Effect of different wavelength of LEDs on osteoblast-like cell cultured in 3D collagen type I scaffold

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Published: Jul 19, 2019
Keywords:
Andrographis paniculata gel, chronic periodontitis, doxycycline gel, supportive periodontal therapy, subtraction technique

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Tatsanee Tanglitanont
Private Clinic
Rochaya Chintavalakorn
Mahidol University
Peerapong Santiwong
Department of Orthodontics, Faculty of Dentistry, Mahidol University
Anak Khantachawana
epartment of Mechanical Engineering and Biological Engineering Program, King Mongkut’s University of Technology Thonburi
Kwanchanok Viravaidya Pasuwat
Department of Chemical Engineering and Biological Engineering Program, King Mongkut’s University of Technology Thonburi

Abstract

Objective: To investigates the effect of 4 different wavelengths of light-emitting diodes (LEDs) irradiation on biological response of osteoblast cells (MC3T3-E1) cultured in 3 dimension (3D) collagen type I scaffold.


Materials and methods: The MC3T3-E1 were cultured on 3D type I collagen scaffolds and irradiated daily by LEDs light with wavelengths of 630, 680, 760 and 830 nm for 42 days at   radiant exposure of 3.1 J/cm2 (intensity 2 mW/cm2). The 3D cultured were subjected to biological tests concerning cell proliferation by DNA assay, cell differentiation by alkaline phosphatase (ALP) activity and mineralization by calcium phosphate deposits at day 0th, 7th, 14th, 21st, 28th, 35th and 42th. The 3D cultured at day 42th was investigated by scanning electron microscope (SEM) to determine the effect of LEDs on cell formation.  The mineralization after 42 days in the 3D cultured was evaluated from elemental analysis to determine the ratio of calcium and phosphorus of mineralized granule.


Results: Statistical analysis revealed a significantly higher rate of cell proliferation (p<0.05) in all irradiated cultures in comparison with the controls. The 630 and 680 nm groups yielded a higher number of cells than the 760 and 830 nm (p<0.05).  Cell differentiation, obtained from ALP activity, was increased significantly after 680, 760 and 830 nm irradiation (p<0.05) but decreased after 630 nm irradiation. However, only 680 nm group had significantly greater mineralization than controls (P<0.001) at the end of the experimental period.


Conclusion: The results demonstrate that osteoblastic-liked cells respond to LEDs irradiation differently depending on wavelengths, from 630 to 830 nm, in proliferation and differentiation. To enhance bone mineralization, 680 nm peak irradiated is more effective than those of 630, 760 and 830 nm.

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1.
Tanglitanont T, Chintavalakorn R, Santiwong P, Khantachawana A, Pasuwat KV. Effect of different wavelength of LEDs on osteoblast-like cell cultured in 3D collagen type I scaffold. M Dent J [Internet]. 2019 Jul. 19 [cited 2024 May 4];39(2):143-52. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/199067
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Vol. 39 No. 2 (2019): May-August
Section
Original articles

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