Effect of modified smear layer on the bond strength of all-in-one adhesives to dentin

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Suppason Thitthaweerat
Kadekaew Klinklao
Pisol Senawongse


            Objectives: To evaluate the effect of modified smear layer using EDTA, NaOCl solutions with and without agitating application to solution on the bond strength of all-in-one adhesives to dentin.

Materials and Methods: One hundred and two non-carious third molars were cut perpendicular to the long axis at 2 mm. above CEJ. The standardized smear layer on dentin surface was created by polishing with 600-grit carbide paper. Then, all specimens were randomly divided to 6  groups of 15 teeth: 1) Distilled water, 2) Distilled water with agitation, 3) 17% EDTA, 4) 17% EDTA with agitation, 5) 1% NaOCl, and 6) 1% NaOCl with agitation. One ml of each solution was applied on the dentin surface for 60 s. Smear layer characteristic was observed under SEM. Three all-in-one adhesives (SE-ONE: SE, G-ænial bond: GB, Optibond all-in-one: OP) were applied following the instructions. All specimens were restored with resin composite (Z250XT). The micro-tensile bond strength test and failure mode analysis were investigated. Smear layer characteristic was observed in each experimental group of two teeth using SEM.

Results: Modified smear layer using both solutions significantly increased the bond strength in SE and OP groups with p < 0.05. Agitation application could produce significantly higher bond strength in GB and SE (p < 0.05). The clean dentin surface with some erosion and opening of dentinal tubules were found when modified with EDTA. While, NaOCl exhibited the reduction in smear layer thickness and dense smear layer.

Conclusion: Modified smear layer with EDTA and NaOCl could gain the bond strength of some all-in-one adhesives (SE, OP). The agitation application to pretreated solutions improved the bonding performance of some all-in-one adhesives (SE, GB)


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Thitthaweerat S, Klinklao K, Senawongse P. Effect of modified smear layer on the bond strength of all-in-one adhesives to dentin. M Dent J [Internet]. 2018 Jan. 8 [cited 2023 Dec. 11];38(1):11-2. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/179230
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