Isolation and selection of probiotic Lactobacillus strains from chicken intestinal tract: A potential solution for sustainable poultry production in Vietnam https://doi.org/10.12982/VIS.2025.046
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Abstract
The escalating demand for sustainable poultry production in Vietnam necessitates the exploration of alternatives to antibiotic growth promoters. This study investigated the probiotic potential of Lactobacillus strains isolated from the intestinal tract of healthy chickens. Thirty isolates were screened for their resilience to acidic and bile salt conditions, key characteristics for probiotic survival in the gastrointestinal tract. The strain LC16 demonstrated exceptional tolerance to both low pH and bile salts. The antimicrobial activity of the selected strains was evaluated against common poultry pathogens (E. coli, Staphylococcus aureus, and Salmonella sp.), and their antibiotic susceptibility was also assessed. Strain LC16 exhibited both potent antimicrobial activity and resistance to all tested antibiotics. Molecular identification through PCR and 16S rRNA gene sequencing confirmed LC16 as Lactobacillus farciminis. The identification of L. farciminis LC16, a strain possessing a combination of desirable probiotic traits, including robust antimicrobial activity, tolerance to challenging gastrointestinal conditions, and antibiotic resistance, highlights its potential as a promising candidate for further development as a probiotic for chickens.
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