Comparative study of sperm cryopreservation between solid surface vitrification and vapor freezing technique
Keywords:
Cryopreservation, Human sperm, Solid Surface VitrificationAbstract
The present study aims to compare the effect of Solid Surface Vitrification and VaporFreezing compare sperm motility, sperm progressive motility, normal sperm morphology and vitality.52 semen samples from sperm donors were rated according to the World Health Organization criteria (WHO, 2010). Semen were sperm prepared by Discontinuos Density Gradient and divided into 2 groups and freezed by Solid Surface Vitrification and Vapor Freezing. Afterwards, thawed for evaluation of sperm characters.
The results showed better significance in sperm motility (42.85 ± 17.320% VS 36.53 ± 17,352%), sperm progressive motility (34.16 ± 17.08% VS 31.20 ± 17.22%) and normal sperm morphology (13.86 ± 5.583% VS 9.98 ± 5.599%) in Solid Surface Vitrification when compared to Vapor Freezing respectively whereas, sperm vitality obtained from Vapor Freezing appeared to be higher significantly when compared to Solid Surface Vitrification (72.75 ± 12.317% VS 68.96 ± 12.508%). Solid Surface Vitrification showed better results when compared to those of Vapor Freezing.
References
ธีระพร วุฒยวนิช. (2552). การแช่แข็งแบบเนื้อแก้ว. เชียงใหม่: บริษัทสันติภาพแพ็คพริ้นท์ จำกัด. น.108
ธีระพร วุฒยวนิช. (2552). การแช่แข็งแบบเนื้อแก้ว. เชียงใหม่:บริษัทสันติภาพแพ็คพริ้นท์ จำกัด.น.109
ธีระพร วุฒยวนิช. (2552). การแช่แข็งแบบเนื้อแก้ว. เชียงใหม่: บริษัทสันติภาพแพ็คพริ้นท์ จำกัด. น.161
สมบูรณ์ คุณานิคม. (2545). ภาวะมีบุตรยากและเทคโนโลยีช่วยการเจริญพันธุ์. กรุงเทพฯ: บริษัท พี.เอ.ลีฟวิ่ง จำกัด.
สุญาณี เวสสบุตร, สืบค้นเมื่อวันที่ 15มกราคม
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Gao DY, Liu J, Liu C, Mcgann LE, Watson PF, Kleinhans FW, Mazur P, Critser JK. (1995). Prevention of osmotic injury to human spermatozoa during addition and removal of glycerol. Hum Reprod. 10:1109-1122.
Ghasem S, Fakher R, Majied JZ. (2009).
Vitrification of small volume of normal human sperms: Use of open pulled straw carrier. J. Med. Sci, 9(1), 30-35.
Hammadeh M, Greiner S, Rosenbaum P, Schmidt W. (1999). Comparison Between Human Sperm Preservation Medium and TEST-Yolk Buffer on Protecting Chromatin and Morphology Integrity of Human Spermatozoa in Fertile and Subfertile Men After Freeze-Thawing Procedure. Journal of Andrology. published online, 22(6), 1012-1018.
Isachenko E, Isachenko V, Katkov II, Dessole S,
Nawroth F. (2003). Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success. Reproductive BioMedicine Online, 6(2), 191-200.
Kasai M, Mukaida T. (2004). Cryopreservation of
animal and human embryos by vitrification. Reproductive BioMedicine Online, 9(2), 164-170.
Moskovtsev SI, Lulat A. G-M, Librach CL. (2011). Cryopreservation of human spermatozoa by vitrification vs. slow freezing : Cannadian eaperience. In :Current Frontiers in Cryobiology. Agricultural and Biological Sciences, (3), 78-100.
Nawroth F, Isachenko V, Dessole S, Rahimi G, et al. (2002). Vitrification of Human Spermatozoa Without Cryoprotectants. Pubishing technology, 23(2), 93-102.
Satirapod C, et al. (2011).Comparison of
cryopreserved human sperm from solid surface vitrification and standard vapor freezing method: on motility, morphology, vitality and DNA integrity. J Andrology, 44(1), 786-790.
World Health Organization. (2010). WHO
Laboratory Manual for the Examination and Processing of Human Semen, 5th ed.
