Antioxidant activity and total phenolic compounds of herbs and traditional recipes to reduce complications of diabetes

  • Ganniga Pumthong Faculty of Thai Traditional and Alternative Medicine
  • Jintana Junlatat Faculty of Thai Traditional and Alternative Medicine
  • Pitchanan Thiantongin Faculty of Thai Traditional and Alternative Medicine
  • Saran Chaweerak Faculty of Thai Traditional and Alternative Medicine
Keywords: antioxidant, phenolics, diabetes, herbs


This research aims to study the basic properties of herbs and folk remedies for the selection of herbs that have the potential to be developed as products to reduce the complications in diabetic patients. The present study investigated the in vitro antioxidant activities that helps inhibit free radicals causing cell damage and insulin resistance, as well as phenol content of herbs. This substance is associated with antioxidant activity and the actions to stimulate insulin releasing to uptake glucose from blood circulation into the cells.

          Two of antidiabetic traditional recipes included one from Kudchum hospital and another from Thai traditional medicine textbook, as well as four indigenous Thai herbs, such as leaves of mango, pomegranate, Tui, and Kradone. They were extracted with water and 50% ethanol. The extracts were examined two methods of antioxidant activity which were DPPH and FRAP. They are different in antioxidant properties. The phenol content was determined by the Folin-Ciocalteu reagent. The result showed that the decoction remedy contained Mallotus repandus, Azadirachta indica, and Tinospora crispa (75: 20: 5) gave a high antioxidant activity and polyphenol content. The remedy was suitable to be developed into a herbal product that reduces the complications of diabetes. When studying the composition of substances, it found that the effects of the components in three herbs were very appropriate to be developed further because of the mechanism of actions from the components in these herbs that can help reduce blood sugar level and complications of diabetes.


Download data is not yet available.


Chawla, D., Bansal, S., Banerjee, BD., et al. (2014). Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications. Microvascular Research. 95, 1–6.

Faradianna E. Lokman, Harvest F. Gu, Wan Nazaimoon, Wan Mohamud, et al. (2013). Evidence-Based Complementary and Alternative Medicine. V. 2013, Article ID 727602: 1-7.

Hasan, MM., Uddin, N., Hasan, MR., et al. (2014). Analgesic and Anti-Inflammatory Activities of Leaf Extract of Mallotus repandus (Willd.) Muell. Arg. BioMed Research International. V. 2014: 1-7.

K. Satyanarayana, K. Sravanthi2, I. Anand Shaker, & R. Ponnulakshmi. (2015). Molecular approach to identify antidiabetic potential of Azadirachta indica. Journal of Ayurveda & Integrative Medicine. 6(3): 165-174.

Langrand J, Regnault H, Cachet X, Bouzidi C, Villa AF, Serfaty L, Garnier R, & Michel S. (2014). Toxic hepatitis induced by a herbal medicine: Tinospora crispa. Phytomedicine. 21(8-9):1120-1123.

Lin JM, Lin CC, Chen MF, Ujiie T, & Takada A. (1995). Scavenging effects of Mallotus repandus on active oxygen species.
J Ethnopharmacol. 46(3):175-81.

Mao, JT., Xue, B., Smoake, J., Lu, Q Y., Park, H., et al. (2016). MicroRNA-19a/b mediates grape seed procyanidin extract-induced anti-neoplastic effects against lung cancer. Journal of Nutritional Biochemistry, 34, 118–125.

Md. Rakib Hasan, Nizam Uddin, Md. Monir Hossain, et al. (2014). In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of ethyl acetate extract of Mallotus repandus (Willd.) Muell. stem in rat model. Journal of Coastal Life Medicine. 2(9):721-726.

Mohammad A. & Alzohairy. (2016). Therapeutics Role of Azadirachta indica (Neem) and Their Active Constituents in Diseases Prevention and Treatment. Evidence-Based Complementary and Alternative Medicine. V. 2016, Article ID 7382506, 1-11.

Mukherjee, S., Phatak, D., Parikh, J., et al. (2012). Antiglycation and antioxidant activity of a rare medicinal orchid Dendrobium aqueum Lindl. Medicinal Chemistry & Drug Discovery. 2(2): 46-54.

Pumthong G, Nathasonb, A., Tuseewan, M., et al. (2015). Complementary and alternative medicines for diabetes mellitus management in ASEAN countries. Complementary Therapies in Medicine. 23: 617—625.

Ramkissoon, J.S., Mahomoodally, M.F., Ahmed, N., & Subratty. AH. (2013). Antioxidant and anti-glycation activities correlates with phenolic composition of tropical medicinal herbs. Asian Pacific Journal of Tropical Medicine. 6(7): 561-9.

Thomas A, Rajesh EK, & Kumar DS. (2016). The Significance of Tinospora crispa in Treatment of Diabetes Mellitus. Phytother Res. 30(3): 357-66.

Unoki, H., Bujo, H., Yamagishi, S., et al. (2007). Advanced glycation end products attenuate cellular insulin sensitivity by increasing the generation of intracellular reactive oxygen species in adipocytes. Diabetes Research and Clinical Practice. 76, 236–244.

Wu, C., Huang, S., Lin, J., & Yen, G. (2011). Inhibition of advanced glycation end product formation by foodstuffs. Food and Function. 2, 224–234.

Yildirim, A., Mavi, A. & Kara, A.A. (2001). Determination of antioxidant and antimicrobial activities of Rumexcrispus L. extracts. Journal of Agricultural and Food Chemistry. 49 (8): 4083-4089.

Yuan, H., Zhu, X., Wang, W., Meng, L., Chen, D., & Zhang, C. (2016). Hypoglycemic and anti-inflammatory effects of sea buckthorn seed protein in diabetic ICR mice. Food & Function. 7, 1610–1615.
How to Cite
Pumthong, G., Junlatat, J., Thiantongin, P., & Chaweerak, S. (2019). Antioxidant activity and total phenolic compounds of herbs and traditional recipes to reduce complications of diabetes. UBRU Journal for Public Health Research, 8(1), 8-16. Retrieved from