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Catheter-related bloodstream infections (CRBSIs) pose significant health risks and financial consequences for healthcare systems worldwide. In order to develop novel antiseptic technologies as alternative prophylactics for CRBSIs, a consistent and reproducible bacterial inoculation method is required for testing the technologies’ antiseptic effects. This study developed and investigated a new “Inocap” model for standardized bacteria inoculation on to surfaces of catheter hubs that is exposed and highly susceptible to contamination in healthcare settings. The results showed a significantly smaller variance in the number of bacteria inoculated onto the catheter hubs by the new Inocap method than the traditional dip method (p ≈ 0.02) while producing no significant difference in the mean value (p > 0.25), indicating that this new Inocap method could work as a more precise inoculation model for future disinfection studies.
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