Specimen submission in Ophthalmic pathology



specimen handling, ophthalmic pathology, fixation, specimen submission


Pathological examination has an essential role in disease diagnosis and prognosis. However, pathological interpretation may be difficult or even impossible if cellular morphology or microstructures are distorted due to an artifact, an artificial structure or tissue alteration caused by the preparation process for a microscopic slide. Ophthalmologists who submit specimens for pathological examination should have knowledge of specimen collection for surgical pathology, cytology, and frozen sections starting from the proper surgical techniques for each organ collection, type and amount of fixative, and container. Importantly, precise communication between a surgeon and a pathologist should be made via an ophthalmic pathology consultation request form. Data should be completely provided for the right understanding of the submitted specimen. This leads to an accurate interpretation of pathological results, which helps guide further patient management. 


Syed N, Berry J, Heegaard S, et al. Ophthalmic pathology and intraocular tumors. China: American Academy of Ophthalmology;2022:19-27.

Folberg R, Chévez-Barrios P, Lin A, et al. Tumors of the Eye and Ocular Adnexa. 5th ed. Korea: American Registry of Pathology;2021:1-20

Taqi SA, Sami SA, Sami LB, et al. A review of artifacts in histopathology. J Oral Maxillofac Pathol. 2018;22(2):279.

Singh R, Joseph A, Umapathy T, et al. Impression cytology of the ocular surface. Br J Ophthalmol. 2005;89(12):1655-1659.

Laver NMV. Ocular cytology: Diagnostic features and ongoing practices. Cancer Cytopathol. 2021;129(6):419-431.

Tseng SC. Staging of conjunctival squamous metaplasia by impression cytology. Ophthalmology. 1985;92(6):728-733.

วสี ตุลวรรธนะ. ตำราจักษุพยาธิวิทยา. ภูเก็ต: เวิลด์ออฟเซ็ท; 2552.25-54

ภาควิชา/ฝ่ายพยาธิวิทยา คณะแพทยศาสตร์ จุฬาลงกรณ์มหาวิทยาลัยและโรงพยาบาลจุฬาลงกรณ์ สภากาชาดไทย. คู่มือการส่งตรวจทางพยาธิวิทยา. 2563






Review Article