Biocompatibility of a chitosan-derived hemostatic agent with human alveolar osteoblasts
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Abstract
Objective: To evaluate the biocompatibility of a Calcium Alginate/N,O-carboxymethylchitosan (CA/NOCC)
hemostatic sponge with primary human alveolar osteoblasts (hAOBs).
Materials and Methods: The primary hAOBs, isolated from alveolar bone chips, were cultured with or without the CA/NOCC sponge. Cell morphology was assessed by scanning electron microscopy, while cell proliferation was determined by MTT assay. The effects of the CA/NOCC sponge extract on osteoblastic phenotypes were evaluated with alkaline phosphatase activity and matrix mineralization assays.
Results: Primary hAOBs, cultured with the CA/NOCC sponge, showed comparable cell morphology to the control cells with minimal cell attachments to the material surface. In addition, the proliferative ability of the cells was relatively unaltered. Moreover, the hAOBs cultured in the osteogenic CA/NOCC extract exhibited a comparable ALP activity level, while the extent of mineral deposition was slightly lower than the control.
Conclusion: Our results indicated that the CA/NOCC hemostatic sponge is biocompatible to hAOBs and
could potentially be a promising material for bleeding control in the jaw bone.
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References
Kamoh A and Swantek J. Hemostasis in oral surgery. Dental Clinics. 2012; 56: 17-23.
Kumar S. Local hemostatic agents in the management of bleeding in oral surgery. Asian J Pharm Clin Res. 2016; 9: 35-41.
Khan MA and Mujahid M. A review on recent advances in chitosan based composite for hemostatic dressings. Int J Biol Macromol. 2019; 124: 138-147.
Cicciù M, Fiorillo L and Cervino G. Chitosan Use in Dentistry: A Systematic Review of Recent Clinical Studies. Mar Drugs. 2019; 17: 417.
Koksal O, Ozdemir F, Cam Etoz B, Buyukcoskun NI and Sigirli D. Hemostatic effect of a chitosan linear polymer (Celox®) in a severe femoral artery bleeding rat model under hypothermia or warfarin therapy. Ulus Travma Acil Cerrahi Derg. 2011; 17: 199-204.
Millner RW, Lockhart AS, Marr R and Jones K. Omni-Stat®(Chitosan) arrests bleeding in heparinised subjects in vivo: an experimental study in a model of major peripheral vascular injury. Eur J Cardio-Thorac. 2011; 39: 952-954.
Shariatinia Z. Carboxymethyl chitosan: Properties and biomedical applications. Int J Biol Macromol. 2018; 120: 1406-1419.
Janvikul W and Thavornyutikarn B. New Route to the Preparation of Carboxymethylchitosan Hydrogels. J Appl Polym Sci. 2003; 90: 4016-4020.
Janvikul W, Uppanan P, Thavornyutikarn B, Krewraing J and Prateepasen R. In vitro comparative hemostatic studies of chitin, chitosan, and their derivatives. J Appl Polym Sci. 2006; 102: 445-451.
Thavornyutikarn B, Kosorn W, Surattanawanich P, Wongparami U and Janvikul W. Chitosan derivative-based hemostat: Irritation, hypersensitivity and absorbability study. J Met Mater Miner. 2013; 23: 49-52.
Janvikul W, Uppanan P, Kosorn W, Phusuksombati D and Prateepasen R. Evaluation of Efficacy of Chitosan Derivative Based Hemostat: In Vitro and In Vivo Studies. Proceedings of the 2nd International Symposium on Biomedical Engineering (ISBME 2006). 2006, November 8-10: 281-283.
Janvikul W, Uppanan P, Thavornyutikarn B and Prateepasen R. Calcium Alginate, N,O carboxymethylchitosan and Their Blends: In Vitro Biocompatibility and Biodegradability. The World Congress on Bioengineering 2007 (WACBE). 2007.
Janvikul W, Thavornyutikarn B, Kosorn W and Surattanawanich P. Clinical study of chitosan-derivative-based hemostat in the treatment of split-thickness donor sites. Maejo Int J Sci and Technol. 2013; 7: 385-95.
Stacey G. Primary cell cultures and immortal cell lines. e LS. 2001. https://doi.org/10.1038/npg.els.0003960.
Gregory CA, Gunn WG, Peister A and Prockop DJ. An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Anal Biochem. 2004; 329: 77-84.
Lim SM, Yap A, Loo C, Ng J, Goh CY, Hong C, et al. Comparison of cytotoxicity test models for evaluating resin-based composites. Hum Exp Toxicol. 2017; 36: 339-348.
Eldeniz AU, Mustafa K, Orstavik D and Dahl JE. Cytotoxicity of new resin-, calcium hydroxide- and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines. Int Endod J. 2007; 40: 329-37.
Elson C and Lee TD. Adhesive N, O-carboxymethylchitosan coatings which inhibit attachment of substrate-dependent cells and proteins. 2007. US Patent 7,238,678, Jul 3.
Zhou J, Liwski RS, Elson C and Lee TD. Reduction in postsurgical adhesion formation after cardiac surgery in a rabbit model using N,O-carboxymethyl chitosan to block cell adherence. J Thorac Cardiovasc Surg. 2008; 135: 777-83.
Alsberg E, Anderson KW, Albeiruti A, Franceschi RT and Mooney DJ. Cell-interactive alginate hydrogels for bone tissue engineering. J Dent Res. 2001; 80: 2025-9.
Rubert M, Monjo M, Lyngstadaas SP and Ramis JM. Effect of alginate hydrogel containing polyproline-rich peptides on osteoblast differentiation. Biomed Mater. 2012; 7: 055003.
Wang L, Shelton RM, Cooper PR, Lawson M, Triffitt JT and Barralet JE. Evaluation of sodium alginate for bone marrow cell tissue engineering. Biomaterials. 2003; 24: 3475-81.
Li Z, Ramay HR, Hauch KD, Xiao D and Zhang M. Chitosan–alginate hybrid scaffolds for bone tissue engineering. Biomaterials. 2005; 26: 3919-3928.
Fernandes MH, Costa MA and Carvalho GS. Mineralization in serially passaged human alveolar bone cells. J Mater Sci Mater Med. 1997; 8: 61-5.
Marolt D, Rode M, Kregar-Velikonja N, Jeras M and Knezevic M. Primary human alveolar bone cells isolated from tissue samples acquired at periodontal surgeries exhibit sustained proliferation and retain osteogenic phenotype during in vitro expansion. PLoS One. 2014; 9: e92969-e92969.
Mochida Y, Parisuthiman D, Pornprasertsuk-Damrongsri S, Atsawasuwan P, Sricholpech M, Boskey AL, et al. Decorin modulates collagen matrix assembly and mineralization. Matrix Biol. 2009; 28: 44-52.
Parisuthiman D, Mochida Y, Duarte WR and Yamauchi M. Biglycan modulates osteoblast differentiation and matrix mineralization. J Bone Miner Res. 2005; 20: 1878-86.
Lu HT, Lu TW, Chen CH, Lu KY and Mi FL. Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering. Int J Biol Macromol. 2018; 120: 2335-2345.
Muller WEG, Tolba E, Schroder HC, Neufurth M, Wang S, Link T, et al. A new printable and durable N,O-carboxymethyl chitosan-Ca(2+)-polyphosphate complex with morphogenetic activity. J Mater Chem B. 2015; 3: 1722-1730.
Li X, Kong X, Zhang Z, Nan K, Li L, Wang X, et al. Cytotoxicity and biocompatibility evaluation of N,O-carboxymethyl chitosan/oxidized alginate hydrogel for drug delivery application. Int J Biol Macromol. 2012; 50: 1299-305.
Taskin AK, Yasar M, Ozaydin I, Kaya B, Bat O, Ankarali S, et al. The hemostatic effect of calcium alginate in experimental splenic injury model. Ulus Travma Acil Cerrahi Derg. 2013; 19: 195-199.
Sun J and Tan H. Alginate-Based Biomaterials for Regenerative Medicine Applications. Materials (Basel). 2013; 6: 1285-1309.
Rosdy M and Clauss LC. Cytotoxicity testing of wound dressings using normal human keratinocytes in culture. J Biomed Mater Res. 1990; 24: 363-77.
Suzuki Y, Nishimura Y, Tanihara M, Suzuki K, Nakamura T, Shimizu Y, et al. Evaluation of a novel alginate gel dressing: cytotoxicity to fibroblasts in vitro and foreign-body reaction in pig skin in vivo. J Biomed Mater Res. 1998; 39: 317-22.
Paddle-Ledinek JE, Nasa Z and Cleland HJ. Effect of different wound dressings on cell viability and proliferation. Plast Reconstr Surg. 2006; 117: 110S-118S; discussion 119S-120S.
Chen C-Y, Ke C-J, Yen K-C, Hsieh H-C, Sun J-S and Lin F-H. 3D porous calcium-alginate scaffolds cell culture system improved human osteoblast cell clusters for cell therapy. Theranostics. 2015; 5: 643.
Schloßmacher U, Schröder HC, Wang X, Feng Q, Diehl-Seifert B, Neumann S, et al. Alginate/silica composite hydrogel as a potential morphogenetically active scaffold for three-dimensional tissue engineering. RSC advances. 2013; 3: 11185-11194.
Blair HC, Larrouture QC, Li Y, Lin H, Beer-Stoltz D, Liu L, et al. Osteoblast Differentiation and Bone Matrix Formation In Vivo and In Vitro. Tissue Eng Part B Rev. 2017; 23: 268-280.
Ma S, Yang Y, Carnes DL, Kim K, Park S, Oh SH, et al. Effects of dissolved calcium and phosphorous on osteoblast responses. J Oral Implantol. 2005; 31: 61-7.
Maeno S, Niki Y, Matsumoto H, Morioka H, Yatabe T, Funayama A, et al. The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials. 2005; 26: 4847-55.