The genotypic detection of blaVIM-2 among clinical carbapenem resistant Pseudomonas aeruginosa isolates from Phramongkutklao hospital Thailand
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Abstract
This study aimed to determine the presence of Verona integron-encoded metallo-β-lactamase-2 (VIM2) gene among clinical Pseudomonas aeruginosa isolates. All studied isolates from patients admitted at Phramongkutklao Hospital, Bangkok, Thailand in 2009. The included P. aeruginosa isolates for this study were imipenem or meropenem non-susceptible strains according to Clinical and Laboratory Standards Institute. The antimicrobial susceptibility testing was determined by using disk diffusion. VIM2-gene detection was assessed by polymerase chain reaction technique. Three hundred and three carbapenem non-susceptible P. aeruginosa isolates (CNS-PA) were included. One hundred and sixty two were detected metalo-betalactamase production. Thirty-eight out of 303 CNS-PA isolates (12.54%) harboring blaVIM2 gene with detecting phenotypic production of metalo-betalactamase. Thus, blaVIM2 gene partially plays a role for carbapenem resistance, however the most studied CNS-PA isolates with no studied genes have to be further investigated the mechanisms being responsible for carbapenem resistance.
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