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Objectives: This study aimed to investigate the effect of oil from Ocimum sanctum on cytotoxicity and cell migration of human squamous cell carcinoma.
Materials and methods: The concentrations of Ocimum sanctum oil used in this study were 0.02%, 0.002%, 0.0002% and 0.00002% (v/v). Human squamous cell carcinoma (HSC-4) at a concentration of 1x105 cells/ml was seeded and cultured in 96-well plate. The percentage of cell viability was analyzed by MTT assay and the migration areas were measured using Image-Pro Plus version 7.
Results: It was found that the percentages of human squamous cell carcinoma viability after exposure to 0.02, 0.002, 0.0002 and 0.00002% (v/v) of O. sanctum were 94.44±4.216, 95.13±3.061, 105.55±3.14 and 107.63±3.54, respectively (Table1). Regarding cell migration, all concentrations of O. sanctum could inhibit HSC-4 migration.
Conclusion: O. sanctum oil at concentration of 0.02, 0.002, 0.0002 and 0.00002% (v/v) had no cytotoxic activity on HSC-4 whereas inhibitory effect on cell migration was observed. Our data could be basic knowledge for the benefit of using this oil in oral cancer prevention or treatment. Further studies are still needed to clarify mechanisms of actions of this oil.
Keywords: Cytotoxicity, Migration, Ocimum sanctum, Squamous cell carcinoma
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