Cultured Epidermal Autograft in Wound coverage
คำสำคัญ:
Cultured epidermal autograft, CEA, Wound coverageบทคัดย่อ
Cultured epidermal autograft (CEA) was performed in 1975 and developed the technique in result of large sheets of multi-layer keratinocytes. Limitations are need of dermal layer support, take time of culture process and high expense. Consider cost-benefit compare to skin graft harvest disadvantage, cultured human epidermal keratinocytes should be gold standard treatment in skin loss wound closure in the future.
เอกสารอ้างอิง
Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975; 6(3): 331-343.
Brychta P, Suchanek I, Rihova H, Adler J, Komarkova J. Cultured epidermal allografts for the treatment of deep dermal burns. Acta Chir Plast 1995;37: 20–24.
Middelkoop E, van den Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MM. Porcine wound models for skin substitution and burn treatment. Biomaterials 2004;25: 1559–1567
Green H, Kehinde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci U S A. 1979;76(11):5665‐5668.
Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell. 1975;6(3):331‐343
Pruniéras M, Régnier M, Woodley D. Methods for cultivation of keratinocytes with an air-liquid interface. J Invest Dermatol. 1983; Jul811; Suppl 28-33.
Rosdy M, Clauss LC. Terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface. J Invest Dermatol. 1990; Oct954:409-14.
O’Connor NE. Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1981;1: 75–8.
Svensjo T, Yao F, Pomahac B, Eriksson E. Autologous keratinocyte suspensions accelerate epidermal wound healing in pigs. J Surg Res 2001;99: 211–21.
L Frese, Darwiche SE, Gunning ME, Hoerstrup SP, von Rechenberg B, Giovanoli P, Calcagni M. Optimizing large‐scale autologous human keratinocyte sheets for major burns—Toward an animal‐free production and a more accessible clinical application. Health Sci Rep. 2022 Jan; 5(1): e449.
Karlsson M, Steinvall I, Olofsson P, Thorfinn J, Sjöberg F, Åstrand L, Fayiz S, Khalaf A, Divyasree P, El-Serafi AT, Elmasry M. Sprayed cultured autologous keratinocytes in the treatment of severe burns: a retrospective matched cohort study. Ann Burns Fire Disasters. 2020 Jun 30; 33(2): 134–142
Barret JP, Wolf SE, Desai MH, Herndon DN. Cost-Efficacy of Cultured Epidermal Autografts in Massive Pediatric Burns. Ann Surg. 2000 Jun; 231(6): 869–876.
McAree KG, Klein RL, Boeckman CR. The use of cultured epithelial autografts in the wound care of severely burned patients. J Pediatr Surg 1993; 28:166–168.
