The effect of silk fibroin hydrogel on proliferation of human stem cells from the apical papilla

Main Article Content

San Sakunphanitphan
Jeeeraphat Jantarat
Hathaitip Sritanaudomchai
Bovornlak Oonkhanond
Kenneth M Hargreaves

Abstract

Objectives :  The aim of this study was to compare the efficiency of various concentrations of silk fibroin hydrogel as a scaffold Proliferation of Human Stem Cells from the Apical Papilla (SCAPs).


 


Materials and Methods : Silk fibroin hydrogel (SF) was prepared at the concentration of 1.5% w/v, 2.0% w/v, and 2.5% w/v.  An individual SF was seeded with SCAPs at 50,000 cells.  Subsequently, MTT assay was used to analyse cell proliferation after 1, 7, 14, and 21 days of culture.  Finally, the morphological features of SCAPs cultured on SF were investigated by live/dead assay.  The Tukcy HSD followed by Dunn test was preformed (P<0.05).


 


Results :  On SF at concentration 1.5%, SCAPs proliferation rate was highest compared to SF at concentration 2.0% and 2.5% significantly (P<0.05).  Moreover, SCAPs on 1.5% SF exhibited more extension of cytoplasmic process and interconnected with neighboring cells than other SF concentration.


 


Conclusion :  The findings from the current study suggest that 1.5% SF had a favorable effect on SCAPs proliferation.  Further studies are required to investigated cell differentiation and the effect of microenvironment (in vivo) on cell and scaffold behavior.


 


 


Keywords: tissue engineering, scaffold, silk fibroin, stemcell(s), stem cell(s) of apical papilla


 


 


 


 


 


 


 

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1.
Sakunphanitphan S, Jantarat J, Sritanaudomchai H, Oonkhanond B, Hargreaves KM. The effect of silk fibroin hydrogel on proliferation of human stem cells from the apical papilla. M Dent J [Internet]. 2019 Apr. 29 [cited 2023 Dec. 11];39(1):35-40. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/191188
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Original articles

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