Effect of in vitro Anti-diabetic Activity of Cassia siamea Flowers and Leaves Extract

Authors

  • Thanakorn Damsud Program of Applied Biology, Faculty of Science and Technology, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat, 80110
  • Pensri Penprapai Innovation and Natural Product Research Unit, Faculty of Science and Technology,
  • Premjit Rongsawat Program of Science, Faculty of Science and Technology, Rajamangala University of Technology Srivijaya

Keywords:

Cassia siamea, , Anti-diabetic, Dipeptidyl peptidase IV inhibitor, Glucose diffusion, Alfa-Glucosidase inhibitor

Abstract

Background: Diabetes is a chronic metabolic disease usually caused by defects in insulin secretion. In addition, diabetes is a potential risk of developing the initiation of its complications, as a result of prolonged high blood glucose level. Objective: The aim of this work was to reduce blood glucose level potential of flowers and leaves extract from Cassia siamea through dipeptidyl peptidase IV (DPP-IV) inhibitor, gif.latex?\alpha-glucosidase inhibitory activity, glucose adsorption and glucose diffusion in vitro. Methods: The methanol crude extracts of flowers and leaves extract from C. siamea were evaluated for DPP-IV inhibitor, gif.latex?\alpha-glucosidase inhibitory activity from rat intestinal (matase and sucrase), glucose adsorption, glucose diffusion in vitro and preliminary of phytochemical screening. Results: Preliminary, phytochemical screening showed the presence of flavonoids, alkaloids, and phenolic compounds in flowers and leaves extract. In this study, the methanol extract of leaves showed stronger DPP-IV inhibitor and gif.latex?\alpha-glucosidase inhibitory activity (maltase and sucrase) with the IC50 values of 0.40, 3.77 and 3.64 mg/mL, respectively than flowers extract. Vildaglipin and acarbose, based on standard reference for DPP-IV and gif.latex?\alpha-glucosidase (maltase and sucrase) inhibitory activity, has IC50 values of 0.32, 0.36 and 0.97 mg/mL, respectively. In addition, the methanol extract of flowers and leaves showed most potent glucose adsorption and glucose diffusion retardation. Conclusion: Thus, the results from the studied methanol extracts flowers and leaves could be developed as the anti-hyperglycemic remedy for diabetes therapy.

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Published

2020-03-26

How to Cite

1.
Damsud T, Penprapai P, Rongsawat P. Effect of in vitro Anti-diabetic Activity of Cassia siamea Flowers and Leaves Extract . J Chulabhorn Royal Acad [Internet]. 2020 Mar. 26 [cited 2024 Jul. 1];2(1):36-49. Available from: https://he02.tci-thaijo.org/index.php/jcra/article/view/225064

Issue

Vol. 2 No. 1 (2020): January - March

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Research Articles

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