In vitro Effect of Ethanol on Erythrocyte Methemoglobin and Reduced Glutathione Concentrations
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Abstract
Background: Ethanol is well known as oxidative stress inducing factor. Many studies demonstrated that after alcohol intaking both in experimental animals and human, it has an impact on erythrocytes. Objectives: To study effect of ethanol (EtOH) on Methemoglobin (MetHb) and glutathione (GSH) levels in erythrocytes after in vitro exposure. Materials & Methods: Erythrocytes from 20 adult human subjects were treated with 25, 37.5, 50 and 62.5 mg/dL of ethanol (EtOH) as oxidant agent. We compared Methemoglobin (MetHb) and glutathione (GSH) levels in erythrocytes before and after exposure to EtOH. Results: Increasing of EtOH concentration and incubation time caused increasing of MetHb levels (p < 0.05). We found that MetHb levels in post-incubated erythrocytes were higher than those in untreated erythrocytes from three to ten times. Moreover, our results were demonstrated that high EtOH concentrations and long incubation time resulted in reducing of GSH levels in erythrocytes (p < 0.05). GSH levels in post-incubated erythrocytes were lower than those in untreated erythrocytes ranging from 0.32 to 8.14 mg/dL. Conclusions: EtOH as oxidative damage indicator induced MetHb formation and GSH depletion in vitro.
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