Evaluation of Complete Blood Count Parameters between Two Automated Hematology Analyzers; Coulter STKS and Sysmex SF3000

Abstract

Evaluation of Complete Blood Count Parameters between Two Automated Hematology Analyzers; Coulter STKS and Sysmex SF3000

Boonpa     Yodsowan      BSc (Med Tech), MSc (Biochemistry)

Department of Cantral Laboratory, BMA Medical College and Vajira Hospital

Objective : To compare CBC parameters obtained from two different automated hematology analyzers; Coulter STKS and Sysmex SF3000

Study design : Cross-sectional analytical study.

Subjects : One hundred and twenty normal bold samples from normal healthy subjects who came to hospital for blood donation and check up including patient samples who requested for complete blood count during February 12, 2001 -March 30, 2001.

Methods : Blood specimens were collected from those normal subjects and patients. CBC were performed and all parameters obtained were studied for their relationships.

Main outcome measures : The amounts of hemoglobin, hematocrit, red bold call count, white blood cell count and the percentage of white bold cell differential were compared.

Results : Data from the two methods were statistically calculated. The correlation studies of all parameters including hemoglobin, hematocrit , blood cell count, white blood cell count and the percentage of neutrophil, lymphocyte, eosinophil, monocyte and basophil showed good correlation, the correlation coefficient ( r ) were 0.993, 0.974, 0.995, 0.993, 0.991, 0.991. 0.995, 0.901 and 0.575, respectively. Other findings showed significant statistical differences (p < 0.05) of all parameters except the differential of basophil showed no significant statistical differences (p › 0.05).

Conclusion : Utilization of the Sysmex SF3000 as a back up instrument to the Coulter STKS, the correlative study of parameters obtained from these two instruments was required. This study showed good correlation of all parameters by using regression analysis and significant statistical differential of basophil showed no significant statistical different by paired-t-test.

Kay words : complete blood count, automated hematology analyzer, Coulter STKS, Sysmez SF3000

Vajira Med J 2002 ; 46 : 59 - 65