ผลการเปรียบเทียบคุณลักษณะของเซลล์ลิมโฟซัยท์ในน้ำไขสันหลังปลอมที่เตรียมด้วยวิธีต่างๆ

Abstract

Objective: To study the three method of preparation of artificial cerebrospinal fluid and observe the bacterial contamination

after performing each technique. Materials and methods: Preparation lymphocyte cell from EDTA blood

with  high lymphocyte high lymphocyte discarded after HLA typing and apply lymphoproliferation assay ,three

methods wererinsed bysolution and centrifuged with any rpm. To get lymphocyte cell.The lymphocytes cell from

three  methods were counted by three technicians and mixed to neutrophils cell according to case study.The lymphocytes

and neutrophils were smeared for differentiation. Result: The comparison of three methods to preparation

lymphocytes  cell showed that three techniques yielded are no significant difference for the lymphocyte cell count (pvalue

≤ 0.05) but the lymphoproliferation assay provided no bacterial contamination and easy to preparation in one

day. Conclusion: Artificial cerebrospinal fluid prepared by provided higher lymphocyte count with no  bacterial contamination

better than the two methods and distinct morphology in Wright stain. So this technique could  be used as  learning and teaching material.