Open-field and tonic immobility responses in broiler chickens administered lycopene https://doi.org/10.12982/VIS.2025.023
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Abstract
The study aimed to evaluate behavioural responses in broiler chickens administered with lycopene and subjected to open field (OF) and tonic immobility (TI) tests. A total of 100 broiler chicks, divided into five groups of 20 chickens each, were used in experiments I and II. Experiment I had control A and lycopene groups, and Experiment II involved control B, habituation (HB), and habituation+lycopene (HB+Lyco) groups. The olive oil or mixed lycopene was administered by oral gavage once daily, commencing at 07:00 h (GMT+1), to each bird for 28 days for Experiment I and 21 days for Experiment II. In Experiment I, the OF test was conducted on days 14 and 28, while in Experiment II, 4-day habituation training was carried out in the HB and HB+Lyco groups from days 17 to 20, followed by the TI test in each of the three groups on day 21. The results of the OF test indicated a reduced (P ˂ 0.05) number of lines crossed, vocalisation, number of jumps, and defecation in the lycopene group compared to control A. An increased (P < 0.05) TI duration was recorded in control B compared to the HB group. Lycopene administration for 21 days increased (P < 0.05) the TI duration value in the HB+Lyco group compared to the HB group. In conclusion, lycopene administration increased fearfulness in broiler chickens, as evidenced by high fear responses during OF and TI tests. This may be due to the decreased ability to habituate to fear-eliciting stimuli in broiler chickens administered lycopene.
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References
Arain, M.A., Mei, Z., Hassan, F.U., Saeed, M., Alagawan M., Shar, A.H., Rajput, I.R., 2018. Lycopene: a natural antioxidant for prevention of heat-induced oxidative stress in poultry. Worlds Poult. Sci. J. 74(1), 89-100.
Ayo, J.O., Obidi, J.A., Rekwot, P.I., Onyeanusi, B.I., 2022. Modulatory effects of lycopene and vitamin E on cloacal temperature, thyroid hormonal and reproductive performance responses in laying hens during the hot-dry season. J. Therm. Biol. 104, 103105.
Bala, R., Khanna, D., Mehan, S., Kalra, S., 2015. Experimental evidence for the potential of lycopene in the management of scopolamine induced amnesia. RSC Advances. 5, 72881-72892.
Balazova, L., Baranyiova, E., 2010. Broiler response to open field test in early ontogeny. Acta Vet. Brno. 79(1), 19-26.
Benoff, F.H., Siegel, P.B., 1976. Genetic analyses of tonic immobility in young Japanese quail (Cortunix cortunix japonica). Anim. Learn. Behav. 67, 226-231.
Chen, D., Huang, C., Chen, Z., 2019. A review for the pharmacological effect of lycopene in central nervous system disorders. Biomed. Pharmacother. 111, 791-801.
Dawkins, M., 2015. Animal welfare and the paradox of animal consciousness. Adv. Study Behav. 47, 5-38
Domjan, M., 2010. The principles of learning and behavior, 6th Edition. Thomson/Wadsworth, Belmont, pp. 284-293.
El Morsy, E.M., Ahmed, M.A.E., 2020. Protective effects of lycopene on hippocampal neurotoxicity and memory impairment induced by bisphenol A in rats. Hum. Exp. Toxicol. 39(8), 1066-1078.
Forkman, B., Boissy, A., Meunier-Salaun, M.C., Canali, E., Jones, R.B., 2007. A critical review of fear tests used on cattle, pigs, sheep, poultry and horses. Physiol. Behav. 92, 340-374.
Grissom, N., Bhatnagar, S., 2009. Habituation to repeated stress: get used to it. Neurobiol. Learn. Mem. 92(2), 215-224.
Harvey, S., Hall, T.R., 1990. Hormones and stress in birds: activation of the hypothalamo-pituitary-adrenal axis. Prog. Clin. Biol. Res. 342, 453-460.
Heiblum, R., Aizenstein, O., Gvaryahu, G., Voet, H., Robinzon, B., Snapir, N., 1998. Tonic immobility and open field responses in domestic fowl chicks during the first week of life. Appl. Anim. Behav. Sci. 60(4), 347-357.
Herman, J.P., 2013. Neural control of chronic stress adaptation. Front. Behav. Neurosci. 7, 61.
Hernandez, E., Llonch, P., Turner, P.V., 2022. Applied animal ethics in industrial food animal production: exploring the role of the veterinarian. Animals. 12(6), 678.
Jones, R.B., 1986. The tonic immobility reaction of the domestic fowl: a review. Worlds Poult. Sci. J. 42, 82-96.
Jones, R.B., Carmichael, N.L., 1997. Open-field behavior in domestic chicks tested individually or in pairs: differential effects of painted lines delineating subdivisions of the floor. Behav. Res. Meth. Instrum. Comput. 29(3), 396-400.
Jones, R.B., Merry, B.J., 1988. Individual or paired exposure of domestic chicks to an open field: some behavioural and adrenocortical consequences. Behav. Process. 16(1-2), 75-86.
Jones, R.B., 1977. Repeated exposure of the domestic chick to a novel environment: effects on behavioural responses. Behav. Process. 2(2), 163-173.
Jones, R.B., 1989. Avian open-field research and related effects of environmental novelty: an annotated bibliography, 1960–1988. Psychol. Rec. 39(3), 397-420.
Joshi, B., Kar, S.K., Yadav, P.K., Yadav, S., Shrestha, L., Bera, T.K., 2020. Therapeutic and medicinal uses of lycopene: a systematic review. Int. J. Med. Sci. 8, 1195.
Katajamaa, R., Jensen, P., 2021. Tameness correlates with domestication related traits in a Red Junglefowl intercross. Genes. Brain. Behav. 20(3), e12704.
Kostal, L., Skalná, Z., Pichova, K., 2020. Use of cognitive bias as a welfare tool in poultry. J. Anim. Sci. 98(Suppl 1), S63-S79.
Kremer, L., Holkenborg, S.K., Reimert, I., Bolhuis, J.E., Webb, L.E., 2020. The nuts and bolts of animal emotion. Neurosci. Biobehav. Rev. 113, 273-286.
Lagadic, H., Faure, J.M., Mills, A.D., Williams, J.B., 1990. Effects of blood sampling on plasma concentrations of corticosterone and glucose in laying hens caged in groups. Br. Poult. Sci. 31, 823-829.
Lourenço da Silva, M.I., Almeida Paz, I.C.D.L., Chaves, G.H.C., Almeida, I.C.D.L., Ouros, C.C.D., Souza, S.R.L., Milbradt, E.L., Caldara, F.R., Satin, A.J.G., Costa, G.A.D., Glavina, A.S.G., 2021. Behaviour and animal welfare indicators of broiler chickens housed in an enriched environment. Plos one, 16(9), e0256963.
Mezbani, A., Kavan, B.P., Kiani, A., Masouri, B., 2019. Effect of dietary lycopene supplementation on growth performance, blood parameters and antioxidant enzymes status in broiler chickens. Available online: http://www.lrrd.org/lrrd31/1/bahma31 012.html.
Nash, R.F., Gallup, G.G., 1976. Habituation and tonic immobility in domestic chickens. J. Comp. Physiol. Psychol, 90(9), 870-876.
Nielsen, B.L., 2020. Asking Animals: An Introduction to Animal Behaviour Testing. CABI, UK, pp. 186.
Ogundeji, T., Ayo, J.O., Aluwong, T., Mohammed, A., 2023. Physiological responses in broiler chickens administered lycopene during the hot-dry season. Folia Vet. 67(4), 10-18.
Ogundeji, T., Ayo, J.O., Aluwong, T., Mohammed, A., 2013. Behavioural and haematological studies on effects of lycopene in Wistar rats subjected to psychological stress. J. Neurosci. Behav. Health. 5(2), 30-35.
Patel, S., Johnson, K., Adank, D., Rosas-Vidal, L.E., 2022. Longitudinal monitoring of prefrontal cortical ensemble dynamics reveals new insights into stress habituation. Neurobiol. Stress, 20, 100481.
Patel, S., Roelke, C.T., Rademacher, D.J., Hillard, C.J., 2005. Inhibition of restraint stress‐induced neural and behavioural activation by endogenous cannabinoid signalling. Eur. J. Neurosci. 21(4), 1057-1069.
Rankin, C.H., 2009. Introduction to special issue of neurobiology of learning and memory on habituation. Neurobiol. Learn. and Mem. 92, 125-126.
Rankin, C.H., Abrams, T., Barry, R.J., Bhatnagar, S., Clayton, D.F., Colombo, J., Coppola, G., Geyer, M.A., Glanzman, D.L., Marsland, S., McSweeney, F.K., Wilson, D.A., Wu, C.F., Thompson, R.F., 2009. Habituation revisited: an updated and revised description of the behavioural characteristics of habituation. Neurobiol. Learn. Mem. 92(2), 135-138.
Sahin, K., Orhan, C., Tuzcu, M., Sahin, N., Hayirli, A., Bilgili, S., Kucuk, O., 2016. Lycopene activates antioxidant enzymes and nuclear transcription factor systems in heat-stressed broilers. Poult. Sci. 95, 1088-1095.
Sinkalu, V.O., Ayo, J.O., Adelaiye, A.B., Hambolu, J.O., 2016. Melatonin modulates tonic immobility and vigilance behavioural responses of broiler chickens to lighting regimens during the hot-dry season. Physiol. Behav. 52, 717-723.
Stanford, S.C., 2007. The open field test: reinventing the wheel. J. Psychopharmacol. 21(2), 134-136.
Walsh, R.N., Cummins, R.A., 1976. The open-field test: a critical review. Psychol. Bull. 83(3), 482-504.
Wang, S., Wu, H., Zhu, Y., Cui, H., Yang, J., Lu, M., Cheng, H., Gu, L., Xu, T., Xu, L., 2022. Effect of lycopene on the growth performance, antioxidant enzyme activity, and expression of Gene in the Keap1-Nrf2 signaling pathway of arbor acres broilers. Front. Vet. Sci. 9, 833346.
Wang, Z., Fan, J., Wang, J., Li, Y., Xiao, L., Duan, D., Wang, Q., 2016. Protective effect of lycopene on high-fat diet-induced cognitive impairment in rats. Neurosci. Lett. 627, 185-191
Wilcox, C.H., Sandilands, V., Mayasari, N., Asmara, I.Y., Anang, A., 2023. A literature review of broiler chicken welfare, husbandry, and assessment. Worlds Poult. Sci. J. 80(1), 3-32.
Zhao, B., Ren, B., Guo, R., Zhang, W., Ma, S., Yao, Y., Yuan, T., Liu, Z., Liu, X., 2017. Supplementation of lycopene attenuates oxidative stress induced neuroinflammation and cognitive impairment via Nrf2/NF-κB transcriptional pathway. Food. Chem. Toxicol. 109(1), 505-516.
Zulkifli, I., Che Norma, M.T., Chong, C.H., Loh, T.C., 2000. Heterophil/lymphocyte and tonic immobility reactions to pre-slaughter handling in broiler chickens treated with ascorbic acid. Poult. Sci. 79, 402-406.