รูปแบบผลการทดสอบความไวต่อสารต้านจุลชีพของเชื้อ Neisseria gonorrhoeae ในโรงพยาบาลอุตรดิตถ์ ระหว่างปี พ.ศ. 2561-2566

Wipha Noitachang

doi:10.1016/hscr.v39i1.268683

Authors

Wipha Noitachang Department of Medical Technology and Clinical Pathology, Uttaradit Hospital

DOI:

https://doi.org/10.1016/hscr.v39i1.268683

Keywords:

N. gonorrhoeae, gonorrhoeae, antimicrobial resistant, surveillance

Abstract

ABSTRACT
Objective: To study the antimicrobial susceptibility testing of N.gonorrhoeae isolates from patients was treated at Uttaradit hospital during 2018-2023
Methods: A total of 150 N. gonorrhoeae isolates from patients was treated at Uttaradit Hospital during October 2018 and September 2023. Antimicrobial susceptibility test was performed by the standard disk diffusion test and E-test method for azithromycin, ceftriaxone, cefixime, ciprofloxacin, penicillin, tetracycline, and spectinomycin. The data was analyzed by descriptive statistics such as average, percentage, standard deviation, range of minimal inhibitory concentration (MIC range), MIC₅₀ and MIC₉₀.
Results: It was found that the resistance rates of ciprofloxacin, tetracycline and penicillin were 98.0%, 94.0% and 92.0%, respectively. The prevalent rate of multi-drug resistance was also high (86.7%). All isolates were still susceptible to azithromycin with MIC range, MIC50 and MIC₉₀ of ≤0.016-0.25 mg/L, 0.125, and 0.25 mg/L, respectively. The isolates were also susceptible to ceftriaxone and cefixime with low MIC levels. The MIC range, MIC₅₀ and MIC₉₀ of ceftriaxone were ≤0.002-0.125 mg/L, 0.004 and 0.008 mg/L, respectively while the MIC range of cefixime was ≤0.016-0.064 mg/L and MIC₅₀ and MIC90 of <0.016 and 0.016 mg/L, respectively.
Conclusions: The N.gonorrhoeae isolates were not found decreased susceptibility to azithromycin, ceftriaxone and cefixime. This antimicrobial susceptibility surveillance in each hospital is needed for patient management and prevention and control of the emergence and spread of antibiotic-resistant in the future.
Keywords: N. gonorrhoeae, gonorrhoeae ,antimicrobial resistant, surveillance

References

Unemo M, Lahra MM, Cole M, et al: World Health Organization Global Gonococcal Antimicrobial SurveillanceProgram (WHO GASP): of new data and evidence to inform international collaborative actionsand research efforts. Sex Health 2019; 16(5): 412–25.

Center for Disease Control and Prevention (CDC): Sexually Transmitted Disease Surveillance 2019.[Internet].[cited 2023 october 30]. Available from: https://www. cdc.gov/std/statistics/ 2019/figures.htm.

รสพร กิตติเยาวมาลย์ และคณะ. แนวทางการดูแลรักษาโรคหนองใน พ.ศ. 2562. กรุงเทพฯ: สำนักพิมพ์อักษรกราฟฟิคแอนด์ดีไซน์; 2562 สำนักระบาดวิทยา กรมควบคุมโรค, รายงานโรค ในระบบเฝ้าระวัง 506: Gonorrhea 2561. [Internet] .[cited 2023 october30]. Available from: http://www.boe.moph.go.th/boedb/surdata/506wk/y61/d38_5261.pdf

ศูนย์เฝ้าระวังเชื้อดื้อยาต้านจุลชีพแห่งชาติ กรมวิทยาศาสตร์การแพทย์ (National Antimicrobial Resistance Surveillance, Thailand: NARST) สถาบันวิจัยวิทยาศาสตร์สาธารณสุข กรมวิทยาศาสตร์การแพทย์. ผลการทดสอบความไวต่อยาของเชื้อ Neisseria gonorrhoeae ปี 2012-2020. [Internet].[cited 2023 november 01]. Available from: http://narst.dmsc. moph.go.th/data/MIC-Neisseria_gonorrhoeae%202012-2020.pdf.

Buasakul P, Sangprasert P, Kittiyaowamarn R:Antimicrobial Susceptibility In N.gonorrhoeae IN 2012-2018 . Journal of AIDS.2020;32(2):42-53

พงศธร แสงประเสริฐ. โครงการเฝ้าระวังหนองในดื้อยาภาพรวมประเทศ ปี 2562–2564. กองโรคเอดส์และโรคติดต่อทางเพศสัมพันธ์ กรมควบคุมโรค กระทรวงสาธารณสุข

Kueakulpattana N, Wannigama DL, Luk-In S,Hongsing P, Hurst C, Badavath VN, et al. Multidrug-resistant Neisseria gonorrhoeae infection in heterosexual men with reduced susceptibility to ceftriaxone, first report in Thailand. Sci Rep. 2021;11(1):21659.

Kittiyaowamarn R, Girdthep N, Cherdtrakulkiat T, Sangprasert P, Tongtoyai J, Weston E, et al. Neisseria gonorrhoeae antimicrobial susceptibility trends in Bangkok,Thailand, 2015–21: Enhanced Gonococcal Antimicrobial Surveillance Programme (EGASP). JAC Antimicrob Resist. https://doi.org/10.1093/jacamr/dlad139

Maneerat S, Wilawan O,Brian V, Mariusz W, Samandra D, et al.Antimicrobial Susceptibility of Neisseria gonorrhoeae in Adult Patients Seeking Care at Military Hospitals in Thailand From 2014 to 2020.[Internet]. [cited 2024 june 29]. Available from: https://www.afrims.go.th/main/download/upload/ document-20220506 100907.pdf

Clinical and Laboratory Standards Institute (CLSI).Performance standards for antimicrobial and susceptibility testing:33th informational supplement. Document M100-S24 (M02-A11).Wayne, PA:CLSI;2023.