Biocompatibility of a chitosan-derived hemostatic agent with human alveolar osteoblasts

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Kaltimas Phaewphala
Wasana Kosorn
Wanida Janvikul
Kitwat Kitrueangphatchara
Marnisa Sricholpech


Objective: To evaluate the biocompatibility of a Calcium Alginate/N,O-carboxymethylchitosan (CA/NOCC)
hemostatic sponge with primary human alveolar osteoblasts (hAOBs).

Materials and Methods: The primary hAOBs, isolated from alveolar bone chips, were cultured with or without the CA/NOCC sponge. Cell morphology was assessed by scanning electron microscopy, while cell proliferation was determined by MTT assay. The effects of the CA/NOCC sponge extract on osteoblastic phenotypes were evaluated with alkaline phosphatase activity and matrix mineralization assays.

Results: Primary hAOBs, cultured with the CA/NOCC sponge, showed comparable cell morphology to the control cells with minimal cell attachments to the material surface. In addition, the proliferative ability of the cells was relatively unaltered. Moreover, the hAOBs cultured in the osteogenic CA/NOCC extract exhibited a comparable ALP activity level, while the extent of mineral deposition was slightly lower than the control.

Conclusion: Our results indicated that the CA/NOCC hemostatic sponge is biocompatible to hAOBs and
could potentially be a promising material for bleeding control in the jaw bone.


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Phaewphala K, Kosorn W, Janvikul W, Kitrueangphatchara K, Sricholpech M. Biocompatibility of a chitosan-derived hemostatic agent with human alveolar osteoblasts. M Dent J [Internet]. 2020 Nov. 16 [cited 2023 Dec. 11];40(3):277-88. Available from:
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