The efficacy of handwashing gel containing banana peel extract in reducing bacterial load on dentists’ hands

Main Article Content

Duangkaew Kerdsiri
Nada Kengpanich
Benyatip Duangsombat
Pornpimon Kamchai
Wilairat Worapamorn

Abstract

Objective: This study aimed to investigate the efficacy of hand-washing gel containing banana peel extract in reducing bacterial load on dentists’ hands.


Materials and Methods: Twenty-four volunteers participated in this crossover study using 4% chlorhexidine gluconate, gel with banana peel extract, and gel without banana peel extract. At each round of the trial, the volunteers washed their hands with 4% chlorhexidine gluconate, put on gloves, and provided dental treatments, i.e. operative treatment, scaling and root planing, endodontic work, or prosthodontic procedures. After 30 minutes, the volunteers took off gloves and firmly put thumbs for 5 seconds on the first nutrient agar plates (baseline). The volunteers in each group then washed their hands with the assigned hand-washing products and firmly put thumbs for 5 seconds on the second agar plates (immediately washing the hands). The volunteers put on gloves and continued to do their work. After one hour, the volunteers took off gloves and firmly put thumbs for 5 seconds on the third agar plates (one hour after washing the hands). After 24-hours incubation at 35°C, the bacterial colonies on agar plates were observed and counted. Bacterial loads on hands were compared using Wilcoxon Signed Ranks test and Mann-Whitney U test.


Results: The results showed that the number of bacterial colonies was significantly decreased from baseline to immediately washing the hands with 4% chlorhexidine gluconate and gel with banana peel extract but not for the gel without banana peel extract. However, the number of bacterial colonies was significantly increased from immediately to one hour washing the hands for all assigned handwashing products.


Conclusion: The data from this study suggests that handwashing gel containing banana peel extract has the promising results in reducing bacterial load on dentist’s hands. This finding supports the utilization of banana peels, a vast agricultural residue in Thailand, as anti-bacterial agent.

Article Details

How to Cite
1.
Kerdsiri D, Kengpanich N, Duangsombat B, Kamchai P, Worapamorn W. The efficacy of handwashing gel containing banana peel extract in reducing bacterial load on dentists’ hands. M Dent J [Internet]. 2021 Aug. 18 [cited 2024 Nov. 21];41(2):113-21. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/245796
Section
Original articles

References

Anargh V, Singh H, Kulkarni A, Kotwal A, Mahen A. Hand hygiene practices among health care workers (HCWs) in a tertiary care facility in Pune. Med J armed forces India 2013; 69: 54-56.

Pittet D, Dharan S, Tauveneau S, Sauvan V, Perneger TV. Bacterial contamination of the hands of hospital staff during routine patient care. Arch Intern Med 1999; 159: 821-26.

Anitha M, Hema priya J, Swathy SR, Pavithra GB. Prevalence of hand hygiene among various categories of healthcare workers in hospital setting. Inter J App Res 2015; 1: 96-99.

Watutantrige RDA, Premalatha P, Lum WS, Evelyn CX. A study on hand contamination and hand washing practices among medical students. Inter scholarly Res Net 2012; 1-5.

World Health Organization. WHO Guidelines on Hand Hygiene in Health Care: a Summary. WHO Press, 20 Avenue Appia, 1211 Geneva 27, Switzerland.

Baraldi MM, Rizzo JG, Padoveze MC. Risks and benefits of using chlorhexidine gluconate in handwashing. A systematic literature review Am J Infect Control 2019; 47: 704-14.

Simmonds NW, Shepherd K. Taxonomy and origins of cultivated bananas. Bot J Lin Soc 2008; 55: 302–12.

Aboul-Enein AM, Salama ZA, Gaafar AA, Aly HF, A bou-Elella F, Ahmed HA. Identification of phenolic compounds from banana peel (Musa paradaisica L.) as antioxidant and antimicrobial agents. J Chem Pharm Res 2016; 8: 46-55.

Ehiowemwenguan G, Emoghene AO, Inetianbor JE. Antibacterial and phytochemical analysis of banana fruit peel. IOSR J Pharm 2014; 4: 18-25.

Surojanamethaku V. Extraction of tannin from banana peel. Bangkok:Kasetsat university 2004 [accessed 2020 April 8]. Available from: http://agris.fao.org/agris-search/search.do%3FrecordID%3DTH2001000960

Mokbel MS, Hashinaga F. Antibacterial and antioxidant activities of banana (Musa, AAA cv. Cavendish) fruits peel. Am J Biochem Biotechnol 2005; 3: 125-31.

Taper AR, Jain RK. Study of advanced maturity stages of banana. Int J Adv Eng Res Stu 2012; 1: 272-74.

Wani NS, Bhalerao AK, Ranaware VP, Zanje R. Formulation and evaluation of herbal sanitizer. Int J Pharm Tech Res 2013; 5: 40-43.

Habib F, Rind R, Durani N, Bhutto AL, Buriro RS, Aijaz N, et al. Morphological and cultural characterization of Staphylococcus aureus isolated from different animal species J App Environ Biol Sci 2015; 5: 15-26.

Hernandes SE, Mello AC, Sant'Ana JJ, Soares VS, Cassiolato V, Garcia LB, et al. The effectiveness of alcohol gel and other hand-cleansing agents against important nosocomial pathogens. Braz J Microbiol 2004; 35: 33-39.

Hajipour L, Longstaff L, Cleeve V, Brewster N, Bint D, Henman P. Hand washing rituals in trauma theatre: clean or dirty? Ann R Coll Surg Engl 2006; 88: 13–15. doi 10.1308/003588406X83032

Stauffer F, Griess M, Pleininger G, Zhumadilova A, Assadian O. Acceptability and tolerability of liquid versus gel and standard versus virucidal alcohol-based hand rub formulations among dental students. Am J Infect cont 2013; 41: 1007-11.

Traore O, Hugonnet S, Lübbe J, Griffiths W, Pittet D. Liquid versus gel handrub formulation: a prospective intervention. Crit care 2007; 11: 1-8.

Wagner S, Breiteneder H. The latex-fruit syndrome. Biochem Soc Trans 2002; 30: 935-40.

Delbourg MF, Guilloux L, Moneret-Vautrin DA, Ville G. Hypersensitivity to Banana in Latex-Allergic Patients. Identification of Two Major Banana Allergens of 33 and 37 kD. Ann allergy asthma immunol 1996; 76: 321-26.