Antibacterial effect of herbal plants against three cariogenic microorganisms
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Published:
Dec 28, 2016
Keywords:
herbal extracts; oral microorganisms; antibacteria; Dracaena loureiri Gagnep.; Artocarpus lakoocha Roxb. (heartwood); Albizia myriophylla Benth.
Main Article Content
Abstract
Objective: Herbs have been recognized as therapeutic medicines that provide natural and safer remedies to dozens of common ailments for several centuries. This project evaluated the antimicrobial activity of Thai traditional herbal plants against three common oral bacteria. Methods: Forty-three methanol herbal extracts from thirty-three plants were selected for screening test. Herbal plants which exhibited antimicrobial properties were tested further via disc diffusion and broth microdilution assays. The bioactive compound was extracted from the herb that presented the highest antimicrobial properties and its antimicrobial effect was also tested by disc diffusion.
Results: Three herbal extracts from Dracaena loureiri Gagnep., Artocarpus lakoocha Roxb. (heartwood) and Albizia myriophylla Benth. revealed antimicrobial properties against Streptococcus mutans, Lactobacilllus casei, and Actinomyces viscosus with minimum bactericidal concentration (MBC) ranging from 0.58-9.37 mg/ml. Albizia myriophylla Benth. presented better bactericidal effect against three strains compared to other plants. However, A. lakoocha Roxb. (heartwood) presented the highest inhibition zone (24 mm), which was similar to chlorhexidine (24-28 mm) and Oxyresveratrol (its pure compound) was found to have the highest effect against S. mutans and presented the largest inhibition zone (20 mm) at the concentration of 30 mg/ml.
Conclusion: Out of forty-three herbal extracts, only Albizia myriophylla Benth. and A. lakoocha Roxb. had superior antimicrobial properties compared to other plants. Oxyresveratrol, which was A. lakoocha Roxb. ’s pure compound did not present better effect than crude extract.
Article Details
How to Cite
1.
Pumpaluk P, Sritularak B, Likhitwitayawuid K, Lapirattanakul J. Antibacterial effect of herbal plants against three cariogenic microorganisms. M Dent J [Internet]. 2016 Dec. 28 [cited 2024 May 4];37(1):71-80. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/180043
Section
Original articles
References
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24. Likhitwitayawuid K, Supudompol B, Sritularak B, Lipipun V, Rapp K, Schinazi RF. Phenolics with anti-HSV and anti-HIV activities from Artocarpus gomezianus, Mallotus pallidus, and Triphasia trifolia. Pharmaceutical Biology 2005; 43: 651-657.
25. Yoshikawa M, Morikawa T, Nakano K, Pongpiriyadacha Y, Murakami T, Matsuda H. Characterization of new sweet triterpene saponins from Albizia myriophylla. Journal of Natural Product 2002; 65: 1638–1642.
26. Joycharat N, Thammavong S, Limsuwan S, Homlaead S, Voravuthikunchai SP, Yingyongnarongkul BE, Dej-Adisai S, Subhadhirasakul S. Antibacterial substances from Albizia myriophylla wood against cariogenic Streptococcus mutans. Archives of Pharmacal Research 2013; 36: 723-730.
27. Haddad M, Laurens V, Lacaille-Dubois MA (2004). Induction of apoptosis in a leukemia cell line by triterpene saponins from Albizia adianthifolia. Bioorganic & Medicinal Chemistry 2004; 12: 4725- 4734.
28. Liu R, Ma S, Yu S, Pei Y, Zhang S, Chen X, Zhang J. Cytotoxic oleanane triterpene saponins from Albizia chinensis. Journal of Natural Product 2009; 72: 632-639.
29. Jung MJ, Chung HY, Kang SS, Choi JH, Bae KS, Choi JS. Antioxidant activity from the stem bark of Albizzia julibrissin. Archive of Pharmacal Research 2003; 26: 458-462.
30. Vaughn K, McClain C, Carrier DJ, Wallace S, King J, Nagarajan S, Clausen E. Effect of Albizia julibrissin water extracts on low density lipoprotein oxidization. Journal of Agricultural and Food Chemistry 2007; 55: 4704-4709.
31. Nagaraja Perumal G, Bhavani Shankar R, Malini S, Soumya V, Janardhanan S, Palanivel V, Senthil Kumar KL. Antiinflammatory and ulcer prtotective activities of chloroform extract of Albizzia chinensis bark and its phytochemical studies. Der Pharmacia Sinica 2010; 1: 95-103.
32. Amornchat C, Kraivaphan P, Dhanabhumi C, Tandhachoon K, Trirattana T, Choonhareongdej S. Effect of Chaem Thai mouthwash on salivary levels of mutans streptococci and total IgA. The Southeast Asian Journal of Tropical Medicine and Public Health 2006; 37: 528-531.
33. Rukayadi Y, Shim JS, Hwang JK. Screening of Thai medicinal plants for anticandidal activity. Mycoses 2008; 51: 308-312.
34. Joycharat N, Boonma C, Muangnua S, Poorisakpaisal P, Suwannawong C, Limsuwan S. Anti-Streptococcus mutans activity of lupinifolin from ethanol extract of Albizia myriophylla Benth. Khon Kaen University Science Journal 2014; 42: 806-819.
35. McDonnell G, Russell AD. Antiseptics and Disinfectants: Activity, Action and Resistance. Clinical Microbiology Reviews 1999; 12: 147-179.
36. Hamilton-Miller JM. Anti-cariogenic properties of tea (Camellia sinensis). Journal of Medical Microbiology 2001; 50: 299-302.
2. Papapanou PN. Periodontal diseases: epidemiology. Annals of Periodontology 1996; 1: 1-36.
3. Lawrence HP, Hunt RJ, Beck JD. Three-year root caries incidence and risk modeling in older adults in North Carolina. Journal Public Health Dentistry 1995; 55: 69-78.
4. Ravald Nand Hamp SE. Prediction of root surface caries in patients treated for advanced periodontal disease. J Clin Periodontol 1981; 8: 400-414.
5. Kitamura M, Kiyak HA, Mulligan K. Predictors of root caries in the elderly. Community Dentistry and Oral Epidemiology 1986; 14: 34-38.
6. Gupta BK, Marya C, Juneja V, Dahiya V. Root Caries: An Aging Problem. The Internet Journal of Dental Science 2007; 5.
7. Shen S, Samaranayake LP, Yip HK, Dyson JE. Bacterial and yeast flora of root surface caries in elderly, ethnic Chinese. Oral Disease 2002; 8: 207-217.
8. Spratt DA, Pratten J. Biofilms and the Oral Cavity. Reviews in Environmental Science and Biotechnology 2003; 2: 109-120.
9. Fure S, Romaniec M, Emilson CG, Krasse B. Proportions of Streptococcus mutans, lactobacilli and Actinomyces spp in root surface plaque. Scandinavian Journal of Dental Research 1987; 95: 119-123.
10. Bowden GH. Microbiology of root surface caries in humans. Journal of Dental Research 1990; 69: 1205-1210.
11. Sumney DL, Jordan HV. Characterization of bacteria isolated from human root surface carious lesion. Journal of Dental Research 1974; 53: 343-351.
12. Schupbach P, Osterwalder V, Guggenheim B. Human root caries: Microbiota of a limited number of root caries lesions. Caries Research 1996; 30: 52 64.
13. Sansone C, Van Houte J, Joshipura K, Kent R, Margolis HC. The association of mutans streptococci and non-mutans streptococci capable of acidigenesis at a low pH with dental caries on enamel and root surfaces. Journal of Dental Research 1993; 72: 508-516.
14. Gaitonde BB, Vaz AX, Patel JR. Chemical and pharmacological study of root bark of Streblus asper Linn. Indian Journal of Medical Research 1964; 8: 191–199.
15. Lewis WH. Plants used as chewing sticks. The Journal of Preventive Dentistry 1980; 6: 71–73.
16. Triratana T, Thaweboon B. The testing of crude extracts of Streblus asper (Koi) against Streptococcus mutans and Streptococcus salivarius. Journal of the Dental Association of Thailand 1987; 37: 19–25.
17. Wongkham S, Laupattarakasaem P, Pienthaweechai K, Areejitranusorn P, Wongkham C, Techanitiswad T. Antimicrobial activity of Streblus asper leaf extract. Phytotherapy Research 2001; 15: 119–121.
18. Nakahara K, Kawabata S, Ono H, Ogura K, Tanaka T, Ooshima T, Hamada S. Inhibitory effect of oolong tea polyphenols on glycosyltransferases of mutans streptococci. Applied and Environmental Microbiology 1993; 59: 968–973.
19. Sritularak B, De-Eknamkul W, Likhitwitayawuid K. Tyrosinase inhibitors from Artocarpus lakoocha. Thai Journal of Pharmaceutical Sciences 1998; 22: 149-155.
20. Suwannalert P, Povichit N, Puchadapirom P Junking M. Anti-Aging Activity and Non-Toxic Dose of Phytooxyresveratrol from Artocarpus lakoocha Roxb. Tropical Journal of Pharmaceutical Research 2012; 11: 69-74.
21. Khare CP. Indian herbal remidies: rational western therapy ayurvedic and other traditional usage botany. Springer-Verlag Berlin Heidelberg 2004.
22. Kim M, Park J, Song K, Kim HG, Koh JS, Boo YC. Screening of plant extracts for human tyrosinase inhibiting effects. International Journal of Cosmetic Science 2012; 34: 202-208.
23. Singhatong, S., Leelarungrayub, D., & Chaiyasut, C., (2010). Antioxidant and toxicity activities of Artocarpus lakoocha Roxb. heartwood extract. Journal of Medicinal Plants Research 2010; 4: 947-953.
24. Likhitwitayawuid K, Supudompol B, Sritularak B, Lipipun V, Rapp K, Schinazi RF. Phenolics with anti-HSV and anti-HIV activities from Artocarpus gomezianus, Mallotus pallidus, and Triphasia trifolia. Pharmaceutical Biology 2005; 43: 651-657.
25. Yoshikawa M, Morikawa T, Nakano K, Pongpiriyadacha Y, Murakami T, Matsuda H. Characterization of new sweet triterpene saponins from Albizia myriophylla. Journal of Natural Product 2002; 65: 1638–1642.
26. Joycharat N, Thammavong S, Limsuwan S, Homlaead S, Voravuthikunchai SP, Yingyongnarongkul BE, Dej-Adisai S, Subhadhirasakul S. Antibacterial substances from Albizia myriophylla wood against cariogenic Streptococcus mutans. Archives of Pharmacal Research 2013; 36: 723-730.
27. Haddad M, Laurens V, Lacaille-Dubois MA (2004). Induction of apoptosis in a leukemia cell line by triterpene saponins from Albizia adianthifolia. Bioorganic & Medicinal Chemistry 2004; 12: 4725- 4734.
28. Liu R, Ma S, Yu S, Pei Y, Zhang S, Chen X, Zhang J. Cytotoxic oleanane triterpene saponins from Albizia chinensis. Journal of Natural Product 2009; 72: 632-639.
29. Jung MJ, Chung HY, Kang SS, Choi JH, Bae KS, Choi JS. Antioxidant activity from the stem bark of Albizzia julibrissin. Archive of Pharmacal Research 2003; 26: 458-462.
30. Vaughn K, McClain C, Carrier DJ, Wallace S, King J, Nagarajan S, Clausen E. Effect of Albizia julibrissin water extracts on low density lipoprotein oxidization. Journal of Agricultural and Food Chemistry 2007; 55: 4704-4709.
31. Nagaraja Perumal G, Bhavani Shankar R, Malini S, Soumya V, Janardhanan S, Palanivel V, Senthil Kumar KL. Antiinflammatory and ulcer prtotective activities of chloroform extract of Albizzia chinensis bark and its phytochemical studies. Der Pharmacia Sinica 2010; 1: 95-103.
32. Amornchat C, Kraivaphan P, Dhanabhumi C, Tandhachoon K, Trirattana T, Choonhareongdej S. Effect of Chaem Thai mouthwash on salivary levels of mutans streptococci and total IgA. The Southeast Asian Journal of Tropical Medicine and Public Health 2006; 37: 528-531.
33. Rukayadi Y, Shim JS, Hwang JK. Screening of Thai medicinal plants for anticandidal activity. Mycoses 2008; 51: 308-312.
34. Joycharat N, Boonma C, Muangnua S, Poorisakpaisal P, Suwannawong C, Limsuwan S. Anti-Streptococcus mutans activity of lupinifolin from ethanol extract of Albizia myriophylla Benth. Khon Kaen University Science Journal 2014; 42: 806-819.
35. McDonnell G, Russell AD. Antiseptics and Disinfectants: Activity, Action and Resistance. Clinical Microbiology Reviews 1999; 12: 147-179.
36. Hamilton-Miller JM. Anti-cariogenic properties of tea (Camellia sinensis). Journal of Medical Microbiology 2001; 50: 299-302.