Comparison of irrigation techniques on enterococcus faecalis reduction: in vitro study

Article Sidebar

PDF
Published: Nov 12, 2020
DOI: https://doi.org/10.1016/40320207
Keywords:
Enterococcus faecalis, continuous ultrasonic irrigation technique, in vitro, irrigation, molar tooth, passive ultrasonic irrigation technique

Main Article Content

Sirikan Janwattanavej
Department of Endodontics, Maha Chakri Sirindhorn Dental Hospital, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Lalida Ongchavalit
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Ratchapin Laovanitch Srisatjaluk
Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

Abstract

Objectives: To evaluate and compare the antibacterial efficacy of needle irrigation, passive ultrasonic irrigation technique (PUI) and continuous ultrasonic irrigation technique (CUI) in molar teeth.
 
Materials and methods: The mesial roots of 35 mandibular molars were instrumented in a crown-down manner with K3 nickel titanium files to achieve a master apical file size 35/.04. All root canals were contaminated with Enterococcus faecalis . The initial bacteriologic samples were collected from all teeth before irrigation. Specimens were randomly assigned to 3 experimental groups: group 1 NaOCl/needle irrigation, group 2 NaOCl/PUI, and group 3 NaOCl/CUI. Bacteriologic samples were collected immediately after irrigation. The percentages of bacterial reduction from 3 experimental groups were compared (p-value <.05)
 
Results: There was a significant difference in the percentages of bacterial reduction between three experimental groups. The PUI group showed the percentage of bacterial reduction greater than the CUI group, however the percentage of bacterial reduction of needle irrigation group was not significantly different from PUI and CUI groups.
 
Conclusions: All irrigation techniques could reduce a number of bacteria from contaminated root canals.
The PUI group produced greater antimicrobial efficacy than the CUI group.

Article Details

How to Cite
1.
Janwattanavej S, Ongchavalit L, Laovanitch Srisatjaluk R. Comparison of irrigation techniques on enterococcus faecalis reduction: in vitro study. M Dent J [Internet]. 2020 Nov. 12 [cited 2024 Dec. 21];40(3):221-30. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/219118
Issue
Vol. 40 No. 3 (2020): September-December
Section
Original articles

References

Kakehashi S, Stanley HR, Fitzgerald RJ. The Effects of Surgical Exposures of Dental Pulps in Germ-Free and Conventional Laboratory Rats. Oral Surg Oral Med Oral Pathol. 1965; 20: 340-9.

Moller AJ, Fabricius L, Dahlen G, Ohman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 1981; 89: 475-84.

Sjogren U, Hagglund B, Sundqvist G, Wing K. Factors affecting the long-term results of endodontic treatment. J Endod. 1990; 16: 498-504.

Vertucci FJ. Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol. 1984; 58: 589-99.

Bystrom A, Sundqvist G. Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg Oral Med Oral Pathol. 1983; 55: 307-12.

Siqueira JF, Jr., Rocas IN. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97: 85-94.

Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85: 86-93.

Distel JW, Hatton JF, Gillespie MJ. Biofilm formation in medicated root canals. J Endod. 2002; 28: 689-93.

Lee W, Lim S, Son HH, Bae KS. Sonicated extract of Enterococcus faecalis induces irreversible cell cycle arrest in phytohemagglutinin-activated human lymphocytes. J Endod. 2004; 30: 209-12.

Love RM. Enterococcus faecalis--a mechanism for its role in endodontic failure. Int Endod J. 2001; 34: 399-405.

McHugh CP, Zhang P, Michalek S, Eleazer PD. pH required to kill Enterococcus faecalis in vitro. J Endod. 2004; 30: 218-9.

Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod. 2006; 32: 93-8.

Gu LS, Kim JR, Ling J, Choi KK, Pashley DH, Tay FR. Review of contemporary irrigant agitation techniques and devices. J Endod. 2009; 35: 791-804.

Boutsioukis C, Lambrianidis T, Verhaagen B, Versluis M, Kastrinakis E, Wesselink PR, et al. The effect of needle-insertion depth on the irrigant flow in the root canal: evaluation using an unsteady computational fluid dynamics model. J Endod. 2010; 36: 1664-8.

Hulsmann M, Hahn W. Complications during root canal irrigation--literature review and case reports. Int Endod J. 2000; 33: 186-93.

Senia ES, Marshall FJ, Rosen S. The solvent action of sodium hypochlorite on pulp tissue of extracted teeth. Oral Surg Oral Med Oral Pathol. 1971; 31: 96-103.

van der Sluis LW, Versluis M, Wu MK, Wesselink PR. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J. 2007; 40: 415-26.

Moorer WR, Wesselink PR. Factors promoting the tissue dissolving capability of sodium hypochlorite. Int Endod J. 1982; 15: 187-96.

Spoleti P, Siragusa M, Spoleti MJ. Bacteriological evaluation of passive ultrasonic activation. J Endod. 2003; 29: 12-4.

Weber CD, McClanahan SB, Miller GA, Diener-West M, Johnson JD. The effect of passive ultrasonic activation of 2% chlorhexidine or 5.25% sodium hypochlorite irrigant on residual antimicrobial activity in root canals. J Endod. 2003; 29: 562-4.

Al-Jadaa A, Paque F, Attin T, Zehnder M. Acoustic hypochlorite activation in simulated curved canals. J Endod. 2009; 35: 1408-11.

de Gregorio C, Estevez R, Cisneros R, Heilborn C, Cohenca N. Effect of EDTA, sonic, and ultrasonic activation on the penetration of sodium hypochlorite into simulated lateral canals: an in vitro study. J Endod. 2009; 35: 891-5.

de Gregorio C, Estevez R, Cisneros R, Paranjpe A, Cohenca N. Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study. J Endod. 2010; 36: 1216-21.

van der Sluis LW, Gambarini G, Wu MK, Wesselink PR. The influence of volume, type of irrigant and flushing method on removing artificially placed dentine debris from the apical root canal during passive ultrasonic irrigation. Int Endod J. 2006; 39: 472-6.

van der Sluis LW, Shemesh H, Wu MK, Wesselink PR. An evaluation of the influence of passive ultrasonic irrigation on the seal of root canal fillings. Int Endod J. 2007; 40: 356-61.

van der Sluis LW, Wu MK, Wesselink PR. The evaluation of removal of calcium hydroxide paste from an artificial standardized groove in the apical root canal using different irrigation methodologies. Int Endod J. 2007; 40: 52-7.

Burleson A, Nusstein J, Reader A, Beck M. The in vivo evaluation of hand/rotary/ultrasound instrumentation in necrotic, human mandibular molars. J Endod. 2007; 33: 782-7.

Carver K, Nusstein J, Reader A, Beck M. In vivo antibacterial efficacy of ultrasound after hand and rotary instrumentation in human mandibular molars. J Endod. 2007; 33: 1038-43.

Gutarts R, Nusstein J, Reader A, Beck M. In vivo debridement efficacy of ultrasonic irrigation following hand-rotary instrumentation in human mandibular molars. J Endod. 2005; 31: 166-70.

Siqueira JF, Jr., Rocas IN. Exploiting molecular methods to explore endodontic infections: Part 2--Redefining the endodontic microbiota. J Endod. 2005; 31: 488-98.

Basmaci F, Öztan MD, Kiyan M. Ex vivo evaluation of various instrumentation techniques and irrigants in reducing E. faecalis within root canals. Int Endod J. 2013; 46: 823-30.

Castelo-Baz P, Martin-Biedma B, Cantatore G, Ruiz-Pinon M, Bahillo J, Rivas-Mundina B, et al. In vitro comparison of passive and continuous ultrasonic irrigation in simulated lateral canals of extracted teeth. J Endod. 2012; 38: 688-91.

Jiang LM, Lak B, Eijsvogels LM, Wesselink P, van der Sluis LW. Comparison of the cleaning efficacy of different final irrigation techniques. J Endod. 2012; 38: 838-41.

Curtis TO, Sedgley CM. Comparison of a continuous ultrasonic irrigation device and conventional needle irrigation in the removal of root canal debris. J Endod. 2012; 38: 1261-4.

Adcock JM, Sidow SJ, Looney SW, Liu Y, McNally K, Lindsey K, et al. Histologic evaluation of canal and isthmus debridement efficacies of two different irrigant delivery techniques in a closed system. J Endod. 2011; 37: 544-8.

Howard RK, Kirkpatrick TC, Rutledge RE, Yaccino JM. Comparison of debris removal with three different irrigation techniques. J Endod. 2011; 37: 1301-5.

Chow TW. Mechanical effectiveness of root canal irrigation. J Endod. 1983; 9: 475-9.

Sedgley CM, Nagel AC, Hall D, Applegate B. Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real-time imaging in vitro. Int Endod J. 2005; 38: 97-104.