Antifungal Susceptibility of Dermatophytes and Non-dermatophytes to Amphotericin B, Terbinafine HCL, Griseofulvin, Ketoconazole and Itraconazol at Institute of Dermatology

Authors

  • Kunyanat Krongboon Department of Preventive and Social Medicine, Faculty of Medicine, Chulalongkorn University
  • Pornchai Sithisarankul Department of Preventive and Social Medicine, Faculty of Medicine, Chulalongkorn University
  • Ariya Chindamporn Department of Microbiology, Faculty of Medicine, Chulalongkorn University
  • Sirida Youngchim Department of Microbiology, Faculty of Medicine, Chiang Mai University
  • Navaporn Worasilchai Department of Transfusion Medicine and Clinical Microbiology, Research Unit of Medical Mycology Diagnosis, Faculty of Allied Health Sciences, Chulalongkorn University
  • Patcharin Thammasit Department of Microbiology, Faculty of Medicine, Chiang Mai University
  • Siriporn Chongkae Department of Microbiology, Faculty of Medicine, Chiang Mai University

Keywords:

Dermatophytes, Non-dermatophyte, Antifungal

Abstract

Background: Superficial Fungal infections are showing an escalating resistance to existing antifungal drugs. Effectively choosing an antifungal treatment for a persistent infection relies on identifying the infectious organism(s) and conducting susceptibility testing of the organism(s) to antifungal medications. Objective: The study was aimed at evaluating the susceptibility of dermatophytes and non-Dermatophytes, isolated from patients at the Institute of Dermatology in Thailand, to antifungal agents including amphotericin B (AMP B), terbinafine HCL (TER), griseofulvin (GF), ketoconazole (KCZ), and itraconazole (ITR). Method: The research was conducted following the protocols of Clinical and Laboratory Standards Institute (CLSI), USA.       A total of 50 isolates were examined using ITS-PCR sequencing to identify fungal species, including 17 from tinea corporis/cruris, 6 from tinea pedis, and 27 from onychomycosis. Result: The minimal inhibitory concentration (MIC) of AMP B, TER, GF, KCZ, and ITR against dermatophytes ranged from 0.25-4, 0.015-16, 0.06-8, 0.03-8, and 0.007-4 µg/ml, respectively. The minimal fungicidal concentration (MFC) ranges of AMP B, TER, GF, KCZ, and ITR were 0.5-4, 0.015->16, 0.06-16, 0.03->16, and 0.015-8 µg/ml, respectively. For non-dermatophytes, the MIC of AMP B, TER, GF, KCZ, and ITR ranges were 0.003->16, 0.03->16, 16->64, 0.125->16, and 0.25->16 µg/ml, while the MFC ranged from 0.03->16, 0.03->16, 16->64, 0.125->16, and 0.25->16 µg/ml, respectively. Conclusion: The findings suggest that non-dermatophytes, particularly Neoscytalidium dimidiatum, necessitate higher antifungal concentrations than dermatophytes. Terbinafine HCL may be a suitable choice for treating tinea infections caused by both dermatophytes and non-dermatophytes. The correlation between MIC and clinical outcome still needs to be determined for optimal laboratory results interpretation.

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Published

15-09-2024

How to Cite

1.
Krongboon K, Sithisarankul P, Chindamporn A, Youngchim S, Worasilchai N, Thammasit P, Chongkae S. Antifungal Susceptibility of Dermatophytes and Non-dermatophytes to Amphotericin B, Terbinafine HCL, Griseofulvin, Ketoconazole and Itraconazol at Institute of Dermatology. J DMS [Internet]. 2024 Sep. 15 [cited 2024 Oct. 8];49(3):50-9. Available from: https://he02.tci-thaijo.org/index.php/JDMS/article/view/265921

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