การพัฒนาผลิตภัณฑ์ต้นแบบน้ำยาบ้วนปากจากสารสกัดรากกัญชา การทดสอบความคงตัว และพิษเฉียบพลัน

Kanittha  Nakkliang; Thaval  Rerksngarm; Vanvisa  Sresumatchai; ์ืี์Nutnicha Tipparat; Pimphanit  Laungchaisin; Wikit  Prakaiharn

Authors

Kanittha Nakkliang Faculty of Public Health, Valaya Alongkorn University under the Royal Patronage Pathum Thani Province.
Thaval Rerksngarm Department of Medical Technology, College of Allied Health Sciences, Rattana Bundit University
Vanvisa Sresumatchai Department of Family health Faculty of Public ,Health, Mahidol University
์ืี์Nutnicha Tipparat Alternative Medicine Division, Department of Thai Traditional and Alternative Medicine, Ministry of Public Health
Pimphanit Laungchaisin Alternative Medicine Division, Department of Thai Traditional and Alternative Medicine, Ministry of Public Health
Wikit Prakaiharn Alternative Medicine Division, Department of Thai Traditional and Alternative Medicine, Ministry of Public Health

Keywords:

cannabis root extract, mouthwashes, acute toxicity, product development, stability test

Abstract

Cannabis sativa subsp. indica has been used for various purposes, especially for medical use. In the past, cannabis root has been used as an ingredient in mouthwash recipes to treat periodontitis. Objectives: This study aimed to develop prototype mouthwash formulations containing cannabis root extract and to evaluate their physical stability and acute toxicity. Materials and Method: the formulation extract, including four ingredients in equal proportions; Cannabis sativa, Smilax corbularia, Smilax Glabra and Syzygium aromaticum were extracted by decoction (water extract) and followed by freeze-drying. Mouthwash formulations with various extract concentrations were prepared. Physical stability; appearance, sedimentation, color, pH, and viscosity were evaluated after exposure to accelerated conditions (Heating-Cooling Cycles) and during storage at 30 °C for 3 months, compared to freshly prepared samples (Day 0). Acute toxicity was evaluated using the brine shrimp lethality assay (Artemia salina). Data were analyzed using descriptive statistic.

Results:Nine formulations of mouthwash products containing cannabis root extract were developed. Among these, Formulations 2, 5, and 8, with extract concentrations of 0.5, 1.0, and 1.5 mg/mL, respectively, were selected as suitable candidates for stability studies. The freshly prepared samples and those stored under real-time storage conditions at 30 °C for 3 months exhibited similar physical characteristics, including clear appearance with yellow, light brown, no sedimentation. pH ranged from 6.15 to 6.80, and viscosity ranged from 4.95 to 5.35 cP. Under accelerated storage conditions (heating–cooling cycles), reductions in both pH and viscosity were observed. Acute toxicity tests revealed low toxicity against brine shrimp, with LC₅₀ values between 501.12 and 820.24 µg/mL. Conclusion: The three selected mouthwash formulations containing cannabis root extract demonstrated good stability upon preparation and after storage at 30 °C for 3 months.

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