The Detection of Opisthorchis viverrini DNA in Bile Samples from Obstructive Jaundice Patients by Real-time PCR

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Published: Jun 1, 2018
Keywords:
Bile Opisthorchis viverrini Obstructive jaundice Real-time PCR Conventional PCR

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Nithikoon Aksorn
Department of Clinical Pathology, Faculty of Medicine Vajira hospital. Navamindradhiraj University. Bangkok, Thailand
Sittiruk Roytrakul
Proteomics Research Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani, Thailand.
Kawin Leelawat
Department of Surgery, Rajavithi Hospital, Bangkok, Thailand.
Shinjiro Hamano
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan
Sundusit Pengsa
Department of Surgery, Udon Thani Cancer Hospital, Udon Thani, Thailand
Namthip Munponsri
Department of diagnostic radiology, Udon Thani Cancer Hospital, Udon Thani, Thailand
Usa Lek-Uthai
Department of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Bangkok, Thailand

Abstract

Objective: To detect Opisthorchis viverrini (O. viverrini) DNA in bile samples from obstructive jaundice patients by real-time PCR assay and to compare the results of real-time PCR with conventional PCR.


Methods: Bile samples were collected from 43 patients with obstructive jaundice from the Department of Surgery, Rajavithi Hospital and the Department of Surgery and Department of Diagnostic Radiology, Udon Thani Cancer Hospital. Twenty-eight cholangiocarcinoma (CCA) and 15 non-CCA patients underwent endoscopic retrograde cholangiography (ERCP) and biliary tract surgery. All bile samples were detected for O. viverrini DNA by real-time PCR and conventional PCR. The results of positive and negative bile samples for O. viverrini DNA detection by real-time PCR and conventional PCR detection were compared.


Results: Thirteen (30.2%) out of 43 bile samples were O. viverrini DNA positive. Nine of 13 bile samples were positive by real-time PCR and conventional PCR, while 4 bile samples were positive only by real-time PCR. Thirty bile samples (69.8%) were negative for O. viverrini DNA by both methods. The sensitivity, specificity, positive and negative predictive values of the real-time PCR were 100%, 88.2%, 69.2% and 100%, respectively.


Conclusion: Real-time PCR assay is suitable for detection of O. viverrini DNA in bile samples especially in obstructive jaundice patients.

Article Details

How to Cite
Aksorn, N., Roytrakul, S., Leelawat, K., Hamano, S., Pengsa, S., Munponsri, N., & Lek-Uthai, U. (2018). The Detection of Opisthorchis viverrini DNA in Bile Samples from Obstructive Jaundice Patients by Real-time PCR. Vajira Medical Journal : Journal of Urban Medicine, 62(3), 159–168. Retrieved from https://he02.tci-thaijo.org/index.php/VMED/article/view/195229
Issue
Vol. 62 No. 3 (2018): MAY - JUN 2018
Section
Original Articles

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