n vitro antibacterial activity of oligomer-based and calcium silicate-based root canal sealers
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Abstract
Objective: To evaluate antibacterial effects of root canal sealers, oligomer-based (OB) and calcium silicate (BC)
in comparison to epoxy-resin (AH Plus) and zinc oxide-eugenol (ZOE), against Enterococcus faecalis.
Materials and Methods: Antibacterial effects of the root canal sealers were evaluated by the modified direct
contact method using 96-well plates. Each sealer was filled on the bottom of wells at 1-mm thickness, 20 wells
of each sealer. The wells of each group was further divided into five subgroups depending on testing periods
after sealer setting, i.e.- 20 min, 24 h, 3, 7, and 14 days. Next, 200-µl aliquot of E. faecalis (5 x 105 CFU) was
placed in the well containing the set sealer and kept at 37°C for 24 h. The wells containing the bacterial
suspension (without any sealer) and sterile culture media were used as positive and negative controls. Survival
of bacteria were determined by 10-fold serial dilution in Brain-Heart Infusion (BHI) broth and cultured on BHI
agar. In addition, elution test was carried out by incubating the bacterial suspension to culture media that
exposed to the set sealers for 20 min, 24 h, and 3 days. Statistical analysis was conducted using Kruskal-Wallis
non-parametric test (α=.05).
Results: From the direct contact test, at 20 min and 24h, BC sealer and AH Plus showed strong bactericidal
effects while OB sealer did not display any antibacterial effect. At 3 days, antimicrobial effect of BC sealer was
significantly reduced while AH Plus did not show the antibacterial effect. At 7 and 14 days, all sealers did not
possess any antibacterial activity, except ZOE sealer that had exhibited the potent bactericidal effect until 14
days. For the elution test, eluted substances from the test sealers at all setting periods did not cause any
significant reduction of the bacteria.
Conclusion: The root canal sealers showed different antimicrobial activity against E. faecalis after setting. OB
sealer showed no antibacterial effects at all periods. ZOE sealer was the most effective sealer with antibacterial
activity until 14 days. Antimicrobial effects of AH Plus and BC sealers gradually decreased within 24 h and 3
days after setting, respectively.
Article Details
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