Correlation between cell free DNA in gingival crevicular fluid and clinical periodontal parameters by using two collection techniques

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Sukhumchawee Suwannagindra
Boonyanit Thaweboon
Varunee Kerdvongbundit


Objectives: The purpose of this study was to evaluate the correlation between cell free DNA (cfDNA) in gingival crevicular fluid (GCF) and clinical periodontal parameters and to compare the efficiency of the two GCF collection techniques.

Materials and Methods: Clinical periodontal parameters including pocket depth, clinical attachment level and bleeding on probing were recorded in forty teeth of twenty patients with moderate to severe periodontal disease. GCF samples were collected from each tooth by two collection techniques. First, it was randomly collected by either modified washing technique or absorbent paper strips. Following one week, the samples were recollected from the same tooth with another technique. The obtained samples were centrifuged to obtain cfDNA in the supernatant, from which DNA extraction was performed by using InstaGene Matrix. The concentration of cfDNA was measured by nanodrop spectrophotometer and processed to polymerase chain reaction with specific primers for 110, 536, and 2,000 bp of human β-globin gene.

Results: There was no significant difference in cfDNA concentration between the two collection techniques.
Furthermore, these cfDNA concentrations were not significantly correlated with any clinical periodontal
parameters. Interestingly, 2,000 bp PCR products showed positive correlation among all clinical periodontal
parameters (p<0.05). The presence of this PCR product was significant to the level of severity of the clinical

Conclusion: The cell free DNA collected by absorbent paper strips was as good as by modified washing technique. Therefore, the absorbent paper strips could be used for GCF collection instead of the modified washing technique. The presence of 2,000 bp PCR product, in GCF may be related to the severity of periodontal diseases.


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Suwannagindra S, Thaweboon B, Kerdvongbundit V. Correlation between cell free DNA in gingival crevicular fluid and clinical periodontal parameters by using two collection techniques. M Dent J [Internet]. 2020 Aug. 28 [cited 2023 Dec. 7];40(2):165-74. Available from:
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