Anti-Proliferation and Anti-Migration Effect of a Medicinal Mushroom, Schizophyllum Commune, on Human Cholangiocarcinoma Cell Line
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Abstract
Objective: To investigate the possible anti-proliferation and anti-migration effect of the crude extracts from Schizophyllum commune against human cholangiocarcinoma cell line, KKU-M213
Methods: In this study, dried fruiting bodies of S. commune were extracted with 95% ethanol and water. Antiproliferative activity of the crude extracts from S. commune against human cholangiocarcinoma cell line, KKU-M213 was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, diphenyl tetrazolium bromide (MTT) assay. Wound healing assay was used for investigating the effects of the mushrooms crude extracts on KKU-M213 cell migration.
Results: The percentage yields of S. commune ethanol and water extracts were 5.42 % and 7.78 %, respectively. The results of MTT assay showed that 200 µg/ml of ethanol crude extracts significantly inhibited proliferation of KKU-M213 to 50% compared to untreated control. While hot water extracts of S. commune did not have any effects on KKU-M213 cell proliferation. Wound healing assay result showed that both hot water and ethanolic extracts of S. commune suppressed KKU-M213 cell migration.
Conclusion: This is the first report showing the anti-proliferation and anti-migration activity of this mushroom against human cholangiocarcinoma.
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References
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