Effects of shallot (Allium ascalonicum) powder supplementation on carcass quality and lipid peroxidation in broiler chickens under heat stress conditions https://doi.org/10.12982/VIS.2026.045
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Abstract
This study investigated the effects of shallot powder (SP) supplementation on oxidative stress and carcass characteristics in broiler chickens under both normal and heat stress (HS) conditions. A complete randomized design was used to allocate 120 one-day-old Arbor Acre chicks into six groups (4 replicates of 5 broiler chickens), over a duration of 42 days: T1 (control, basal diet), T2 (basal diet + HS), T3 (2 g/kg SP), T4 (4 g/kg SP), T5 (2 g/kg SP + HS), and T6 (4 g/kg SP + HS). Malondialdehyde (MDA) concentrations were assessed on days 21, 28, 35, and 42. On day 42, the carcass weight, component weight, and abdominal fat content were assessed. During the trial, SP administration markedly decreased MDA levels, with T4 exhibiting the lowest values. Compared with T2, T6 resulted in significantly lower MDA levels (P = 0.000), indicating an antioxidant effect under heat stress. SP enhanced carcass attributes. Compared with T2, T4 and T6 presented markedly elevated hot carcass weights (2,072.5±19.6 g and 2,033.8±20.9 g, respectively) (P = 0.000, P = 0.000). The SP-supplemented groups presented increased weights of the breast, drumstick, thigh, and wing cuts, particularly at T4 and T6. Furthermore, SP reduced abdominal fat, with the lowest levels observed at T4 (3.12±0.32%) and T6 (3.53±0.41%). In conclusion, 4 g/kg shallot powder diminishes oxidative stress and enhances carcass quality, particularly at elevated temperatures. The potential of this natural antioxidant feed additive to increase the performance of heat-stressed broilers is emphasized.
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References
Abass, K.H., Atiyah, W.R., Mlaghee, M.A., 2022. Assessment of malondialdehyde level in broiler chickens suffering from chronic heat stress and fed on a diet supplemented with bioactive substances. Int. J. Health Sci. 6(S9), 1714–1720.
Adeyemo, A.E., Omoba, O.S., Olagunju, A.I., Josiah, S.S., 2022. Chemical composition, in vitro antioxidant properties, and phenolic profile of shallot (Allium ascalonicum L.)-enriched plantain biscuit. Bull. Natl Res. Cent. 46, 85.
Aditya, S., Ahammed, M., Jang, S.H., Ohh, S.J., 2017. Effects of dietary onion (Allium cepa) extract supplementation on performance, apparent total tract retention of nutrients, blood profile and meat quality of broiler chicks. Asian-Australas J. Anim. Sci. 30, 229–235.
Al-Ramamneh, D., 2018. Reduce heat stress in broiler by adding onion. Russ. Agric. Sci. 44, 92–96.
Bedrníček, J., Kadlec, J., Laknerová, I., Mráz, J., Samková, E., Petrášková, E., Hasoňová, L., Vácha, F., Kron, V., Smetana, P., 2020. Onion peel powder as an antioxidant-rich material for sausages prepared from mechanically separated fish meat. Antioxidants. 9, 974.
Bhawa, S., Morêki, J.C., Machete, J.B., 2023. Poultry management strategies to alleviate heat stress in hot climates: A Review. J. World Poult. Res. 13(1), 1-9.
Ding, X., Giannenas, I., Skoufos, I., Wang, J., Zhu, W., 2023. The effects of plant extracts on lipid metabolism of chickens — A review. Anim. Biosci. 36(5), 679–691.
Istiteh, R., Abuajamieh, M., Al-Qaisi, M., Abedal-Majed, M.A., Abdelqader, A., 2025. Impact of probiotic-supplemented water on the performance and physiological responses of broilers under normal and heat stress conditions. Vet. World. 18(4), 1059–1069.
Kurniawan, M.I., Sjofjan, O., Tistiana, H., 2025. Evaluation of proximate and flavonoid test of shallot peel (Allium ascalonicum L) nanoparticles as poultry feed additives. In: Novianti, I., Suyadi, S., Boyd, D., Bagoudou, A.F., ShikhMaidin, M., Prafitri, R., (Eds.), Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024), pp. 280–286.
Latimer, Jr., George W., 2023. Official methods of analysis of AOAC international. Oxford University Press, Oxford.
Lu, Q., Wen, J., Zhang, H., 2007. Effect of chronic heat exposure on fat deposition and meat quality in two genetic types of chicken. Poult. Sci. 86(6), 1059-1064.
Malematja, E., Manyelo, T.G., Ng’ambi, J.W., Nemauluma, M.F.D., Kolobe, S.D., 2023. Effects of onion extracts (Allium cepa) inclusion in diets on growth performance, carcass characteristics, and bone morphometric of broiler chickens. Anim. Biosci. 36, 1075–1082.
Moldovan, C., Frumuzachi, O., Babotă, M., Barros, L., Mocan, A., Carradori, S., Crişan, G., 2022. Therapeutic uses and pharmacological properties of shallot (Allium ascalonicum): a systematic review. Front. Nutr. 9, 1–34.
National Research Council, 1994. Nutrient requirements of poultry, 9th revised edition. National Academies Press, Washington, D.C.
Nawaz, A.H., Amoah, K., Leng, Q.Y., Zheng, J.H., Zhang, W.L., Zhang, L., 2021. Poultry response to heat stress: Its physiological, metabolic, and genetic implications on meat production and quality including strategies to improve broiler production in a warming world. Front. Vet. Sci. 8, 699081.
Oluwagbenga, E.M., Fraley, G.S., 2023. Heat stress and poultry production: a comprehensive review. Poult. Sci. 102(3), 103141.
Omar, A.E., Al-Khalaifah, H.S., Mohamed, W.A.M., Gharib, H.S.A., Osman, A., Al-Gabri, N.A., Amer, S.A., 2020. Effects of phenolic-rich onion (Allium cepa L.) extract on the growth performance, behavior, intestinal histology, amino acid digestibility, antioxidant activity, and the immune status of broiler chickens. Front. Vet. Sci. 7, 582612.
Palupi, R., Abrar, A., Lubis, F.N.L., Kurniati, Y., Nadia, B.I.O., 2023. Analysis of nutrient digestibility, hematological status, and blood malondialdehyde levels in broiler chickens given a combination of cassava tuber and Indigofera zollingeriana leaves flour as a source of energy in the ration. J. Anim. Health Prod. 11(3), 206–213.
Punyatong, M., Thapingkae, W., Srinual, O., Lamsaengkul, C., 2026. Effects of glutamine supplementation on performance, carcass traits, and oxidative stress markers in native crossbred chickens under heat stress conditions. Vet. Integr. Sci. 24(1), 1-9.
Shah, M., Chand, N., Khan, R.U., Saeed, M., Ragni, M., Tarricone, S., Laudadio, V., Losacco, C., Tufarelli, V., 2023. Mitigating heat stress in broiler chickens using dietary onion (Allium cepa) and ginger (Zingiber officinale) supplementation. S. Afr. J. Anim. Sci. 52(6), 811-818.
Singleton, V.L., Orthofer, R., Lamuela-Raventós, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth. Enzymol. 299, 152-178.
Sobanke, A.O., Aiyeola, A., Okwuonu, F.I., Nnaemeka, W.S., Ndubuisi, J.C., Udeoji, F.I., Adiele, J.N., 2025. Allium cepa L. as a natural antioxidant: Its efficacy in combating heat stress-induced physiological alterations. Hum. Nutr. Metab. 39, 200293.
Surasorn, B., Sukon, P., Sornplang, P., 2024. Effects of shallot (Allium ascalonicum) powder supplementation on growth, intestinal morphology, immune stimulation, and intestinal bacteria in broiler chickens. Vet. World. 17(9), 2338–2346.
Zaboli, G., Huang, X., Feng, X., Ahn, D.U., 2019. How can heat stress affect chicken meat quality? – a review. Poult. Sci. 98(3), 1551–1556.