Antibacterial efficacy of essential oils from Eugenia javanica Lam. and Eugenia paniala Roxb. leaves against Staphylococcus chromogenes and Streptococcus uberis for dairy mastitis https://doi.org/10.12982/VIS.2026.007

Main Article Content

Banthita Saengsitthisak
Wasana Chaisri
Sukolrat Boonyayatra
Surachai Pikulkaew
Noppason Pangprasit
Nuttakorn Baisaeng

Abstract

The aim of this research was to explore the antibacterial properties of essential oils derived from the leaves of Eugenia javanica Lam. (E. javanica Lam.) and Eugenia paniala Roxb. (E. paniala Roxb.) against prevalent mastitis-causing bacteria such as Staphylococcus chromogenes (S. chromogenes) and Streptococcus uberis (S. uberis). These oils were extracted from the leaves of E. javanica Lam. and E. paniala Roxb. using hydrodistillation with a Clevenger-type apparatus. Subsequently, the chemical compositions of the crude oils were determined using gas chromatography-mass spectrometry. The yield of the essential oil was found to be 0.05 ± 0.01% (w/w) and 0.12 ± 0.01% (w/w) on a fresh weight basis. The primary compounds identified consisted of γ-Terpinene (30.78%), p-Cymene (12.28%), (E)-Caryophyllene (8.84%), and β-Pinene (8.18%) for E. javanica Lam., while trans-β-ocimene (57.29%), trans-caryophyllene (7.42%), linalool (4.60%), and caryophyllene oxide (3.85%) were the predominant constituents for E. paniala Roxb. The antibacterial activity of each essential oil was assessed through minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) using the broth microdilution method. The MIC and MBC values of the essential oils from E. javanica Lam. and E. paniala Roxb. leaves against each pathogen were compared using the Mann-Whitney U test for pairwise analysis. The results revealed that the essential oil from E. paniala Roxb. leaves exhibited superior inhibitory and bactericidal effects against S. chromogenes with lower MIC and MBC values (P < 0.05). In summary, essential oils extracted from E. javanica Lam. and E. paniala Roxb. leaves demonstrated antibacterial activity against major mastitis pathogens, including S. chromogenes and S. uberis.

Article Details

How to Cite
Saengsitthisak, B. ., Chaisri, W. ., Boonyayatra, S. ., Pikulkaew, S. ., Pangprasit, N. ., & Baisaeng, N. (2025). Antibacterial efficacy of essential oils from Eugenia javanica Lam. and Eugenia paniala Roxb. leaves against Staphylococcus chromogenes and Streptococcus uberis for dairy mastitis : https://doi.org/10.12982/VIS.2026.007. Veterinary Integrative Sciences, 24(1), 1–12. retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/269256
Section
Research Articles

References

Abera, M., Elias, B., Aragaw, K., Denberga, Y., Amenu, K., Sheraw, D., 2012. Major causes of mastitis and associated risk factors in smallholder dairy cows in Shashemene, southern Ethiopia. Afr. J. Agric. Res. 7(24), 3513-3518.

Alencar, J., Matos, F., Machado, M., Craveiro, A., 1996. Essential oil from Astronium fraxinifolium Schott (Anacardiaceae) in different growth stages. J. Essent. Oil Res. 8, 177-178.

Aliaa Saad, A.K., 2012. Study of in vitro antibacterial activity of the essential oils of cloves (Syzygium aromaticum) and the effect of temperature on antibacterial activity. Euphrates J. Agric. Sci. 4(1), 15-19.

Batavani, R.A., Asri, S., Naebzadeh, H., 2007. The effect of sub-clinical mastitis on milk composition in dairy cows. Iran. J. Vet. 8(3), 205-211.

Boonyayatra, S., Chaisri, W., 2005. Incidence and prevalence of sub-clinical mastitis in smallholder dairy farms of Chiang Mai Province, Thailand. Vet. Integr. Sci. 2, 25-30.

Caneschi, A., Bardhi, A., Barbarossa, A., Zaghini, A., 2023. Plant essential oils as a tool in the control of bovine mastitis: an update. Molecules. 28(8), 3425.

Chassagne, F., Samarakoon, T., Porras, G., Lyles, J.T., Dettweiler, M., Marquez, L., Salam, A.M., Shabih, S., Farrokhi, D.R., Quave, C.L., 2020. A systematic review of plants with antibacterial activities: A taxonomic and phylogenetic perspective. Front. Pharmacol. 11, 586548.

Corona-Gómez, L., Hernández-Andrade, L., Mendoza-Elvira, S., Suazo, F.M., Ricardo-González, D.I., Quintanar-Guerrero, D., 2022. In vitro antimicrobial effect of essential tea tree oil (Melaleuca alternifolia), thymol, and carvacrol on microorganisms isolated from cases of bovine clinical mastitis. Int. J. Vet. Sci. Med. 10(1), 72-79.

Desissa, F., Makita, K., Teklu, A., Grace, D., 2012. Contamination of informally marketed bovine milk with Staphylococcus aureus in urban and peri-urban areas of Debre-Zeit, Ethiopia. Afr. J. Microbiol. Res. 6(29), 5852-5856.

Dhouioui, M., Boulila, A., Chaabane, H., Zina, M.S., Casabianca, H. (2016). Seasonal changes in essential oil composition of Aristolochia longa L. ssp. paucinervis Batt. (Aristolochiaceae) roots and its antimicrobial activity. Ind. Crops. Prod. 83, 301-306.

Figueirôa Ede, O., Nascimento da Silva, L.C., de Melo, C.M., Neves, J.K., da Silva, N.H., Pereira, V.R., Correia, M.T., 2013. Evaluation of antioxidant, immunomodulatory, and cytotoxic action of fractions from Eugenia uniflora L. and Eugenia malaccensis L.: Correlation with polyphenol and flavanoid content. Sci. World. J. 2013, 125027.

Guedri Mkaddem, M., Zrig, A., Ben Abdallah, M., Romdhane, M., Okla, M.K., Al-Hashimi, A., Alwase, Y.A., Hegab, M.Y., Madany, M.M.Y., Hassan, A.H.A., Beemster, G.T.S., AbdElgawad, H., 2022. Variation of the chemical composition of essential oils and total phenols content in natural populations of Marrubium vulgare L. Plants (Basel). 11(5), 612.

Jasmine, R., Selvakumar, B.N., Daisy, P., Ignacimuthu, S., 2010. Activity of Eugenia jambolana, an ethnomedical plant, against drug-resistant bacteria. Pharm. Biol. 48(4), 405-410.

Labhar, A., El-Mernissi, Y., Ahidar, N., Zouhri, A., Benamari, O., Siddique, F., Bashir, M., Salhi, A., Ahari, M., Ibenmoussa, S., Bourhia, M., Salamatullah, A.M., Elyoussfi, A., Amhamdi, H., 2024. Seasonal variations in the essential oil composition and biological activities of wild lavandula dentata L. Nat. Prod. Commun. 19(2), 1-14.

Lopes, T.S., Fontoura, P.S., Oliveira, A., Rizzo, F.A., Silveira, S., Streck, A.F., 2020. Use of plant extracts and essential oils in the control of bovine mastitis. Res. Vet. Sci. 131, 186-193.

Maia, J.G.S., da Silva, M.H.L., Andrade, E.H.A., Zoghbi, M.D.G.B., Carreira, L.M.M., 2002. Essential oils from Astronium urundeuva (Allemao) Engl. and A. Fraxinifolium Schott ex Spreng. Flavour Fragr. J. 17(1), 72-74.

Matias, E.F., Alves, E.F., Silva, M.K., Victoria Carvalhoa, R.A., Fernando, G.F., João Ferreiraa, V.A., Henrique Coutinho, D.M., João Silvaa, Jaime Ribeiro-Filhoa, José Costa, G.M., 2016. Seasonal variation, chemical composition and biological activity of the essential oil of Cordia verbenacea DC (Boraginaceae) and the sabinene. Ind. Crops. Prod. 87, 45-53.

Montironi, I.D., Cariddi, L.N., Reinoso, E.B., 2016. Evaluation of the antimicrobial efficacy of Minthostachy

s verticillata essential oil and limonene against Streptococcus uberis strains isolated from bovine mastitis. Rev. Argent. Microbiol. 48(3), 210-216.

Murugaiyan, J., Kumar, P.A., Rao, G.S., Iskandar, K., Hawser, S., Hays, J.P., Mohsen, Y., Adukkadukkam, S., Awuah, W.A., Jose, R.A.M., Sylvia, N., Nansubuga, E.P., Tilocca, B., Roncada, P., Roson-Calero, N., Moreno-Morales, J., Amin, R., Kumar, B.K., Kumar, A., Toufik, A.R., Zaw, T.N., Akinwotu, O.O., Satyaseela, M.P., van Dongen, M.B.M., 2022. Progress in alternative strategies to combat antimicrobial resistance: focus on antibiotics. Antibiotics (Basel). 11(2), 200.

Niorn, C., Manfred, G., Terry, C., Pattharaporn, S., 2012. Research and development plan for fruit wine production in Thailand using Makiang as a case study. Asian J. Agric. Food Sci. 5(1), 39-44.

Owolabi, M.S., Kazeem, W., Dosoky, N.S., Setzer, W.N., 2013. The leaf essential oil composition of Eugenia javanica from South West Nigeria and insecticidal activity against Sitophilus zeamais. African J. Plant Sci. Biotech. 7(1), 86-88.

Pangprasit, N., Srithanasuwan, A., Suriyasathaporn, W., Chaisri, W., 2021. Antibacterial properties of lauric acid in combination with organic acids against major pathogens causing dairy mastitis. Vet. Integr. Sci. 19(1), 37-44.

Rodriguez-Burbano, D., Quijano-Celis, C., Pino, J., 2010. Composition of the essential oil from leaves of Astronium graveolens Jacq grown in Colombia. J. Essent. Oil. Res. 22, 488-489.

Sharun, K., Dhama, K., Tiwari, R., Gugjoo, M.B., Iqbal Yatoo, M., Patel, S.K., Pathak, M., Karthik, K., Khurana, S.K., Singh, R., Puvvala, B., Amarpal, Singh, R., Singh, K.P., Chaicumpa, W., 2021. Advances in therapeutic and managemental approaches of bovine mastitis: A comprehensive review. Vet. Q. 41(1), 107-136.

Singh, J., Baghotia, A., Goel, S., 2012. Eugenia caryophyllata Thunberg (family myrtaceae): a review. Int. J. Res. Pharm. Biomed. Sci. 3(4), 1469-1475.

Skowron, K., Sękowska, A., Kaczmarek, A., Grudlewska, K., Budzyńska, A., Białucha, A., Gospodarek-Komkowska, E., 2019. Comparison of the effectiveness of dipping agents on bacteria causing mastitis in cattle. Ann Agric Environ Med. 26(1), 39-45.

Suksamrarn, A., Brophy, J.J., 1987. The volatile leaf oil of Eugenia javanica lamk. Flavour Fragr. J. 2(1), 37-40.

Suvanapakdee, A., Saisathit, R., Sutthimussik, S., 2012. Efficiency of lemongrass and citronella essential oils in inhibiting mastitis-causing pathogens in dairy cows: Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli. Khon Kaen Agr. J. 40(2), 230-235.

Tancharoen, K., Sornnam, E., Panneam, S., 2007. Effects of plant extracts on the antibacterial activity AgainstGram-Positive bacteria, the cause of mastitis. In Proceedings of 45th Kasetsart University annual conference: animals and veterinary medicine, pp. 597-602.

Tina, P., Padmavath, D., Jasmin Sajini, R., Sarala, A., 2011. Syzygium Samarangense: a review on morphology, phytochemistry & pharmacological aspects. Asian J. Biochem. Pharm. Res. 4(1), 155-163.

Tomanić, D., Božin, B., Kladar, N., Stanojević, J., Čabarkapa, I., Stilinović, N., Apić, J., Božić, D.D., Kovačević, Z., 2022. Environmental bovine mastitis pathogens: Prevalence, antimicrobial susceptibility, and sensitivity to Thymus vulgaris L., thymus Serpyllum L., and Origanum vulgare L. essential oils. Antibiotics (Basel). 11(8), 1077.