Effect of modified smear layer on the bond strength of all-in-one adhesives to dentin

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Suppason Thitthaweerat
Kadekaew Klinklao
Pisol Senawongse


            Objectives: To evaluate the effect of modified smear layer using EDTA, NaOCl solutions with and without agitating application to solution on the bond strength of all-in-one adhesives to dentin.

Materials and Methods: One hundred and two non-carious third molars were cut perpendicular to the long axis at 2 mm. above CEJ. The standardized smear layer on dentin surface was created by polishing with 600-grit carbide paper. Then, all specimens were randomly divided to 6  groups of 15 teeth: 1) Distilled water, 2) Distilled water with agitation, 3) 17% EDTA, 4) 17% EDTA with agitation, 5) 1% NaOCl, and 6) 1% NaOCl with agitation. One ml of each solution was applied on the dentin surface for 60 s. Smear layer characteristic was observed under SEM. Three all-in-one adhesives (SE-ONE: SE, G-ænial bond: GB, Optibond all-in-one: OP) were applied following the instructions. All specimens were restored with resin composite (Z250XT). The micro-tensile bond strength test and failure mode analysis were investigated. Smear layer characteristic was observed in each experimental group of two teeth using SEM.

Results: Modified smear layer using both solutions significantly increased the bond strength in SE and OP groups with p < 0.05. Agitation application could produce significantly higher bond strength in GB and SE (p < 0.05). The clean dentin surface with some erosion and opening of dentinal tubules were found when modified with EDTA. While, NaOCl exhibited the reduction in smear layer thickness and dense smear layer.

Conclusion: Modified smear layer with EDTA and NaOCl could gain the bond strength of some all-in-one adhesives (SE, OP). The agitation application to pretreated solutions improved the bonding performance of some all-in-one adhesives (SE, GB)

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Thitthaweerat S, Klinklao K, Senawongse P. Effect of modified smear layer on the bond strength of all-in-one adhesives to dentin. M Dent J [Internet]. 2018 Jan. 8 [cited 2024 Jun. 25];38(1):11-2. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/179230
Original articles


1. Van Landuyt KL, Mine A, De Munck J, et al. Are one-step adhesives easier to use and better performing? Multifactorial assessment of contemporary one-step self-etching adhesives. J Adhes Dent 2009; 11: 175-90.
2. Belli R, Sartori N, Peruchi LD, et al. Effect of multiple coats of ultra-mild all-in-one adhesives on bond strength to dentin covered with two different smear layer thicknesses. J Adhes Dent 2011; 13: 507-16.
3. Mine A, De Munck J, Cardoso MV, et al. Dentin-smear remains at self-etch adhesive interface. Dent Mater J 2014; 30: 1147-53.
4. Suyama Y, Luhrs AK, De Munck J, et al. Potential smear layer interference with bonding of self-etching adhesives to dentin. J Adhes Dent 2013; 15: 317-24.
5. Torii Y, Hikasa R, Iwate S, Oyama F, Itou K, Yoshiyama M. Effect of EDTA conditioning on bond strength to bovine dentin promoted by four current adhesives. Am J Dent 2003; 16: 395-400.
6. Nikaido T, Takano Y, Sasafuchi Y, Burrow MF, Tagami J. Bond strengths to endodontically-treated teeth. Am J Dent 1999; 12(4):177-80.
7. Thanatvarakorn O, Nakajima M, Prasansuttiporn T, Ichinose S, Foxton RM, Tagami J. Effect of smear layer deproteinizing on resin-dentine interface with self-etch adhesive. J Dent 2014; 42: 298-304.
8. Kasraei S, Azarsina M, Khamverdi Z. Effect of Ethylene diamine tetra acetic acid and sodium hypochlorite solution conditioning on microtensile bond strength of one-step self-etch adhesives. J Conserv Dent 2013; 16: 243-6.
9. Kunawarote S, Nakajima M, Foxton RM, Tagami J. Effect of pretreatment with mildly acidic hypochlorous acid on adhesion to caries-affected dentin using a self-etch adhesive. Eur J Oral Sci 2011; 119: 86-92.
10. Nakajima M, Kunawarote S, Prasanauttiporn T, Tagami J. Bonding to caries-affected dentin. Jpn Dent Sci Rev 2011;47: 102-14.
11. Jacques P, Hebling J, Effect of dentin conditioners on the microtensile bond strength of a conventional and a self-etching primer adhesive system. Dent Mater 2005;21:103-9.
12. Chaves P, Giannini M and Ambrosano GM. Influence of smear layer pretreatments on bond strength to dentin. J Adhes Dent 2002; 4: 191-6.
13. Serper A, Calt S. The demineralizing effect of EDTA at different concentration and pH. J Endod 2002; 28: 501-2.
14. Calt S, Serper A. Time-dependent effects of EDTA on dentin structures. J Endod 2002; 28: 17-9.
15. Sen BH, Erturk O, Piskin B. The effect of different concentrations of EDTA on instrumented root canal walls. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108: 622-7.
16. Toledano M, Osorio R, Perdigao J, Rosales JI, Thompson JY and Cabrerizo-Vilchez MA. Effect of acid etching and collagen removal on dentin wettability and roughness. J Biomed Mater Res 1999; 47: 198-203.
17. Lai SC, Mak YF, Cheung GS, et al. Reversal of compromised bonding to oxidized etched dentin. J Dent Res 2001; 80: 1919-24.
18. Kunawarote S, Nakajima M, Shida K, Kitasako Y, Foxton RM, Tagami J. Effect of dentin pretreatment with mild acidic HOCl solution on microtensile bond strength and surface pH. J Dent 2010; 38: 261-8.
19. Thanatvarakorn O, Prasansuttiporn T, Thittaweerat S, Foxton RM, Ichinose S, Tagami J, Hosaka K, Nakajima M. Smear layer-deproteinizing improves bonding of one-step self-etch adhesives to dentin. Dent Mater. 2017 Article in press Dec 9. pii: S0109-5641(17)30537-7.
20. De Munck J, Vargas M, Iracki J, Van Landuyt K, Poitevin A, Lambrechts P, Van Meerbeek B. One-day bonding effectiveness of new self-etch adhesives to bur-cut emanel and dentin. Oper dent 2005; 30:39-49.
21. Tay FR, Pashley DH. Aggressiveness of contemporary self-etching systems. I: Depth of penetration beyond dentin smear layer. Dent Mater 2001; 17: 296-308.
22. Ermis RB, De Munck J, Cardoso MV, et al. Bond strength of self-etch adhesives to dentin prepared with three different diamond burs. Dent Mater 2008; 24: 978-85.
23. Cardoso MV, Coutinho E, Ermis RB, et al. Influence of dentin cavity surface finishing on micro-tensile bond strength of adhesives. Dent Mater 2008; 24: 492-501.
24. Kenshima S, Francci C, Reis A, Loguercio AD and Filho LE. Conditioning effect on dentin, resin tags and hybrid layer of different acidity self-etch adhesives applied to thick and thin smear layer. J Dent. 2006; 34: 775-83.
25. Thanatvarakorn O, Prasansuttiporn T, Takahashi M, et al. Effect of scrubbing technique with mild self-etching adhesives on dentin bond strengths and nanoleakage expression. J Adhes Dent 2016; 18: 197-204.
26. Senawongse P, Srihanon A, Muangmingsuk A and Harnirattisai C. Effect of dentine smear layer on the performance of self-etching adhesive systems: A micro-tensile bond strength study. J Biomed Mater Res B Appl Biomater 2010; 94: 212-21.
27. Loguercio AD, Stanislawczuk R, Mena-Serrano A and Reis A. Effect of 3-year water storage on the performance of one-step self-etch adhesives applied actively on dentine. J Dent 2011; 39: 578-87.
28. Takahashi A, Sato Y, Uno S. Effects of mechanical properties of adhesive resins on bond strength to dentin. Dent Mater 2002; 18: 263-8.
29. Hass V, Dobrovolski M, Zander-Grande C, et al. Correlation between degree of conversion, resin-dentin bond strength and nanoleakage of simplified etch-and-rinse adhesives. Dent Mater 2013; 29: 921-8.
30. Cho BH, Dickens SH. Effects of the acetone content of single solution dentin bonding agents on the adhesive layer thickness and the microtensile bond strength. Dent Mater 2004; 20: 107-15.
31. Van Landuyt KL, Snauwaert J, De Munck J, et al. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials 2007; 28: 3757-85.
32. Oysaed H, Ruyter IE. Water sorption and filler characteristics of composites for use in posterior teeth. J Dent Res 1986; 65:1315-8.
33. Yoshida Y, Nagakane K, Fukuda R, et al. Comparative study on adhesive performance of functional monomers. J Dent Res. 2004; 83: 454-8.
34. Garcia RN, Morelli AE, Da Silva BS, et al. Bonding performance of a self-adhering flowable composite to substrates used in direct technque. RSBO 2013;10: 343-9.