The proliferative effect of conditioned media from human periodontal ligament stem cells and human exfoliated deciduous teeth stem cells on human gingival fibroblasts
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Abstract
Objective: This study aimed to investigate the potential of conditioned medium (CM) from human periodontal ligament stem cells (PDLSCs) and CM from stem cells from human exfoliated deciduous teeth (SHEDs) to promote the proliferation of human gingival fibroblasts (HGFs).
Materials and Methods: The PDLSC-CM, SHED-CM and HGF-CM were obtained from cell culture supernatants on day 2 and day 4. The amount of protein in each sample was determined using the Bradford assay. For the cell viability assay, HGFs were seeded in 96-well plates at a density of 1.2x104 cells/well and treated with various concentrations of PDLSC-CM, SHED-CM and HGF-CM (8.75, 17.5, 35, 70, 140, or 280 µg/ml) in DMEM without serum for 24 hours. For the proliferation assay, HGFs were seeded in 96-well plates at a density of 2x103 cells/ well and treated with 8.75, 17.5, or 35 µg/ml PDLSC-CM, SHED-CM and HGF-CM in DMEM with serum. The proliferation of HGFs on days 1, 2, 4, 6, and 8 after treatment was measured.
Results: The cell viability of HGFs treated with PDLSC-CM at 8.75 µg/ml showed significantly greater cell viability
than those treated with SHED-CM and HGF-CM at the same concentration. The proliferation assay showed that HGFs treated with a lower concentration of PDLSC-CM (8.75 µg/ml) had higher proliferation than cells treated with SHED-CM and HGF-CM at the same concentrations at day 4. Interestingly, HGFs treated with PDLSC-CM at 17.5 and 35 µg/ml exhibited higher proliferation than those treated with SHED-CM and HGF-CM for a longer period of time (4-6 days).
Conclusion: Our results suggest that PDLSC-CM enhances HGF proliferation more effectively than SHED-CM
and that PDLSC-CM may be used as the promising agent for further studies on the biological properties of HGFs, which would be an advantage for oral and periodontal tissue healing and regeneration.
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References
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