The remineralization quality by fluoridated dentifrice on artificial incipient caries lesion The remineralization quality by fluoridated dentifrice on artificial incipient caries lesion

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Nirin Manosubsak
Waleerat Sukarawan
Wannakorn Sriarj


Objectives: To evaluate the surface hardness, fluoride content, and topography of artificial incipient caries remineralized using fluoride dentifrice.

Methods: Human enamel specimens (n = 100) were randomly assigned to 4 groups: artificial saliva, 1,100-ppm, 1,450-ppm, and 5,000-ppm fluoride dentifrice (FD). Artificial incipient caries samples were immersed in fluoride dentifrice slurry twice for 2 min during a 10-day pH-cycling. The percentage of surface hardness recovery (%SMHR) was calculated. Topography was evaluated using scanning electron microscopy and fluoride content was analyzed by energy dispersive x-ray spectroscopy. The differences in the %SMHR and fluoride content were compared at the 95% confidence level.

Results: The significantly highest %SMHR and the highest fluoride content was found in the 5,000-ppm FD group. There was no significant difference in %SMHR or fluoride content between the 1,100-ppm and 1,450-ppm FD groups. The SEM image of the 5,000-ppm FD group specimens demonstrated a uniformed layer of densely packed calcium fluoride globules, while those of the 1,100-ppm and 1,450-ppm FD specimens were loosely packed.

Conclusions: The daily use of 5,000 ppm FD results in a homogenous scatter and densely pack of calcium fluoride globules while the regular use of 1,100 ppm and 1,450 fluoride dentifrice precipitates in a loosely cluster of calcium fluoride agglomerates. The difference in the pattern and amount of calcium fluoride may influence surface hardness.


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Manosubsak N, Sukarawan W, Sriarj W. The remineralization quality by fluoridated dentifrice on artificial incipient caries lesion: The remineralization quality by fluoridated dentifrice on artificial incipient caries lesion. M Dent J [Internet]. 2019 Apr. 29 [cited 2023 Dec. 9];39(1):53-62. Available from:
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