Exploring the synergy of CRISPR-Cas9 and IVF for Precision Livestock Production: A Review of Modern Advancements https://doi.org/10.12982/VIS.2026.039
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Abstract
Livestock plays a crucial role in global agricultural economy. A sustainable livestock production is the key player contributing to food security. Reproduction plays a central role in ensuring a smooth Livestock production. The era of 1980s marked a significant cornerstone in Livestock reproduction by the incorporation of in vitro fertilization (IVF), making it easier to develop genetically superior offspring. The precision and efficiency of genetic modifications was further enhanced by the integration of IVF with CRISPR/Cas9 technology. The technological union of CRISPR-Cas9 genome editing and in vitro fertilization (IVF) has brought revolutionary changes to livestock biotechnology programs. The combined strategies produce accurate, fast genetic progress through robust enhancements in numerous livestock species. IVF has established itself as a tool for breeding better livestock for increased reproductive success but joining it with CRISPR-Cas9 allows breeders to manipulate embryonic genomes precisely. This study investigates the various implementation methods of this technology including manipulation in the genome through knockout (KO) or knock-in (KI) processes to generate disease-resistant variants combined with production improvements, milk-allergen reduction and creation of transgenic animal research models for pharmaceutical industries. The article examines technical developments improving embryo editing tools alongside multiplex gene modification methods and innovation in IVF protocols. This research surveys both ethical matters alongside the effects; genome-edited livestock will have on regulatory environments. The article identifies potential future trends in this dual-platform biotechnology system, highlighting its role as a critical tool for developing precision-oriented, sustainable animal husbandry
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