Hosono, H., Ito, S., Kono, H., Xuan, X., 2009. Seroprevalence of toxoplasma gondii in cats and pigs from Thua Thien Hue Province in Vietnam. J. Vet. Epidemiol. 13(2), 100–106.
Lalonde, L.F., Gajadhar, A.A., 2016. Detection of Cyclospora cayetanensis, Cryptosporidium spp.,and Toxoplasma gondii on imported leafy green vegetables in Canadian survey.Food. Waterborne. Parasitol. 2, 8–14.
Lass, A., Pietkiewicz, H., Szostakowska, B., Myjak, P., 2012. The first detection of Toxoplasma gondii DNA in environmental fruits and vegetables samples. Eur. J.Clin. Microbiol. Infect. Dis. 31(6), 1101–1108.
Marchioro, A.A., Tiyo, B.T., Colli, C.M., De Souza, C.Z., Garcia, J.L., Gomes, M.L.,Falavigna-Guilherme, A.L., 2016. First detection of toxoplasma gondii dna in the fresh leafs of vegetables in South America. Vector. Borne. Zoonotic. Dis. 16(9), 624–626.
Marín-García, P.J., Planas, N., Llobat, L., 2022. Toxoplasma gondii in foods: prevalence,control, and safety. Foods. 11(16), 2542.
Miller, M.A., 2008. Tissue cyst-forming coccidia of marine mammals, In: Fowler, M.E.,Miller, R.E. (Eds.) Zoo and wild animal medicine, (6th edition). W.B. Saunders,Saint Louis, pp. 319-340.
Montoya, J.G., Liesenfeld, O., 2004. Toxoplasmosis. Lancet. 363(9425), 1965–1976.
Nguyen T.H.D., Ton N.V.A., 2014. Study of Toxoplasma gondii infection in epileptic children. J. Med. Pharm. Hue. Univ. Med. Pharm. 4(3), 63.
Pleyer, U., Groß, U., Schlüter, D., Wilking, H., Seeber, F., 2019. Toxoplasmosis in Germany:epidemiology, diagnosis, risk factors, and treatment. Dtsch. Arztebl. Int. 116(25), 435.
Robert-Gangneux, F., Dardé, M.L., 2012. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin. Microbiol. Rev. 25(2), 264.
Shapiro, K., Bahia-Oliveira, L., Dixon, B., Dumètre, A., de Wit, L.A., VanWormer, E.,Villena, I., 2019. Environmental transmission of Toxoplasma gondii: Oocysts in water, soil, and food. Food. Waterborne. Parasitol. 15, e00049.
Slany, M., Dziedzinska, R., Babak, V., Kralik, P., Moravkova, M., Slana, I., 2019.Toxoplasma gondii in vegetables from fields and farm storage facilities in the Czech Republic. FEMS Microbiol. Lett. 366(14).
Sullivan, W.J., Jeffers, V., 2012. Mechanisms of Toxoplasma gondii persistence and latency.FEMS Microbiol. Rev. 36(3), 717–733.
Templeton, T.J., 2007. The secretory protein repertoire and expanded gene families of toxoplasma gondii and other apicomplexa, In: Weiss, L.M., Kim, K. (Eds.), Toxoplasma gondii: the model apicomplexan. perspectives and methods. Academic Press, London, pp. 445-463.
Udonsom, R., Buddhirongawatr, R., Nishikawa, Y.M., Fereig, R., Jirapattharasate, C., 2021. Toxoplasma gondii prevalence and risk factors in owned domestic cats from Nakhon Pathom Province, Thailand. Vet. Integr. Sci. 19(3), 557–566.
VanWormer, E., Fritz, H., Shapiro, K., Mazet, J.A.K., Conrad, P.A., 2013. Molecules to modeling: Toxoplasma gondii oocysts at the human–animal–environment interface. Comp. Immunol. Microbiol. Infect. Dis. 36(3), 217.
Webster, J.P., 2010. Toxoplasmosis of animals and humans. In: Dubey, J.P. (Ed.), Parasites &vector. 3, 112.