The effect of silk fibroin hydrogel on proliferation of human stem cells from the apical papilla

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Published: Apr 29, 2019
Keywords:
tissue engineering, scaffold, silk fibroin, stemcell(s), stem cell(s) of apical papilla

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San Sakunphanitphan
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University
Jeeeraphat Jantarat
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University
Hathaitip Sritanaudomchai
Department of Oral Biology, Faculty of Dentistry, Mahidol University
Bovornlak Oonkhanond
Faculty of Engineering, Mahidol University
Kenneth M Hargreaves
Department of Endodontics, University of Texas Health Science Center at San Antonio

Abstract

Objectives :  The aim of this study was to compare the efficiency of various concentrations of silk fibroin hydrogel as a scaffold Proliferation of Human Stem Cells from the Apical Papilla (SCAPs).


 


Materials and Methods : Silk fibroin hydrogel (SF) was prepared at the concentration of 1.5% w/v, 2.0% w/v, and 2.5% w/v.  An individual SF was seeded with SCAPs at 50,000 cells.  Subsequently, MTT assay was used to analyse cell proliferation after 1, 7, 14, and 21 days of culture.  Finally, the morphological features of SCAPs cultured on SF were investigated by live/dead assay.  The Tukcy HSD followed by Dunn test was preformed (P<0.05).


 


Results :  On SF at concentration 1.5%, SCAPs proliferation rate was highest compared to SF at concentration 2.0% and 2.5% significantly (P<0.05).  Moreover, SCAPs on 1.5% SF exhibited more extension of cytoplasmic process and interconnected with neighboring cells than other SF concentration.


 


Conclusion :  The findings from the current study suggest that 1.5% SF had a favorable effect on SCAPs proliferation.  Further studies are required to investigated cell differentiation and the effect of microenvironment (in vivo) on cell and scaffold behavior.


 


 


Keywords: tissue engineering, scaffold, silk fibroin, stemcell(s), stem cell(s) of apical papilla


 


 


 


 


 


 


 

Article Details

How to Cite
1.
Sakunphanitphan S, Jantarat J, Sritanaudomchai H, Oonkhanond B, Hargreaves KM. The effect of silk fibroin hydrogel on proliferation of human stem cells from the apical papilla. M Dent J [Internet]. 2019 Apr. 29 [cited 2024 May 7];39(1):35-40. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/191188
Issue
Vol. 39 No. 1 (2019): January-April
Section
Original articles

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